Abstract
The effect of Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of growth hormones on direct regeneration from one month old epicotyl segments of in vitro grown rough lemon (Citrus jambhiri Lush.) seedlings was studied. The earliest bud induction in 7.5 days, highest bud induction frequency (98.50%), percent regeneration(90.53) were obtained on MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1) with an average number of 12.50 buds per explants. The epicotyls segments with proliferated buds were transferred to elongation media in order to improve the recovery of normal shoots. Maximum number of elongated shoots (8.50) was obtained on MS medium having BAP (0.5mglit-1) + Gibberellic Acid (GA3)(1.0 mglit-1).These elongated shoots were then rooted on MS medium containing Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1) with highest rooting percentage(96%) and root number(5.0). Early (10.10 days) rooting was observed in MS medium supplemented with NAA1.0 mglit-1 + IBA0.5 mglit-1.The plantlet survival was 98.52%, when plantlets were transferred to plastic pots containing a mixture of garden soil and vermiculite (1:1). The hardened plants were successfully established in the soil. The present study developed protocol which can be reliably used for in vitro regeneration of rough lemon and for gene transfer studies in rough lemon, especially to induce salinity and Phytophthora tolerance.
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Claims 9
Claim · model-written summary
BAP at 1 mg/L on MS medium gave the highest bud induction frequency (98.50%) from epicotyl segments
- Taxon
- rough lemon (Citrus jambhiri Lush.)
.jpg?width=60)
Citrus jambhirit0 - Organ / stage
- epicotyl segmentse0
- Treatment
- MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1)e1
- Outcome
- bud induction frequencye1
- Direction
- higheste1
- Value
- 98.50%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis”
e0“direct regeneration from one month old epicotyl segments of in vitro grown rough lemon (Citrus jambhiri Lush.) seedlings was studied”
e1“The earliest bud induction in 7.5 days, highest bud induction frequency (98.50%), percent regeneration(90.53) were obtained on MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1) with an average number of 12.50 buds per explants”
Claim · model-written summary
BAP at 1 mg/L on MS medium gave the earliest bud induction, at 7.5 days
- Taxon
- rough lemon (Citrus jambhiri Lush.)Citrus jambhirit0
- Treatment
- MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1)e1
- Outcome
- earliest bud inductione1
- Direction
- earlieste1
- Value
- 7.5 dayse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis”
e1“The earliest bud induction in 7.5 days, highest bud induction frequency (98.50%), percent regeneration(90.53) were obtained on MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1) with an average number of 12.50 buds per explants”
Claim · model-written summary
BAP at 1 mg/L on MS medium gave an average of 12.50 buds per explant
- Taxon
- rough lemon (Citrus jambhiri Lush.)Citrus jambhirit0
- Treatment
- MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1)e1
- Outcome
- buds per explantse1
- Value
- 12.50e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis”
e1“The earliest bud induction in 7.5 days, highest bud induction frequency (98.50%), percent regeneration(90.53) were obtained on MS medium supplemented with 6-Benzylaminopurine (BAP) (1mglit-1) with an average number of 12.50 buds per explants”
Claim · model-written summary
MS medium with BAP 0.5 mg/L and GA3 1.0 mg/L gave the maximum number of elongated shoots (8.50)
- Taxon
- rough lemon (Citrus jambhiri Lush.)Citrus jambhirit0
- Treatment
- MS medium having BAP (0.5mglit-1) + Gibberellic Acid (GA3)(1.0 mglit-1)e2
- Outcome
- number of elongated shootse2
- Direction
- Maximume2
- Value
- 8.50e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis”
e2“Maximum number of elongated shoots (8.50) was obtained on MS medium having BAP (0.5mglit-1) + Gibberellic Acid (GA3)(1.0 mglit-1)”
Claim · model-written summary
IBA 0.1 mg/L plus IAA 0.5 mg/L on MS medium gave the highest rooting percentage (96%)
- Taxon
- rough lemon (Citrus jambhiri Lush.)Citrus jambhirit0
- Treatment
- Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1)e3
- Outcome
- rooting percentagee3
- Direction
- higheste3
- Value
- 96%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis”
e3“These elongated shoots were then rooted on MS medium containing Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1) with highest rooting percentage(96%) and root number(5.0)”
Claim · model-written summary
IBA 0.1 mg/L plus IAA 0.5 mg/L on MS medium gave the highest root number (5.0)
- Taxon
- rough lemon (Citrus jambhiri Lush.)Citrus jambhirit0
- Treatment
- Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1)e3
- Outcome
- root numbere3
- Direction
- higheste3
- Value
- 5.0e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis”
e3“These elongated shoots were then rooted on MS medium containing Indole-3-butyric acid (IBA) (0.1mglit-1) + Indole-3-aceticacid(IAA)(0.5mglit-1) with highest rooting percentage(96%) and root number(5.0)”
Claim · model-written summary
NAA plus IBA on MS medium gave early rooting, at 10.10 days
- Taxon
- rough lemon (Citrus jambhiri Lush.)Citrus jambhirit0
- Treatment
- MS medium supplemented with NAA1.0 mglit-1 + IBA0.5 mglit-1e4
- Outcome
- rootinge4
- Direction
- Earlye4
- Value
- 10.10 dayse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis”
e4“Early (10.10 days) rooting was observed in MS medium supplemented with NAA1.0 mglit-1 + IBA0.5 mglit-1”
Claim · model-written summary
Transplanted plantlets had 98.52% survival in plastic pots of garden soil and vermiculite (1:1)
- Taxon
- rough lemon (Citrus jambhiri Lush.)Citrus jambhirit0
- Outcome
- plantlet survivale5
- Value
- 98.52%e5
- Setting
- plastic pots containing a mixture of garden soil and vermiculite (1:1)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis”
e5“The plantlet survival was 98.52%, when plantlets were transferred to plastic pots containing a mixture of garden soil and vermiculite (1:1)”
Claim · model-written summary
The developed protocol can be reliably used for in vitro regeneration of rough lemon
- Taxon
- rough lemon (Citrus jambhiri Lush.)Citrus jambhirit0
- Outcome
- in vitro regeneration of rough lemone6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“In vitro plant regeneration in rough lemon (Citrus jambhiri Lush.) through epicotyl segments by direct shoot organogenesis”
e6“The present study developed protocol which can be reliably used for in vitro regeneration of rough lemon and for gene transfer studies in rough lemon, especially to induce salinity and Phytophthora tolerance”
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See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.