Abstract
The study of essential oils detection of patchouli leaves (Pogostemon cablin Benth) with combination of 2,4-Dichlorofenoxyacetate and coconut water which produced calli had been done at Departement of Food Crops and Horticulture, Tissue Culture Laboratory, North Sumatra and the Organic Chemistry Laboratory of the Faculty of Mathematics and Natural Sciences, University of Sumatera Utara. The aim of the study was to determine the concentration of the combination of 2.4-D and coconut water which added in MS media for detection essential oils in patchouli calli in vitro. The experiment used factorial Completely Randomized Design (CRD) with two factors. The first factor was coconut water with concentrations of 0, 10 and 20%. The second factor is 2.4-D with a concentrations of 0, 0.5 and 1 ppm.The results showed that the optimum combination of 2,4-D and coconut water was capable to induce calli initiation after 13 DAP (days after planting), 80% of calli production, green color, nonfriable, and calli weight of 0,28 gram. The TLC (Thin Layer Chromatography) result showed that the treatment of 0.5 ppm 2,4-D and 10% coconut water produced the same Rf value as Rf standard (patchouli oil) which was 0.87
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Claims 5
Claim · model-written summary
The optimum 2,4-D and coconut water combination initiated calli after 13 DAP.
- Taxon
- patchouli
Pogostemon cablint0 - Treatment
- combination of 2,4-D and coconut watere0
- Outcome
- calli initiatione0
- Value
- 13 DAPe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimum combination of 2,4-D and coconut water was capable to induce calli initiation after 13 DAP (days after planting)”
t0“patchouli”
Claim · model-written summary
The optimum combination gave 80% calli production.
- Taxon
- patchouliPogostemon cablint0
- Treatment
- combination of 2,4-D and coconut watere0
- Outcome
- calli productione0
- Value
- 80%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimum combination of 2,4-D and coconut water was capable to induce calli initiation after 13 DAP (days after planting), 80% of calli production, green color, nonfriable, and calli weight of 0,28 gram.”
t0“patchouli”
Claim · model-written summary
The resulting calli were green and nonfriable.
- Taxon
- patchouliPogostemon cablint0
- Outcome
- green color, nonfriablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“green color, nonfriable, and calli weight of 0,28 gram.”
t0“patchouli”
Claim · model-written summary
Calli from the optimum combination weighed 0,28 gram.
- Taxon
- patchouliPogostemon cablint0
- Treatment
- combination of 2,4-D and coconut watere0
- Outcome
- calli weighte0
- Value
- 0,28 grame0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimum combination of 2,4-D and coconut water was capable to induce calli initiation after 13 DAP (days after planting), 80% of calli production, green color, nonfriable, and calli weight of 0,28 gram.”
t0“patchouli”
Claim · model-written summary
TLC of calli treated with 0.5 ppm 2,4-D and 10% coconut water matched patchouli oil standard at Rf 0.87.
- Taxon
- patchouliPogostemon cablint0
- Treatment
- 0.5 ppm 2,4-D and 10% coconut watere0
- Outcome
- Rf valuee0
- Value
- 0.87e0
- Comparator
- Rf standard (patchouli oil)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the treatment of 0.5 ppm 2,4-D and 10% coconut water produced the same Rf value as Rf standard (patchouli oil) which was 0.87”
t0“patchouli”
The same questions in other papers
2,4-D · yield · Pogostemon cablin (patchouli)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.