Abstract
BACKGROUND: CRISPR/Cas systems confer immunity against invading nucleic acids and phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 type VI-A ribonuclease capable of targeting and cleaving single-stranded RNA (ssRNA) molecules of the phage genome. Here, we employ CRISPR/Cas13a to engineer interference with an RNA virus, Turnip Mosaic Virus (TuMV), in plants. RESULTS: CRISPR/Cas13a produces interference against green fluorescent protein (GFP)-expressing TuMV in transient assays and stable overexpression lines of Nicotiana benthamiana. CRISPR RNA (crRNAs) targeting the HC-Pro and GFP sequences exhibit better interference than those targeting other regions such as coat protein (CP) sequence. Cas13a can also process pre-crRNAs into functional crRNAs. CONCLUSIONS: Our data indicate that CRISPR/Cas13a can be used for engineering interference against RNA viruses, providing a potential novel mechanism for RNA-guided immunity against RNA viruses and for other RNA manipulations in plants.
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Claims 3
Claim · model-written summary
CRISPR/Cas13a interfered with GFP-expressing TuMV in transient assays and stable Nicotiana benthamiana lines.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- CRISPR/Cas13ae0
- Interactor
- TuMV
Turnip mosaic viruse0pathogen - Outcome
- interference against green fluorescent protein (GFP)-expressing TuMVe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR/Cas13a produces interference against green fluorescent protein (GFP)-expressing TuMV in transient assays and stable overexpression lines of Nicotiana benthamiana”
Claim · model-written summary
crRNAs targeting HC-Pro and GFP interfered better than those targeting CP.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- crRNAs) targeting the HC-Pro and GFP sequencese0
- Outcome
- interferencee0
- Direction
- bettere0
- Comparator
- coat protein (CP) sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR RNA (crRNAs) targeting the HC-Pro and GFP sequences exhibit better interference than those targeting other regions such as coat protein (CP) sequence”
e1“stable overexpression lines of Nicotiana benthamiana”
Claim · model-written summary
Cas13a processed pre-crRNAs into functional crRNAs.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- Cas13ae0
- Outcome
- process pre-crRNAs into functional crRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cas13a can also process pre-crRNAs into functional crRNAs”
e1“stable overexpression lines of Nicotiana benthamiana”
The same questions in other papers
loss of function · Turnip mosaic virus · Nicotiana benthamianaPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.