Paper · 2024 · Journal of Experimental and Molecular Biology

Applications of combinations and concentrations of different phytohormones for the induction of callus, shoot, and root of cannabis sativaunder tissue culture technique

Muhammad Waqar Mazhar; Sadaf Javed

5
claims
7
verbatim quotes
1
citations
2
research areas
✓
Open access · cc-by

Abstract

Cannabis sativa is a significant crop of the family (cannabaceae).Interest has been resurgent in the usage of the cannabis plant for medical purposes, industrial fibre, seed oil, food, and recreation.Auxin and cytokinins act antagonistically with each other in which cytokinins promote shoot branching and inhibit root branching, while auxin inhibits shoot branching and promotes root branching. Young cannabis leaves and petioles were used and inoculated in the MS medium with various combinations. Murashige and Skoog (MS) medium for explant were developed in additions of 25µl-450µl 2, 4 D, 25µl-450µl Kinetin, and 50µl-450µl BAP for callus induction. Shoot induction was developed on 2-3ml/l BAP-supplemented MS media from the callus. Root induction was developed from the shoot on MS5ml/2 litters (indole-3-acetic acid) IAA + 8ml/2 litters (indole-3-butyric acid)IBA-supplemented media.Seeds of cannabis were inoculated in the MS medium. After one week, seed germination was observed in the seeds of the DG Khan variety of Cannabis. MS media found better and more abundant Callus induction with 450µl 2, 4D +450µl Kinetin+450µlBAP from cannabis sativa leaves. 3ml/l BAP observed the high growth of the shoot. Better growth of roots was observed by 5ml/2 litters (indole-3-acetic acid) IAA + 8ml/2 litters (indole-3-butyric acid) IBA. In the present study, seed, callus, shoot, root, and meristem growth were observed using the combination and concentration of different growth regulators. By using different phytohormones, the growth rate and productivity of Cannabiswere significantly increased, which can be valuable for medical purposes.

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Claims 5

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

DG Khan cannabis seeds germinated after one week on MS medium.

Taxon
CannabisCannabise0
Material
DG Khan varietye0
Outcome
seed germinatione0
Qualifier
After one weeke0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis
Cannabis · Taxon · picture of the genus (one of its species)United States Fish and Wildlife Service · Public domain · Wikimedia Commons ↗

Verbatim

e0

“After one week, seed germination was observed in the seeds of the DG Khan variety of Cannabis”

✓ located verbatim in the source · abstract, characters 943–1036 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The 450 µl 2,4D, Kinetin and BAP combination produced better, more abundant callus from cannabis leaves.

Taxon
cannabis sativaCannabis sativae0
Organ / stage
leavese0
Treatment
450µl 2, 4D +450µl Kinetin+450µlBAPe0
Outcome
Callus inductione0
Direction
better and more abundante0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“MS media found better and more abundant Callus induction with 450µl 2, 4D +450µl Kinetin+450µlBAP from cannabis sativa leaves”

✓ located verbatim in the source · abstract, characters 1038–1163 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BAP at 3 ml/l produced high shoot growth.

Taxon
cannabis sativaCannabis sativat0
Treatment
3ml/l BAPe0
Outcome
growth of the shoote0
Direction
highe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“3ml/l BAP observed the high growth of the shoot”

✓ located verbatim in the source · abstract, characters 1165–1212 · show in text ↑
t0

“cannabis sativa”

✓ located verbatim in the source · title, characters 123–138 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

IAA and IBA supplementation yielded better root growth.

Taxon
cannabis sativaCannabis sativat0
Treatment
5ml/2 litters (indole-3-acetic acid) IAA + 8ml/2 litters (indole-3-butyric acid) IBAe0
Outcome
growth of rootse0
Direction
Bettere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa
Cannabis sativa · TaxonThayne Tuason · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Better growth of roots was observed by 5ml/2 litters (indole-3-acetic acid) IAA + 8ml/2 litters (indole-3-butyric acid) IBA”

✓ located verbatim in the source · abstract, characters 1214–1337 · show in text ↑
t0

“cannabis sativa”

✓ located verbatim in the source · title, characters 123–138 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Different phytohormones significantly increased cannabis growth rate and productivity.

Taxon
CannabisCannabise0
Treatment
different phytohormonese0
Outcome
growth rate and productivitye0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis
Cannabis · Taxon · picture of the genus (one of its species)United States Fish and Wildlife Service · Public domain · Wikimedia Commons ↗

Verbatim

e0

“By using different phytohormones, the growth rate and productivity of Cannabiswere significantly increased”

✓ located verbatim in the source · abstract, characters 1494–1600 · show in text ↑

N-benzyladenine · growth · Cannabis sativaPositive and negative results

7 papers: 6 positive, 1 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. no effect
  3. positive

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indole-3-butyric acid · growth · Cannabis sativaPositive and negative results

11 papers: 10 positive, 1 negative or no effect

9 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

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indole-3-acetic acid · growth · Cannabis sativaResults agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.