Abstract
LRXs (leucine-rich repeat extensins) are chimeric cell wall proteins containing an N-terminal leucine-rich repeat (LRR) and a C-terminal extensin domain. Increasing evidences suggest that LRXs family genes play important roles in pollen germination and pollen tube growth in Arabidopsis thaliana. However, the functions of rice (Oryza sativa L.) LRX genes in pollen development remain poorly understood. Bioinformatics analysis showed that the rice LRX gene family consist of eight members, namely OsPEX3, OsLRX3 and OsLRX5 located on chromosome 1, OsLRX1, OsLRX3, OsLRX2,OsPEX1 and OsPEX2 located on chromosome 2, 5, 6, 11 and 12, respectively. The OsPEX1 gene is preferentially expressed in rice anther, suggesting that it may be involved in the regulation of pollen development. Next, we further investigated the role of the OsPEX1 gene in rice by knockdown of its expression using an RNAi approach. The OsPEX1 RNAi transgenic lines showed a significant decrease in seed setting rate (10%~30%) due to pollen sterility. Further quantitative RT-PCR analysis indicated that the OsPEX1 gene was significantly down-regulated in the RNAi transgenic lines. The results indicate that the OsPEX1plays an important role in the regulation of rice pollen development. Further studies on this gene could provide insights on the molecular and genetic mechanisms in this developmental process.
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Claims 5
Claim · model-written summary
The rice LRX family comprises eight members on the stated chromosomes.
- Taxon
- rice
Oryza sativae0 - Gene
- LRXe0
- Outcome
- gene family consist of eight memberse0
- Value
- eight memberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice LRX gene family consist of eight members, namely OsPEX3, OsLRX3 and OsLRX5 located on chromosome 1, OsLRX1, OsLRX3, OsLRX2,OsPEX1 and OsPEX2 located on chromosome 2, 5, 6, 11 and 12, respectively.”
Claim · model-written summary
OsPEX1 was preferentially expressed in rice anthers.
- Taxon
- riceOryza sativae0
- Organ / stage
- anthere0
- Gene
- OsPEX1e0
- Outcome
- preferentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OsPEX1 gene is preferentially expressed in rice anther”
Claim · model-written summary
OsPEX1 RNAi lines had a significant 10%~30% decrease in seed setting rate associated with pollen sterility.
- Taxon
- riceOryza sativat0
- Material
- OsPEX1 RNAi transgenic linese0
- Treatment
- RNAie0
- Gene
- OsPEX1e0
- Outcome
- seed setting ratee0
- Direction
- decreasee0
- Value
- 10%~30%e0
- Qualifier
- pollen sterilitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OsPEX1 RNAi transgenic lines showed a significant decrease in seed setting rate (10%~30%) due to pollen sterility.”
t0“rice”
Claim · model-written summary
OsPEX1 expression was significantly down-regulated in RNAi lines.
- Taxon
- riceOryza sativat0
- Treatment
- RNAi transgenic linese0
- Gene
- OsPEX1e0
- Outcome
- OsPEX1 genee0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OsPEX1 gene was significantly down-regulated in the RNAi transgenic lines.”
t0“rice”
Claim · model-written summary
The authors infer that OsPEX1 regulates rice pollen development.
- Taxon
- riceOryza sativae0
- Gene
- OsPEX1e0
- Outcome
- rice pollen developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results indicate that the OsPEX1plays an important role in the regulation of rice pollen development.”