Abstract
The GST (glutathione S-transferase) and GST+CAT1 (catalase 1) of Suaeda salsa were introduced into a low temperature-sensitive rice cultivar (Oryza sativa cv. Zhonghua No.11) by Agrobacterium tumefaciens-mediated transformation under the control of cauliflower mosaic virus (CaMV) 35S promoter, and the transformed calli and plantlets were screened on Murashige and Skoog (1962) medium supplemented with hygromycin 25 microg/mL and cefotaxime 300 microg/mL. The putative primary transformants (T(0) generation) were acclimatized at 26 degrees C /22 degrees C in a greenhouse for 7 d, and then transplanted to the field, where they grew up to maturity under outdoor conditions. 25 and 14 independent transgenic lines of T(1) generation carrying the GST and GST+CAT1 genes, respectively, were identified by PCR amplification. Transgene expression was monitored by RNA-blot hybridization using total RNA samples from leaf tissues. To investigate whether expressing the Suaeda salsa GST and GST+CAT1 in transgenic rice increased low temperature stress tolerance, the T(4) 14-day-old transgenic and non-transgenic rice seedlings were transferred to a low temperature (day 7 degrees C/night 4 degrees C) growth chamber for 3-6 d. The experimental data showed that expressing the Suaeda salsa GST and GST+CAT1 enhanced low temperature stress resistance in transgenic rice seedlings. When treated with low temperature, both GST and CAT activity increased in the transformants with the time of temperature treatment. These transgenic rice plant seedlings exhibited a higher level of photosynthetic capacity than those of the non-transgenic control seedlings under low temperature treatment. Whereas, there were lower H(2)O(2) and MDA (malondialdehyde) content, and relative electrolyte leakage through the plasma membrane was also lower in transgenic rice seedlings than in the parent line under low temperature condition. The results also indicated that GST+CAT1 co-expression conferred greater level of low temperature stress tolerance to the transformed rice plants compared to the single GST transformed plants.
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Claims 6
Claim · model-written summary
Expressing Suaeda salsa GST enhanced low-temperature stress resistance in transgenic rice seedlings compared with non-transgenic controls.
- Taxon
- rice
Oryza sativae1 - Treatment
- low temperature (day 7 degrees C/night 4 degrees C)e2
- Gene
- Suaeda salsa GSTe1
- Outcome
- low temperature stress resistancee1
- Direction
- enhancede1
- Comparator
- non-transgenic control seedlingse3
- Setting
- growth chambere2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“expressing the Suaeda salsa GST and GST+CAT1 enhanced low temperature stress resistance in transgenic rice seedlings”
e2“the T(4) 14-day-old transgenic and non-transgenic rice seedlings were transferred to a low temperature (day 7 degrees C/night 4 degrees C) growth chamber for 3-6 d”
e3“These transgenic rice plant seedlings exhibited a higher level of photosynthetic capacity than those of the non-transgenic control seedlings under low temperature treatment”
Claim · model-written summary
GST+CAT1 co-expression conferred greater low-temperature stress tolerance in rice than single GST expression.
- Taxon
- riceOryza sativae4
- Treatment
- low temperaturee4
- Gene
- GST+CAT1e4
- Outcome
- low temperature stress tolerancee4
- Direction
- greatere4
- Comparator
- single GST transformed plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“GST+CAT1 co-expression conferred greater level of low temperature stress tolerance to the transformed rice plants compared to the single GST transformed plants”
Claim · model-written summary
GST and CAT activity increased in transgenic rice with the time of low-temperature treatment.
- Taxon
- riceOryza sativae1
- Treatment
- low temperaturee5
- Outcome
- GST and CAT activitye5
- Direction
- increasede5
- Qualifier
- with the time of temperature treatmente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“expressing the Suaeda salsa GST and GST+CAT1 enhanced low temperature stress resistance in transgenic rice seedlings”
e5“When treated with low temperature, both GST and CAT activity increased in the transformants with the time of temperature treatment”
Claim · model-written summary
Transgenic rice seedlings had higher photosynthetic capacity than non-transgenic controls under low temperature.
- Taxon
- riceOryza sativae3
- Treatment
- low temperature treatmente3
- Outcome
- photosynthetic capacitye3
- Direction
- highere3
- Comparator
- non-transgenic control seedlingse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“These transgenic rice plant seedlings exhibited a higher level of photosynthetic capacity than those of the non-transgenic control seedlings under low temperature treatment”
Claim · model-written summary
Transgenic rice had lower H2O2 and MDA content than the parent line under low temperature.
- Taxon
- riceOryza sativae6
- Treatment
- low temperature conditione6
- Outcome
- H(2)O(2) and MDA (malondialdehyde) contente6
- Direction
- lowere6
- Comparator
- parent linee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“there were lower H(2)O(2) and MDA (malondialdehyde) content, and relative electrolyte leakage through the plasma membrane was also lower in transgenic rice seedlings than in the parent line under low temperature condition”
Claim · model-written summary
Transgenic rice had lower relative electrolyte leakage through the plasma membrane than the parent line under low temperature.
- Taxon
- riceOryza sativae6
- Treatment
- low temperature conditione6
- Outcome
- relative electrolyte leakage through the plasma membranee6
- Direction
- lowere6
- Comparator
- parent linee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“there were lower H(2)O(2) and MDA (malondialdehyde) content, and relative electrolyte leakage through the plasma membrane was also lower in transgenic rice seedlings than in the parent line under low temperature condition”
The same questions in other papers
cold · tolerance: abiotic · Oryza sativa (rice)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.