Abstract
Plants have evolved a series of sophisticated defense mechanisms to help them from harm. Ethylene Response Factor (ERF) plays pivotal roles in plant immune reactions, however, its underlying mechanism in maize with a defensive function to Exserohilum turcicum ( E. turcicum ) remains poorly understood. Here, we isolated and characterized a novel ERF transcription factor, designated ZmERF061 , from maize. Phylogenetic analysis revealed that ZmERF061 is a member of B3 group in the ERF family. qRT-PCR assays showed that the expression of ZmERF061 is significantly induced by E. turcicum inoculation and hormone treatments with salicylic acid (SA) and methyl jasmonate (MeJA). ZmERF061 was proved to function as a nucleus-localized transcription activator and specifically bind to the GCC-box element. zmerf061 mutant lines resulted in enhanced susceptibility to E. turcicum via decreasing the expression of ZmPR10.1 and ZmPR10.2 and the activity of antioxidant defense system. zmerf061 mutant lines increased the expression of the SA signaling-related gene ZmPR1a and decreased the expression of the jasmonic acid (JA) signaling-related gene ZmLox1 after infection with E. turcicum . In addition, ZmERF061 could interact with ZmMPK6-1. These results suggested that ZmERF061 plays an important role in response to E. turcicum and may be useful in genetic engineering breeding.
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Claims 9
Claim · model-written summary
ZmERF061 was identified as a maize ERF transcription factor in the B3 group.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel ERF transcription factor, designated ZmERF061 , from maize. Phylogenetic analysis revealed that ZmERF061 is a member of B3 group in the ERF family”
Claim · model-written summary
E. turcicum inoculation significantly induced maize ZmERF061 expression.
- Treatment
- E. turcicum inoculatione0
- Interactor
- E. turcicum
Exserohilum turcicume0pathogen - Gene
- ZmERF061e0
- Outcome
- expression of ZmERF061e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of ZmERF061 is significantly induced by E. turcicum inoculation”
t0“Maize”
Claim · model-written summary
Salicylic acid and methyl jasmonate treatments significantly induced ZmERF061 expression.
- Treatment
- hormone treatments with salicylic acid (SA) and methyl jasmonate (MeJA)e0
- Gene
- ZmERF061e0
- Outcome
- expression of ZmERF061e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of ZmERF061 is significantly induced by E. turcicum inoculation and hormone treatments with salicylic acid (SA) and methyl jasmonate (MeJA)”
t0“Maize”
Claim · model-written summary
ZmERF061 functioned as a nuclear transcription activator and bound GCC-box.
- Gene
- ZmERF061e0
- Outcome
- nucleus-localized transcription activator and specifically bind to the GCC-box elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmERF061 was proved to function as a nucleus-localized transcription activator and specifically bind to the GCC-box element”
t0“Maize”
Claim · model-written summary
zmerf061 mutant lines were more susceptible to E. turcicum.
- Material
- zmerf061 mutant linese0
- Interactor
- E. turcicumExserohilum turcicume0pathogen
- Outcome
- susceptibility to E. turcicume0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“zmerf061 mutant lines resulted in enhanced susceptibility to E. turcicum”
t0“Maize”
Claim · model-written summary
zmerf061 mutant lines had decreased ZmPR10.1 and ZmPR10.2 expression and antioxidant defense activity.
- Material
- zmerf061 mutant linese0
- Interactor
- E. turcicumExserohilum turcicume0pathogen
- Gene
- ZmPR10.1 and ZmPR10.2e0
- Outcome
- expression of ZmPR10.1 and ZmPR10.2 and the activity of antioxidant defense systeme0
- Direction
- decreasinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“zmerf061 mutant lines resulted in enhanced susceptibility to E. turcicum via decreasing the expression of ZmPR10.1 and ZmPR10.2 and the activity of antioxidant defense system”
t0“Maize”
Claim · model-written summary
After E. turcicum infection, zmerf061 mutants increased ZmPR1a expression.
- Material
- zmerf061 mutant linese0
- Interactor
- E. turcicumExserohilum turcicume0pathogen
- Gene
- ZmPR1ae0
- Outcome
- expression of the SA signaling-related gene ZmPR1ae0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“zmerf061 mutant lines increased the expression of the SA signaling-related gene ZmPR1a and decreased the expression of the jasmonic acid (JA) signaling-related gene ZmLox1 after infection with E. turcicum”
t0“Maize”
Claim · model-written summary
After E. turcicum infection, zmerf061 mutants decreased ZmLox1 expression.
- Material
- zmerf061 mutant linese0
- Interactor
- E. turcicumExserohilum turcicume0pathogen
- Gene
- ZmLox1e0
- Outcome
- expression of the jasmonic acid (JA) signaling-related gene ZmLox1e0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“zmerf061 mutant lines increased the expression of the SA signaling-related gene ZmPR1a and decreased the expression of the jasmonic acid (JA) signaling-related gene ZmLox1 after infection with E. turcicum”
t0“Maize”
Claim · model-written summary
ZmERF061 interacted with ZmMPK6-1.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmERF061 could interact with ZmMPK6-1”
t0“Maize”
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