Abstract
Jasmonic acid (JA) is a crucial hormone in plant antiviral immunity. Increasing evidence shows that viruses counter this host immune response by interfering with JA biosynthesis and signaling. However, the mechanism by which viruses affect JA biosynthesis is still largely unexplored. Here, we show that a highly conserved chloroplast protein cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV). Its silencing facilitated TuMV infection. Furthermore, cpSRP54 interacted with allene oxide cyclases (AOCs), key JA biosynthesis enzymes, and was responsible for delivering AOCs onto the thylakoid membrane (TM). Interestingly, TuMV P1 protein interacted with cpSRP54 and mediated its degradation via the 26S proteosome and autophagy pathways. The results suggest that TuMV has evolved a strategy, through the inhibition of cpSRP54 and its delivery of AOCs to the TM, to suppress JA biosynthesis and enhance viral infection. Interaction between cpSRP54 and AOCs was shown to be conserved in Arabidopsis and rice, while cpSRP54 also interacted with, and was degraded by, pepper mild mottle virus (PMMoV) 126 kDa protein and potato virus X (PVX) p25 protein, indicating that suppression of cpSRP54 may be a common mechanism used by viruses to counter the antiviral JA pathway.
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Claims 11
Claim · model-written summary
cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- infected by turnip mosaic viruse0
- Interactor
- turnip mosaic virus
Turnip mosaic viruse0pathogen - Gene
- cpSRP54e0
- Outcome
- cpSRP54e0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV)”
Claim · model-written summary
Silencing cpSRP54 facilitated TuMV infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- silencinge1
- Interactor
- TuMVTurnip mosaic viruse1pathogen
- Gene
- cpSRP54e1
- Outcome
- TuMV infectione1
- Direction
- facilitatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV). Its silencing facilitated TuMV infection”
Claim · model-written summary
cpSRP54 interacted with allene oxide cyclases (AOCs), key JA biosynthesis enzymes.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Gene
- cpSRP54e2
- Outcome
- interacted with allene oxide cyclases (AOCs)e2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV)”
e2“cpSRP54 interacted with allene oxide cyclases (AOCs), key JA biosynthesis enzymes, and was responsible for delivering AOCs onto the thylakoid membrane (TM)”
Claim · model-written summary
cpSRP54 is responsible for delivering AOCs onto the thylakoid membrane.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Gene
- cpSRP54e2
- Outcome
- delivering AOCs onto the thylakoid membranee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV)”
e2“cpSRP54 interacted with allene oxide cyclases (AOCs), key JA biosynthesis enzymes, and was responsible for delivering AOCs onto the thylakoid membrane (TM)”
Claim · model-written summary
TuMV P1 protein mediated the degradation of cpSRP54 via the 26S proteasome and autophagy pathways.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Interactor
- TuMVTurnip mosaic viruse3pathogen
- Gene
- TuMV P1 proteine3
- Outcome
- degradatione3
- Qualifier
- via the 26S proteosome and autophagy pathwayse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV)”
e3“TuMV P1 protein interacted with cpSRP54 and mediated its degradation via the 26S proteosome and autophagy pathways”
Claim · model-written summary
TuMV suppresses JA biosynthesis and enhances viral infection through inhibition of cpSRP54 and its delivery of AOCs to the thylakoid membrane.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Interactor
- TuMVTurnip mosaic viruse4pathogen
- Gene
- cpSRP54e4
- Outcome
- JA biosynthesise4
- Direction
- suppresse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV)”
e4“The results suggest that TuMV has evolved a strategy, through the inhibition of cpSRP54 and its delivery of AOCs to the TM, to suppress JA biosynthesis and enhance viral infection”
Claim · model-written summary
The interaction between cpSRP54 and AOCs is conserved in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise5 - Gene
- cpSRP54e5
- Outcome
- Interaction between cpSRP54 and AOCse5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Interaction between cpSRP54 and AOCs was shown to be conserved in Arabidopsis and rice”
Claim · model-written summary
The interaction between cpSRP54 and AOCs is conserved in rice.
- Taxon
- rice
Oryza sativae5 - Gene
- cpSRP54e5
- Outcome
- Interaction between cpSRP54 and AOCse5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“Interaction between cpSRP54 and AOCs was shown to be conserved in Arabidopsis and rice”
Claim · model-written summary
Pepper mild mottle virus 126 kDa protein interacted with and degraded cpSRP54.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Interactor
- pepper mild mottle virus
Pepper mild mottle viruse6pathogen - Gene
- cpSRP54e6
- Outcome
- degradede6
- Qualifier
- 126 kDa proteine6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV)”
e6“cpSRP54 also interacted with, and was degraded by, pepper mild mottle virus (PMMoV) 126 kDa protein and potato virus X (PVX) p25 protein”
Claim · model-written summary
Potato virus X p25 protein interacted with and degraded cpSRP54.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Interactor
- potato virus X (PVX)Potato virus Xe6pathogen
- Gene
- cpSRP54e6
- Outcome
- degradede6
- Qualifier
- p25 proteine6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV)”
e6“cpSRP54 also interacted with, and was degraded by, pepper mild mottle virus (PMMoV) 126 kDa protein and potato virus X (PVX) p25 protein”
Claim · model-written summary
Suppression of cpSRP54 may be a common viral mechanism for countering the antiviral JA pathway.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Gene
- cpSRP54e7
- Outcome
- antiviral JA pathwaye7
- Qualifier
- common mechanisme7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cpSRP54 was downregulated in Nicotiana benthamiana infected by turnip mosaic virus (TuMV)”
e7“suppression of cpSRP54 may be a common mechanism used by viruses to counter the antiviral JA pathway”
The same questions in other papers
Brassica rapa subsp. rapa · Turnip mosaic virus · Nicotiana benthamianaResults agree
2 papers in the same direction as this paper
- negative
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infection · Turnip mosaic virus · Nicotiana benthamianaPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
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loss of function · Turnip mosaic virus · Nicotiana benthamianaPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.