Abstract
Hypersensitive response (HR)-like cell death is an important mechanism that mediates the plant response to pathogens. In our previous study, we reported that NbHIR3s regulate HR-like cell death and basal immunity. However, the host genes involved in HR have rarely been studied. Here, we used transcriptome sequencing to identify Niben101Scf02063g02012.1 , an HR-like lesion inducing protein (HRLI) in Nicotiana benthamiana that was significantly reduced by turnip mosaic virus (TuMV). HRLIs are uncharacterized proteins which may regulate the HR process. We identified all six HRLIs in N. benthamiana and functionally analyzed Niben101Scf02063g02012.1 , named NbHRLI4 , in response to TuMV. Silencing of NbHRLI4 increased TuMV accumulation, while overexpression of NbHRLI4 conferred resistance to TuMV. Transient overexpression of NbHRLI4 caused cell death with an increase in the expression of salicylic acid (SA) pathway genes but led to less cell death level and weaker immunity in plants expressing NahG . Thus, we have characterized NbHRLI4 as an inducer of cell death and an antiviral regulator of TuMV infection in a SA-mediated manner.
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Claims 8
Claim · model-written summary
Turnip mosaic virus significantly reduced Niben101Scf02063g02012.1 in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Interactor
- turnip mosaic virus
Turnip mosaic viruse0pathogen - Gene
- Niben101Scf02063g02012.1e0
- Outcome
- Niben101Scf02063g02012.1e0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Niben101Scf02063g02012.1 , an HR-like lesion inducing protein (HRLI) in Nicotiana benthamiana that was significantly reduced by turnip mosaic virus (TuMV)”
Claim · model-written summary
Six HRLIs were identified in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Gene
- HRLIse0
- Outcome
- identified all six HRLIse0
- Value
- sixe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified all six HRLIs in N. benthamiana”
Claim · model-written summary
Silencing NbHRLI4 increased TuMV accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Silencing of NbHRLI4e0
- Interactor
- TuMVTurnip mosaic viruse0pathogen
- Gene
- NbHRLI4e0
- Outcome
- TuMV accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of NbHRLI4 increased TuMV accumulation”
t0“Nicotiana benthamiana”
Claim · model-written summary
Overexpression of NbHRLI4 conferred resistance to TuMV.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- overexpression of NbHRLI4e0
- Interactor
- TuMVTurnip mosaic viruse0pathogen
- Gene
- NbHRLI4e0
- Outcome
- resistance to TuMVe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of NbHRLI4 conferred resistance to TuMV”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient NbHRLI4 overexpression caused cell death.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Transient overexpression of NbHRLI4e0
- Gene
- NbHRLI4e0
- Outcome
- cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of NbHRLI4 caused cell death”
t0“Nicotiana benthamiana”
Claim · model-written summary
Transient NbHRLI4 overexpression increased expression of salicylic acid pathway genes.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Transient overexpression of NbHRLI4e0
- Gene
- salicylic acid (SA) pathway genese0
- Outcome
- expression of salicylic acid (SA) pathway genese0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of NbHRLI4 caused cell death with an increase in the expression of salicylic acid (SA) pathway genes”
t0“Nicotiana benthamiana”
Claim · model-written summary
Plants expressing NahG had less cell death under transient NbHRLI4 overexpression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Transient overexpression of NbHRLI4e1
- Gene
- NahGe0
- Outcome
- cell death levele0
- Direction
- lesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“led to less cell death level and weaker immunity in plants expressing NahG”
e1“Transient overexpression of NbHRLI4”
t0“Nicotiana benthamiana”
Claim · model-written summary
Plants expressing NahG had weaker immunity under transient NbHRLI4 overexpression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Transient overexpression of NbHRLI4e1
- Gene
- NahGe0
- Outcome
- immunitye0
- Direction
- weakere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“led to less cell death level and weaker immunity in plants expressing NahG”
e1“Transient overexpression of NbHRLI4”
t0“Nicotiana benthamiana”
The same questions in other papers
loss of function · Turnip mosaic virus · Nicotiana benthamianaPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.