Abstract
Multiple shoots from nodal explants of Pogostemon cablin Benth. MS supple-mented with 0.5 mg/l BAP initiated a mean of 45.66 shoots/nodal explant. Within four weeks of initiation, regenerated multiple shoots attained a height of 3.6 cm. Subsequent transfer of these in vitro derived nodal segments onto MS supplemented with BAP and Kn ( 0.5 mg/l), induced a mean of 62.45 shoots. Higher concentration of either BAP or Kn more than 0.5 mg/l resulted in callus proliferation and showed hyperhydric shoots with morphological abnormalities. Rooting was readily achieved upon transfer of shoots on half strength MS supplemented with 100 mg/l activated charcoal. Rooted shoots, following acclimatization in greenhouse, were successfully transferred to soil with 91% survival. Also shoots regenerated in vitro were directly transplanted to soil and acclimatized. Tissue cultured plants were analyzed for oil content by employing gas chromatography and found that the patterns were similar to mother plants. This confirms the true to type nature of micropropagated plants. Key words: Micropropagation, Direct regeneration, Pogostemon cablin, organogenesis D.O.I. 10.3329/ptcb.v20i1.5971 Plant Tissue Cult. & Biotech. 20(1): 81-89, 2010 (June)
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Claims 8
Claim · model-written summary
Nodal explants produced a mean 45.66 shoots with 0.5 mg/l BAP.
- Taxon
- Pogostemon cablin Benth.
Pogostemon cabline0 - Treatment
- 0.5 mg/l BAPe0
- Outcome
- shoots/nodal explante0
- Value
- 45.66 shoots/nodal explante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Multiple shoots from nodal explants of Pogostemon cablin Benth. MS supple-mented with 0.5 mg/l BAP initiated a mean of 45.66 shoots/nodal explant.”
Claim · model-written summary
Regenerated shoots reached 3.6 cm in four weeks.
- Taxon
- Pogostemon cablin Benth.Pogostemon cablint0
- Outcome
- heighte0
- Value
- 3.6 cme0
- Qualifier
- Within four weeks of initiatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Within four weeks of initiation, regenerated multiple shoots attained a height of 3.6 cm.”
t0“Pogostemon cablin Benth.”
Claim · model-written summary
Transfer to medium containing BAP and Kn induced a mean 62.45 shoots.
- Taxon
- Pogostemon cablin Benth.Pogostemon cablint0
- Treatment
- BAP and Kn ( 0.5 mg/l)e0
- Outcome
- shootse0
- Value
- 62.45 shootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subsequent transfer of these in vitro derived nodal segments onto MS supplemented with BAP and Kn ( 0.5 mg/l), induced a mean of 62.45 shoots.”
t0“Pogostemon cablin Benth.”
Claim · model-written summary
Higher BAP or Kn concentrations caused callus proliferation and hyperhydric abnormal shoots.
- Taxon
- Pogostemon cablin Benth.Pogostemon cablint0
- Treatment
- Higher concentration of either BAP or Kn more than 0.5 mg/le0
- Outcome
- callus proliferation and showed hyperhydric shoots with morphological abnormalitiese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher concentration of either BAP or Kn more than 0.5 mg/l resulted in callus proliferation and showed hyperhydric shoots with morphological abnormalities.”
t0“Pogostemon cablin Benth.”
Claim · model-written summary
Half-strength medium with activated charcoal readily rooted the shoots.
- Taxon
- Pogostemon cablin Benth.Pogostemon cablint0
- Treatment
- half strength MS supplemented with 100 mg/l activated charcoale0
- Outcome
- Rooting was readily achievede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooting was readily achieved upon transfer of shoots on half strength MS supplemented with 100 mg/l activated charcoal.”
t0“Pogostemon cablin Benth.”
Claim · model-written summary
Acclimatized rooted shoots survived soil transfer at 91%.
- Taxon
- Pogostemon cablin Benth.Pogostemon cablint0
- Outcome
- survivale0
- Value
- 91%e0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rooted shoots, following acclimatization in greenhouse, were successfully transferred to soil with 91% survival.”
t0“Pogostemon cablin Benth.”
Claim · model-written summary
In vitro shoots were also transplanted directly to soil and acclimatized.
- Taxon
- Pogostemon cablin Benth.Pogostemon cablint0
- Outcome
- directly transplanted to soil and acclimatizede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Also shoots regenerated in vitro were directly transplanted to soil and acclimatized.”
t0“Pogostemon cablin Benth.”
Claim · model-written summary
Oil patterns of tissue-cultured plants resembled mother plants, supporting true-to-type propagation.
- Taxon
- Pogostemon cablin Benth.Pogostemon cablint0
- Outcome
- oil contente0
- Direction
- similare0
- Comparator
- mother plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tissue cultured plants were analyzed for oil content by employing gas chromatography and found that the patterns were similar to mother plants. This confirms the true to type nature of micropropagated plants.”
t0“Pogostemon cablin Benth.”
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