Abstract
Capsicum annuum Bukang is a resistant variety to Cucumber mosaic virus isolate-P0 (CMV-P0), CMV-P1 can overcome the CMV resistance of Bukang due to mutations in Helicase (Hel) domain of CMV RNA1. To identify host factors involved in CMV-P1 infection, a yeast two-hybrid system derived from C. annuum Bukang cDNA library was used. A total of 156 potential clones interacting with the CMV-P1 RNA helicase domain were isolated. These clones were confirmed by β-galactosidase filter lift assay, PCR screening and sequence analysis. Then, we narrowed the ten candidate host genes which are related to virus infection, replication or virus movement. To elucidate functions of these candidate genes, each gene was silenced by virus induced gene silencing in Nicotiana benthamiana. The silenced plants were then inoculated with green fluorescent protein (GFP) tagged CMV-P1. Virus accumulations in silenced plants were assessed by monitoring GFP fluorescence and enzyme-linked immunosorbent assay (ELISA). Among ten genes, silencing of formate dehydrogenase (FDH) or calreticulin-3 (CRT3) resulted in weak GFP signals of CMV-P1 in the inoculated or upper leaves. These results suggested that FDH and CRT3 are essential for CMV infection in plants. The importance of FDH and CRT3 in CMV-P1 accumulation was also validated by the accumulation level of CMV coat protein confirmed by ELISA. Altogether, these results demonstrate that FDH and CRT3 are required for CMV-P1 infection in plants.
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Claims 4
Claim · model-written summary
Silencing of formate dehydrogenase (FDH) or calreticulin-3 (CRT3) resulted in weak GFP signals of CMV-P1 in inoculated or upper leaves.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- virus induced gene silencinge0
- Interactor
- CMV-P1e1pathogen
- Gene
- formate dehydrogenase (FDH) or calreticulin-3 (CRT3)e1
- Outcome
- GFP signals of CMV-P1e1
- Direction
- weake1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“each gene was silenced by virus induced gene silencing in Nicotiana benthamiana”
e1“Among ten genes, silencing of formate dehydrogenase (FDH) or calreticulin-3 (CRT3) resulted in weak GFP signals of CMV-P1 in the inoculated or upper leaves”
Claim · model-written summary
FDH and CRT3 silencing affected the accumulation of CMV coat protein measured by ELISA.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- FDH and CRT3e2
- Interactor
- CMV-P1e2pathogen
- Outcome
- CMV coat proteine2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“each gene was silenced by virus induced gene silencing in Nicotiana benthamiana”
e2“The importance of FDH and CRT3 in CMV-P1 accumulation was also validated by the accumulation level of CMV coat protein confirmed by ELISA”
Claim · model-written summary
A total of 156 pepper clones interacting with the CMV-P1 RNA helicase domain were isolated.
- Taxon
- Capsicum annuum
Capsicum annuume0 - Interactor
- CMV-P1e3pathogen
- Outcome
- potential clonese3
- Value
- 156e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Capsicum annuum Bukang is a resistant variety to Cucumber mosaic virus isolate-P0”
e3“A total of 156 potential clones interacting with the CMV-P1 RNA helicase domain were isolated”
Claim · model-written summary
Ten candidate pepper host genes related to virus infection, replication or movement were narrowed down.
- Taxon
- Capsicum annuumCapsicum annuume0
- Outcome
- candidate host genese4
- Value
- tene4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Capsicum annuum Bukang is a resistant variety to Cucumber mosaic virus isolate-P0”
e4“we narrowed the ten candidate host genes which are related to virus infection, replication or virus movement”
The same questions in other papers
loss of function · quality: protein · Nicotiana benthamianaPositive and negative results
3 papers in the same direction as this paper · 10 in the opposite direction
- negative
- negative
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.