Abstract
We previously demonstrated that exogenous trehalose 6-phosphate (T6P) treatment stabilized WRINKLED1 (WRI1), a master transcriptional regulator of fatty acid (FA) synthesis and increased total FA content in Brassica napus ( B. napus ) embryo suspension cell culture. Here, we explore Arabidopsis lines heterologously expressing the Escherichia coli T6P synthase (otsA) or T6P phosphatase (otsB) to refine our understanding regarding the role of T6P in regulating fatty acid synthesis both in seeds and vegetative tissues. Arabidopsis 35S: otsA transgenic seeds showed an increase of 13% in fatty acid content compared to those of wild type (WT), while seeds of 35: otsB transgenic seeds showed a reduction of 12% in fatty acid content compared to WT. Expression of otsB significantly reduced the level of WRI1 and expression of its target genes in developing seeds. Like Arabidopsis seeds constitutively expressing otsA, transient expression of otsA in Nicotiana benthamiana leaves resulted in strongly elevated levels of T6P. This was accompanied by an increase of 29% in de novo fatty acid synthesis rate, a 2.3-fold increase in triacylglycerol (TAG) and a 20% increase in total fatty acid content relative to empty vector (EV) controls. Taken together, these data support the heterologous expression of otsA as an approach to increasing TAG accumulation in plant seeds and vegetative tissues.
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Claims 8
Claim · model-written summary
Arabidopsis otsA seeds had 13% more fatty acid content than wild type.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- 35S: otsA transgenic seedse0
- Treatment
- 35S: otsAe0
- Gene
- otsAe0
- Outcome
- fatty acid contente0
- Direction
- increasee0
- Value
- 13%e0
- Comparator
- wild type (WT)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis 35S: otsA transgenic seeds showed an increase of 13% in fatty acid content compared to those of wild type (WT)”
Claim · model-written summary
Arabidopsis otsB seeds had 12% less fatty acid content than wild type.
- Taxon
- ArabidopsisArabidopsise1
- Material
- 35: otsB transgenic seedse0
- Treatment
- 35: otsBe0
- Gene
- otsBe0
- Outcome
- fatty acid contente0
- Direction
- reductione0
- Value
- 12%e0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seeds of 35: otsB transgenic seeds showed a reduction of 12% in fatty acid content compared to WT”
e1“Arabidopsis 35S: otsA transgenic seeds”
Claim · model-written summary
otsB expression significantly reduced WRI1 level and its target gene expression in developing seeds.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Expression of otsBe0
- Gene
- WRI1e0
- Outcome
- level of WRI1 and expression of its target genese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of otsB significantly reduced the level of WRI1 and expression of its target genes in developing seeds.”
e1“Arabidopsis 35S: otsA transgenic seeds”
Claim · model-written summary
Transient otsA expression in Nicotiana leaves strongly elevated T6P levels.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- transient expression of otsAe0
- Gene
- otsAe0
- Outcome
- levels of T6Pe0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient expression of otsA in Nicotiana benthamiana leaves resulted in strongly elevated levels of T6P”
Claim · model-written summary
Transient otsA expression increased de novo fatty acid synthesis rate by 29% in Nicotiana leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- transient expression of otsAe1
- Outcome
- de novo fatty acid synthesis ratee0
- Direction
- increasee0
- Value
- 29%e0
- Comparator
- empty vector (EV) controlse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This was accompanied by an increase of 29% in de novo fatty acid synthesis rate”
e1“transient expression of otsA in Nicotiana benthamiana leaves”
e2“relative to empty vector (EV) controls”
Claim · model-written summary
Transient otsA expression increased triacylglycerol 2.3-fold in Nicotiana leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- transient expression of otsAe1
- Outcome
- triacylglycerol (TAG)e0
- Direction
- increasee0
- Value
- 2.3-folde0
- Comparator
- empty vector (EV) controlse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 2.3-fold increase in triacylglycerol (TAG)”
e1“transient expression of otsA in Nicotiana benthamiana leaves”
e2“relative to empty vector (EV) controls”
Claim · model-written summary
Transient otsA expression increased total fatty acid content by 20% in Nicotiana leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- transient expression of otsAe1
- Outcome
- total fatty acid contente0
- Direction
- increasee0
- Value
- 20%e0
- Comparator
- empty vector (EV) controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 20% increase in total fatty acid content relative to empty vector (EV) controls”
e1“transient expression of otsA in Nicotiana benthamiana leaves”
Claim · model-written summary
The authors support heterologous otsA expression to increase TAG in plant seeds and vegetative tissues.
Verbatim
e0“these data support the heterologous expression of otsA as an approach to increasing TAG accumulation in plant seeds and vegetative tissues.”
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See all 46 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.