Abstract
Abstract Indonesia is a potential producer of patchouli oil, patchouli oil is in great demand, but supply has not kept pace. The scarcity of high-quality seeds contributes to the low patchouli yield. Tissue culture is an alternate technique that may quickly multiply high-quality seeds. This in vitro research set out to establish the optimal concentrations of 2,4-D and BAP for inducing callus formation on patchouli leaves. The study was conducted from September 2023 to May 2024 at Syiah Kuala University’s Plant Tissue Culture Laboratory, Department of Agrotechnology, Faculty of Agriculture, Darussalam, Banda Aceh. The study used a RAL design with two components, including the 2,4-D concentration (0 mg L −1 , 0.5 mg L −1 , 1 mg L −1 , and 1.5 mg L −1 ) and the BAP concentration (0 mg L −1 , 0.5 mg L −1 , 1 mg L −1 , and 1.5 mg L −1 ). At the 5% significance level, the DMRT test was applied to 96 experimental units, which were comprised of 16 treatment combinations with 6 replications. Chlorophyll concentration, stomata on the leaves, percentage of live calluses, percentage of calluses formed, duration to callus emergence, color of calluses, and texture of calluses were the variables measured. Although all treatment combinations resulted in 100% live explants, the most effective one was BAP 1 mg L −1 + 2,4-D 1 mg L −1 , which resulted in 100% callus production and an emergence time of 11 HST for the callus.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 3
Claim · model-written summary
Every treatment combination yielded 100% live patchouli explants.
- Taxon
- Patchouli
Pogostemon cablint0 - Treatment
- all treatment combinationse0
- Outcome
- live explantse0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all treatment combinations resulted in 100% live explants”
t0“Patchouli”
Claim · model-written summary
BAP 1 mg L −1 with 2,4-D 1 mg L −1 yielded 100% callus production.
- Taxon
- PatchouliPogostemon cablint0
- Treatment
- BAP 1 mg L −1 + 2,4-D 1 mg L −1e0
- Outcome
- callus productione0
- Direction
- increaseinferrede0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP 1 mg L −1 + 2,4-D 1 mg L −1 , which resulted in 100% callus production”
t0“Patchouli”
Claim · model-written summary
BAP 1 mg L −1 with 2,4-D 1 mg L −1 produced callus after 11 HST.
- Taxon
- PatchouliPogostemon cablint0
- Treatment
- BAP 1 mg L −1 + 2,4-D 1 mg L −1e0
- Outcome
- emergence timee0
- Value
- 11 HSTe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BAP 1 mg L −1 + 2,4-D 1 mg L −1 , which resulted in 100% callus production and an emergence time of 11 HST for the callus.”
t0“Patchouli”
The same questions in other papers
2,4-D · growth · Pogostemon cablin (patchouli)Results agree
2 papers in the same direction as this paper
- positive
- positive
2,4-D · yield · Pogostemon cablin (patchouli)Results agree
2 papers in the same direction as this paper
- positive
- positive
N-benzyladenine · yield · Pogostemon cablin (patchouli)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
N-benzyladenine · growth · Pogostemon cablin (patchouli)Results agree
10 papers in the same direction as this paper
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.