Abstract
The present investigation was undertaken to standardize the embryo culture technique for plant regeneration from immature embryos in wheat-rye crosses. Embryo rescue was tried to obtain the hybrid plants. At 17-19 days after pollination, the frequency of embryo development showed that wheat cvs. Hope and Highbury were incompatible with both rye cvs. (Russian rye and Rye 8461), while UP 2338 and WH 896 were incompatible with Russian rye only. The compatibility was the highest in the cross of Chinese Spring (CS) with Russian rye. The embryos were cultured on two media combinations, viz., Taira and Larter’s modified Norstog’s (TL1 medium) and TL1 medium supplemented with 0.5 mg/l of naphthalene acetic acid (NAA) and 1.0 mg/l Kinetin (6-furfuryl amino purine) (TL2 medium) following Taira and Larter (1978). Maximum embryo survival (85.0 %) was observed in CS and CS/Hope 5B crosses with Russian rye, when cultured on Taira and Larter’s modified Norstog’s medium. Hence, it was found that Taira and Larter’s modified Norstog’s medium was the best for plant regeneration studies in wheat-rye crosses.
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Claims 8
Claim · model-written summary
Hope was incompatible with both rye cvs. (Russian rye and Rye 8461).
- Taxon
- wheat
Triticum aestivume0 - Material
- Hopee0
- Outcome
- incompatible with both rye cvs. (Russian rye and Rye 8461)e0
- Qualifier
- 17-19 days after pollinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 17-19 days after pollination, the frequency of embryo development showed that wheat cvs. Hope and Highbury were incompatible with both rye cvs. (Russian rye and Rye 8461), while UP 2338 and WH 896 were incompatible with Russian rye only.”
Claim · model-written summary
Highbury was incompatible with both rye cvs. (Russian rye and Rye 8461).
- Taxon
- wheatTriticum aestivume0
- Material
- Highburye0
- Outcome
- incompatible with both rye cvs. (Russian rye and Rye 8461)e0
- Qualifier
- 17-19 days after pollinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 17-19 days after pollination, the frequency of embryo development showed that wheat cvs. Hope and Highbury were incompatible with both rye cvs. (Russian rye and Rye 8461), while UP 2338 and WH 896 were incompatible with Russian rye only.”
Claim · model-written summary
UP 2338 was incompatible with Russian rye.
- Taxon
- wheatTriticum aestivume0
- Material
- UP 2338e0
- Outcome
- incompatible with Russian ryee0
- Qualifier
- 17-19 days after pollinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 17-19 days after pollination, the frequency of embryo development showed that wheat cvs. Hope and Highbury were incompatible with both rye cvs. (Russian rye and Rye 8461), while UP 2338 and WH 896 were incompatible with Russian rye only.”
Claim · model-written summary
WH 896 was incompatible with Russian rye.
- Taxon
- wheatTriticum aestivume0
- Material
- WH 896e0
- Outcome
- incompatible with Russian ryee0
- Qualifier
- 17-19 days after pollinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 17-19 days after pollination, the frequency of embryo development showed that wheat cvs. Hope and Highbury were incompatible with both rye cvs. (Russian rye and Rye 8461), while UP 2338 and WH 896 were incompatible with Russian rye only.”
Claim · model-written summary
Chinese Spring crossed with Russian rye had the highest compatibility.
- Taxon
- WheatTriticum aestivumt0
- Material
- Chinese Spring (CS)e0
- Outcome
- compatibilitye0
- Direction
- higheste0
- Comparator
- Russian ryee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The compatibility was the highest in the cross of Chinese Spring (CS) with Russian rye.”
t0“Standardization of Regeneration Protocol in Wheat-Rye Crosses from Immature Embryos”
Claim · model-written summary
CS crosses with Russian rye had maximum embryo survival of 85.0% on modified Norstog medium.
- Taxon
- WheatTriticum aestivumt0
- Material
- CSe0
- Treatment
- Taira and Larter’s modified Norstog’s mediume0
- Outcome
- embryo survivale0
- Direction
- Maximume0
- Value
- 85.0 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum embryo survival (85.0 %) was observed in CS and CS/Hope 5B crosses with Russian rye, when cultured on Taira and Larter’s modified Norstog’s medium.”
t0“Standardization of Regeneration Protocol in Wheat-Rye Crosses from Immature Embryos”
Claim · model-written summary
CS/Hope 5B crosses with Russian rye had maximum embryo survival of 85.0% on modified Norstog medium.
- Taxon
- WheatTriticum aestivumt0
- Material
- CS/Hope 5Be0
- Treatment
- Taira and Larter’s modified Norstog’s mediume0
- Outcome
- embryo survivale0
- Direction
- Maximume0
- Value
- 85.0 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum embryo survival (85.0 %) was observed in CS and CS/Hope 5B crosses with Russian rye, when cultured on Taira and Larter’s modified Norstog’s medium.”
t0“Standardization of Regeneration Protocol in Wheat-Rye Crosses from Immature Embryos”
Claim · model-written summary
Modified Norstog medium was best for wheat-rye regeneration studies.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Taira and Larter’s modified Norstog’s mediume0
- Outcome
- best for plant regeneration studiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hence, it was found that Taira and Larter’s modified Norstog’s medium was the best for plant regeneration studies in wheat-rye crosses.”
The same questions in other papers
in vitro · survival · Triticum aestivum (wheat)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.