Paper · 2020 · IOP Conference Series Earth and Environmental Science

Effect of auxin (2,4-D) and cytokinin (BAP) in callus induction of local patchouli plants (Pogostemon cablin Benth.)

R Mayerni; B Satria; DK Wardhani; et al.

4
claims
8
verbatim quotes
23
citations
2
research areas
✓
Open access · cc-by

Abstract

Abstract Patchouli propagates only through vegetative because patchouli has no flowers, so generative propagation is not possible. Traditionally patchouli plants are propagated by using stem cuttings. Alternative methods for vegetative propagation of superior, healthy, and relatively short seedlings can be done through tissue culture techniques, which can reproduce clones with identical genetic in a short time. The success of propagation in vitro is determined by many factors, including growth regulators used. Growth regulators which are often used in tissue culture to initiate callus and increase the production of secondary metabolites (organogenesis) are auxins and cytokinins. The purpose of this study was to determine the best concentration of 2,4-D and BAP in the formation of patchouli callus in vitro. Research methods arranged in a completely randomized design and ex-plants derived from local patchouli plants namely Situak. The results showed that in many concentrations of 2,4 D and BAP to callus induction. Callus formed from giving concentrations in combination without 2,4 D and 1,0 mg/l BAP, concentrations of 1,0 mg/l 2,4-D and 1,0 mg/l BAP, concentrations of 1,5 mg/l 2,4-D and 1,0 mg/l BAP, and concentrations of 2,0 mg/l 2,4-D and 1,0 mg/l BAP.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Callus formed without 2,4 D and with 1,0 mg/l BAP.

Taxon
Pogostemon cablin Benth.Pogostemon cablint0
Treatment
without 2,4 D and 1,0 mg/l BAPe0
Outcome
Callus formede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Callus formed from giving concentrations in combination without 2,4 D and 1,0 mg/l BAP”

✓ located verbatim in the source · abstract, characters 1028–1114 · show in text ↑
t0

“Pogostemon cablin Benth.”

✓ located verbatim in the source · title, characters 91–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Callus formed with 1,0 mg/l 2,4-D and 1,0 mg/l BAP.

Taxon
Pogostemon cablin Benth.Pogostemon cablint0
Treatment
1,0 mg/l 2,4-D and 1,0 mg/l BAPe0
Outcome
Callus formede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Callus formed from giving concentrations in combination without 2,4 D and 1,0 mg/l BAP, concentrations of 1,0 mg/l 2,4-D and 1,0 mg/l BAP”

✓ located verbatim in the source · abstract, characters 1028–1165 · show in text ↑
t0

“Pogostemon cablin Benth.”

✓ located verbatim in the source · title, characters 91–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Callus formed with 1,5 mg/l 2,4-D and 1,0 mg/l BAP.

Taxon
Pogostemon cablin Benth.Pogostemon cablint0
Treatment
1,5 mg/l 2,4-D and 1,0 mg/l BAPe0
Outcome
Callus formede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Callus formed from giving concentrations in combination without 2,4 D and 1,0 mg/l BAP, concentrations of 1,0 mg/l 2,4-D and 1,0 mg/l BAP, concentrations of 1,5 mg/l 2,4-D and 1,0 mg/l BAP”

✓ located verbatim in the source · abstract, characters 1028–1216 · show in text ↑
t0

“Pogostemon cablin Benth.”

✓ located verbatim in the source · title, characters 91–115 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Callus formed with 2,0 mg/l 2,4-D and 1,0 mg/l BAP.

Taxon
Pogostemon cablin Benth.Pogostemon cablint0
Treatment
2,0 mg/l 2,4-D and 1,0 mg/l BAPe0
Outcome
Callus formede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pogostemon cablin
Pogostemon cablin · TaxonNo machine-readable author provided. Valérie75 assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Callus formed from giving concentrations in combination without 2,4 D and 1,0 mg/l BAP, concentrations of 1,0 mg/l 2,4-D and 1,0 mg/l BAP, concentrations of 1,5 mg/l 2,4-D and 1,0 mg/l BAP, and concentrations of 2,0 mg/l 2,4-D and 1,0 mg/l BAP.”

✓ located verbatim in the source · abstract, characters 1028–1272 · show in text ↑
t0

“Pogostemon cablin Benth.”

✓ located verbatim in the source · title, characters 91–115 · show in text ↑

2,4-D · growth · Pogostemon cablin (patchouli)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

N-benzyladenine · growth · Pogostemon cablin (patchouli)Results agree

11 papers: 11 positive, 0 negative or no effect

10 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.