Abstract
As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source to various biotic and abiotic stresses. However, little has been reported on the mechanism of drought tolerance in S. spontaneum. Herein, the physiological changes of S. spontaneum GXS87-16 at three water-deficit levels (mild, moderate, and severe) and after re-watering during the elongation stage were investigated. RNA sequencing was utilized for global transcriptome profiling of GXS87-16 under severe drought and re-watered conditions. There were significant alterations in the physiological parameters of GXS87-16 in response to drought stress and then recovered differently after re-watering. A total of 1569 differentially expressed genes (DEGs) associated with water stress and re-watering were identified. Notably, the majority of the DEGs were induced by stress. GO functional annotations and KEGG pathway analysis assigned the DEGs to 47 GO categories and 93 pathway categories. The pathway categories were involved in various processes, such as RNA transport, mRNA surveillance, plant hormone signal transduction, and plant-pathogen interaction. The reliability of the RNA-seq results was confirmed by qRT-PCR. This study shed light on the regulatory processes of drought tolerance in S. spontaneum and identifies useful genes for genetic improvement of drought tolerance in sugarcane.
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Claims 9
Claim · model-written summary
Water deficit and re-watering significantly altered physiological parameters of S. spontaneum GXS87-16.
- Taxon
- Saccharum spontaneum
Saccharum spontaneume0 - Material
- GXS87-16e1
- Treatment
- drought stresse1
- Outcome
- physiological parameterse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“There were significant alterations in the physiological parameters of GXS87-16 in response to drought stress and then recovered differently after re-watering”
e0“As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source”
Claim · model-written summary
A total of 1569 differentially expressed genes were associated with water stress and re-watering.
- Taxon
- Saccharum spontaneumSaccharum spontaneume0
- Treatment
- water stress and re-wateringe2
- Outcome
- differentially expressed genes (DEGs)e2
- Value
- 1569e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“A total of 1569 differentially expressed genes (DEGs) associated with water stress and re-watering were identified”
e0“As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source”
Claim · model-written summary
The majority of differentially expressed genes were induced by stress.
- Taxon
- Saccharum spontaneumSaccharum spontaneume0
- Treatment
- stresse3
- Outcome
- DEGse3
- Direction
- inducede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Notably, the majority of the DEGs were induced by stress”
e0“As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source”
Claim · model-written summary
Differentially expressed genes were assigned to 47 GO categories.
- Taxon
- Saccharum spontaneumSaccharum spontaneume0
- Outcome
- DEGse4
- Value
- 47 GO categoriese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“GO functional annotations and KEGG pathway analysis assigned the DEGs to 47 GO categories and 93 pathway categories”
e0“As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source”
Claim · model-written summary
Differentially expressed genes were assigned to 93 pathway categories.
- Taxon
- Saccharum spontaneumSaccharum spontaneume0
- Outcome
- DEGse4
- Value
- 93 pathway categoriese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“GO functional annotations and KEGG pathway analysis assigned the DEGs to 47 GO categories and 93 pathway categories”
e0“As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source”
Claim · model-written summary
Pathway categories involved RNA transport, mRNA surveillance and plant hormone signal transduction.
- Taxon
- Saccharum spontaneumSaccharum spontaneume0
- Outcome
- pathway categoriese5
- Qualifier
- RNA transport, mRNA surveillance, plant hormone signal transductione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The pathway categories were involved in various processes, such as RNA transport, mRNA surveillance, plant hormone signal transduction, and plant-pathogen interaction”
e0“As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source”
Claim · model-written summary
Pathway categories involved plant-pathogen interaction.
- Taxon
- Saccharum spontaneumSaccharum spontaneume0
- Outcome
- pathway categoriese5
- Qualifier
- plant-pathogen interactione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The pathway categories were involved in various processes, such as RNA transport, mRNA surveillance, plant hormone signal transduction, and plant-pathogen interaction”
e0“As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source”
Claim · model-written summary
qRT-PCR confirmed the reliability of the RNA-seq results.
Verbatim
e6“The reliability of the RNA-seq results was confirmed by qRT-PCR”
Claim · model-written summary
The study identifies useful genes for genetic improvement of drought tolerance in sugarcane.
Verbatim
e7“identifies useful genes for genetic improvement of drought tolerance in sugarcane”