Abstract
A protocol for high-frequency direct organogenesis from root explants of Kachai lemon (Citrus jambhiri Lush.) was developed. Full-length roots (~3 cm) were isolated from the in vitro grown seedlings and cultured on Murashige and Skoog basal medium supplemented with Nitsch vitamin (MSN) with different concentrations of cytokinin [6-benzylaminopurine, (BAP)] and gibberellic acid (GA3). The frequency of multiple shoot proliferation was very high, with an average of 34.3 shoots per root explant when inoculated on the MSN medium supplemented with BAP (1.0 mg L-1) and GA3 (1.0 mg L-1). Optimal rooting was induced in the plantlets under half strength MSN medium supplemented with indole-3-acetic acid (IAA, 0.5-1.0 mg L-1). IAA induced better root structure than 1-naphthaleneacetic acid (NAA), which was evident from the scanning electron microscopy (SEM). The expressions of growth regulating factor genes (GRF1 and GRF5) and GA3 signaling genes (GA2OX1 and KO1) were elevated in the regenerants obtained from MSN+BAP (1.0 mg L-1)+GA3 (1.0 mg L-1). The expressions of auxin regulating genes were high in roots obtained in ½ MSN+IAA 1.0 mg L-1. Furthermore, indexing of the regenerants confirmed that there was no amplicons detected for Huanglongbing bacterium and Citrus tristeza virus. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers detected no polymorphic bands amongst the regenerated plants. This is the first report that describes direct organogenesis from the root explant of Citrus jambhiri Lush. The high-frequency direct regeneration protocol in the present study provides an enormous significance in Citrus organogenesis, its commercial cultivation and genetic conservation.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
MSN medium with BAP and GA3 produced an average of 34.3 shoots per root explant.
- Taxon
- Citrus jambhiri Lush.
.jpg?width=60)
Citrus jambhirit0 - Treatment
- MSN medium supplemented with BAP (1.0 mg L-1) and GA3 (1.0 mg L-1)e0
- Outcome
- multiple shoot proliferatione0
- Direction
- very highe0
- Value
- 34.3 shoots per root explante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The frequency of multiple shoot proliferation was very high, with an average of 34.3 shoots per root explant when inoculated on the MSN medium supplemented with BAP (1.0 mg L-1) and GA3 (1.0 mg L-1).”
t0“Citrus jambhiri Lush.”
Claim · model-written summary
Half-strength MSN with IAA induced optimal rooting.
- Taxon
- Citrus jambhiri Lush.Citrus jambhirit0
- Treatment
- half strength MSN medium supplemented with indole-3-acetic acid (IAA, 0.5-1.0 mg L-1)e0
- Outcome
- rootinge0
- Direction
- Optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal rooting was induced in the plantlets under half strength MSN medium supplemented with indole-3-acetic acid (IAA, 0.5-1.0 mg L-1).”
t0“Citrus jambhiri Lush.”
Claim · model-written summary
IAA induced better root structure than NAA.
- Taxon
- Citrus jambhiri Lush.Citrus jambhirit0
- Treatment
- IAAe0
- Outcome
- root structuree0
- Direction
- bettere0
- Comparator
- 1-naphthaleneacetic acid (NAA)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA induced better root structure than 1-naphthaleneacetic acid (NAA), which was evident from the scanning electron microscopy (SEM).”
t0“Citrus jambhiri Lush.”
Claim · model-written summary
Regenerants from MSN+BAP+GA3 had elevated GRF1 and GRF5 expression.
- Taxon
- Citrus jambhiri Lush.Citrus jambhirit0
- Treatment
- MSN+BAP (1.0 mg L-1)+GA3 (1.0 mg L-1)e0
- Gene
- GRF1 and GRF5e0
- Outcome
- expressions of growth regulating factor genese0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expressions of growth regulating factor genes (GRF1 and GRF5) and GA3 signaling genes (GA2OX1 and KO1) were elevated in the regenerants obtained from MSN+BAP (1.0 mg L-1)+GA3 (1.0 mg L-1).”
t0“Citrus jambhiri Lush.”
Claim · model-written summary
Regenerants from MSN+BAP+GA3 had elevated GA2OX1 and KO1 expression.
- Taxon
- Citrus jambhiri Lush.Citrus jambhirit0
- Treatment
- MSN+BAP (1.0 mg L-1)+GA3 (1.0 mg L-1)e0
- Gene
- GA2OX1 and KO1e0
- Outcome
- expressions of growth regulating factor genes (GRF1 and GRF5) and GA3 signaling genese0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expressions of growth regulating factor genes (GRF1 and GRF5) and GA3 signaling genes (GA2OX1 and KO1) were elevated in the regenerants obtained from MSN+BAP (1.0 mg L-1)+GA3 (1.0 mg L-1).”
t0“Citrus jambhiri Lush.”
Claim · model-written summary
Roots grown on half-strength MSN plus IAA had high auxin-regulating gene expression.
- Taxon
- Citrus jambhiri Lush.Citrus jambhirit0
- Treatment
- ½ MSN+IAA 1.0 mg L-1e0
- Gene
- auxin regulating genese0
- Outcome
- expressions of auxin regulating genese0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expressions of auxin regulating genes were high in roots obtained in ½ MSN+IAA 1.0 mg L-1.”
t0“Citrus jambhiri Lush.”
Claim · model-written summary
Indexing detected no Huanglongbing bacterium amplicons in regenerants.
- Taxon
- Citrus jambhiri Lush.Citrus jambhirit0
- Interactor
- Huanglongbing bacteriume0pathogen
- Outcome
- no amplicons detected for Huanglongbing bacteriume0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, indexing of the regenerants confirmed that there was no amplicons detected for Huanglongbing bacterium and Citrus tristeza virus.”
t0“Citrus jambhiri Lush.”
Claim · model-written summary
Indexing detected no Citrus tristeza virus amplicons in regenerants.
- Taxon
- Citrus jambhiri Lush.Citrus jambhirit0
- Interactor
- Citrus tristeza virusCitrus tristeza viruse0pathogen
- Outcome
- no amplicons detected for Huanglongbing bacterium and Citrus tristeza viruse0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, indexing of the regenerants confirmed that there was no amplicons detected for Huanglongbing bacterium and Citrus tristeza virus.”
t0“Citrus jambhiri Lush.”
Claim · model-written summary
RAPD and ISSR markers detected no polymorphic bands among regenerated plants.
- Taxon
- Citrus jambhiri Lush.Citrus jambhirit0
- Outcome
- no polymorphic bands amongst the regenerated plantse0
- Comparator
- regenerated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers detected no polymorphic bands amongst the regenerated plants.”
t0“Citrus jambhiri Lush.”
The same questions in other papers
indole-3-acetic acid · growth · Citrus jambhiri (rough lemon)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- positive
in vitro · growth · Citrus jambhiri (rough lemon)Positive and negative results
6 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.