Abstract
RNA silencing is a fundamental plant defence and gene control mechanism in plants that are directed by 20-24 nucleotide(nt) small interfering RNA(siRNA) and microRNA(miRNA). Infection of plants with viral pathogens or transformation of plants with RNA interference(RNAi) constructs is usually associated with high levels of exogenous siRNAs, but it is unclear if these siRNAs interfere with endogenous small RNA pathways and hence affect plant development. Here we provide evidence that viral satellite RNA(satRNA) infection does not affect siRNA and miRNA biogenesis or plant growth despite the extremely high level of satRNA-derived siRNAs. We generated transgenic Nicotiana benthamiana plants that no longer develop the specifi c yellowing symptoms generally associated with infection by Cucumber mosaic virus(CMV) Y-satellite RNA(Y-Sat). We then used these plants to show that CMV Y-Sat infection did not cause any visible phenotypic changes in comparison to uninfected plants, despite the presence of high-level Y-Sat siRNAs. Furthermore, we showed that the accumulation of hairpin RNA(hpRNA)-derived siRNAs or miRNAs, and the level of siRNA-directed transgene silencing, are not signifi cantly affected by CMV Y-Sat infection. Taken together, our results suggest that the high levels of exogenous siRNAs associated with viral infection or RNAi-inducing transgenes do not saturate the endogenous RNA silencing machineries and have no signifi cant impact on normal plant development.
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Claims 7
Claim · model-written summary
Transgenic Nicotiana benthamiana plants lacked the usual yellowing symptoms of Y-Sat infection.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Material
- transgenic Nicotiana benthamiana plantse0
- Interactor
- Cucumber mosaic virus(CMV)
cucumber mosaic cucumoviruse0pathogen - Outcome
- no longer develop the specifi c yellowing symptomse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Nicotiana benthamiana plants that no longer develop the specifi c yellowing symptoms generally associated with infection by Cucumber mosaic virus(CMV) Y-satellite RNA(Y-Sat)”
t0“High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants”
Claim · model-written summary
Y-Sat infection caused no visible phenotype in those plants relative to uninfected plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- CMV Y-Sat infectione0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Outcome
- visible phenotypic changese0
- Comparator
- uninfected plantse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CMV Y-Sat infection did not cause any visible phenotypic changes in comparison to uninfected plants”
t0“High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants”
e1“transgenic Nicotiana benthamiana plants”
Claim · model-written summary
Y-Sat infection did not significantly affect hairpin RNA-derived siRNA accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- CMV Y-Sat infectione0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Outcome
- accumulation of hairpin RNA(hpRNA)-derived siRNAse0
- Direction
- not signifi cantly affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of hairpin RNA(hpRNA)-derived siRNAs or miRNAs, and the level of siRNA-directed transgene silencing, are not signifi cantly affected by CMV Y-Sat infection”
t0“High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants”
e1“transgenic Nicotiana benthamiana plants”
Claim · model-written summary
Y-Sat infection did not significantly affect miRNA accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- CMV Y-Sat infectione0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Outcome
- miRNAse0
- Direction
- not signifi cantly affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of hairpin RNA(hpRNA)-derived siRNAs or miRNAs, and the level of siRNA-directed transgene silencing, are not signifi cantly affected by CMV Y-Sat infection”
t0“High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants”
e1“transgenic Nicotiana benthamiana plants”
Claim · model-written summary
Y-Sat infection did not significantly affect siRNA-directed transgene silencing.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- CMV Y-Sat infectione0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Outcome
- level of siRNA-directed transgene silencinge0
- Direction
- not signifi cantly affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of hairpin RNA(hpRNA)-derived siRNAs or miRNAs, and the level of siRNA-directed transgene silencing, are not signifi cantly affected by CMV Y-Sat infection”
t0“High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants”
e1“transgenic Nicotiana benthamiana plants”
Claim · model-written summary
The authors infer that abundant exogenous siRNAs do not saturate endogenous RNA silencing machinery.
- Taxon
- plantsViridiplantaet0
- Outcome
- do not saturate the endogenous RNA silencing machineriese0
- Qualifier
- high levels of exogenous siRNAse0
Verbatim
e0“high levels of exogenous siRNAs associated with viral infection or RNAi-inducing transgenes do not saturate the endogenous RNA silencing machineries”
t0“High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants”
Claim · model-written summary
The authors infer no significant effect of abundant exogenous siRNAs on normal plant development.
- Taxon
- plantsViridiplantaet0
- Treatment
- high levels of exogenous siRNAse0
- Outcome
- normal plant developmente0
- Direction
- no signifi cant impacte0
Verbatim
e0“high levels of exogenous siRNAs associated with viral infection or RNAi-inducing transgenes do not saturate the endogenous RNA silencing machineries and have no signifi cant impact on normal plant development”
t0“High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants”
The same questions in other papers
Cucumis sativus · cucumber mosaic cucumovirus · Nicotiana benthamianaResults agree
4 papers in the same direction as this paper
- no effect
- negative
- negative
1 paper more in this group
See all 5 papers →infection · cucumber mosaic cucumovirus · Nicotiana benthamianaResults agree
3 papers in the same direction as this paper
- no effect
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.