Abstract
Abstract Trichomes function in plant defenses against biotic and abiotic stresses; examination of glabrous lines, which lack trichomes, has revealed key aspects of trichome development and function. Tests of allelism in 51 glabrous rice ( Oryza sativa ) accessions collected worldwide identified OsSPL10 and OsWOX3B as regulators of trichome development in rice. Here, we report that OsSPL10 acts as a transcriptional regulator controlling trichome development. Haplotype and transient expression analyses revealed that variation in the approximately 700‐bp OsSPL10 promoter region is the primary cause of the glabrous phenotype in the indica cultivar WD‐17993. Disruption of OsSPL10 by genome editing decreased leaf trichome density and length in the NIL‐ HL6 background. Plants with genotype OsSPL10 WD‐17993 / HL6 generated by crossing WD‐17993 with NIL‐ HL6 also had fewer trichomes in the glumes. HAIRY LEAF6 ( HL6 ) encodes another transcription factor that regulates trichome initiation and elongation, and OsSPL10 directly binds to the HL6 promoter to regulate its expression. Moreover, the transcript levels of auxin‐related genes, such as OsYUCCA5 and OsPIN‐FORMED1b , were altered in OsSPL10 overexpression and RNAi transgenic lines. Feeding tests using locusts ( Locusta migratoria ) demonstrated that non‐glandular trichomes affect feeding by this herbivore. Our findings provide a molecular framework for trichome development and an ecological perspective on trichome functions.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Allelism tests identified OsSPL10 and OsWOX3B as rice trichome regulators.
- Taxon
- rice
Oryza sativae0 - Gene
- OsSPL10 and OsWOX3Be0
- Outcome
- regulators of trichome developmente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tests of allelism in 51 glabrous rice ( Oryza sativa ) accessions collected worldwide identified OsSPL10 and OsWOX3B as regulators of trichome development in rice.”
Claim · model-written summary
OsSPL10 acts as a transcriptional regulator of trichome development.
- Taxon
- RiceOryza sativat0
- Gene
- OsSPL10e0
- Outcome
- trichome developmente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsSPL10 acts as a transcriptional regulator controlling trichome development.”
t0“Rice”
Claim · model-written summary
Variation in the OsSPL10 promoter primarily causes the glabrous phenotype of WD‐17993.
- Taxon
- RiceOryza sativat0
- Material
- WD‐17993e0
- Gene
- OsSPL10e0
- Outcome
- glabrous phenotypee0
- Value
- approximately 700‐bpe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“variation in the approximately 700‐bp OsSPL10 promoter region is the primary cause of the glabrous phenotype in the indica cultivar WD‐17993”
t0“Rice”
Claim · model-written summary
Genome editing disruption of OsSPL10 decreased leaf trichome density and length in NIL‐ HL6.
- Taxon
- RiceOryza sativat0
- Material
- NIL‐ HL6e0
- Treatment
- Disruption of OsSPL10 by genome editinge0
- Gene
- OsSPL10e0
- Outcome
- leaf trichome density and lengthe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of OsSPL10 by genome editing decreased leaf trichome density and length in the NIL‐ HL6 background.”
t0“Rice”
Claim · model-written summary
OsSPL10 WD‐17993 / HL6 plants had fewer glume trichomes.
- Taxon
- RiceOryza sativat0
- Material
- OsSPL10 WD‐17993 / HL6e0
- Outcome
- trichomes in the glumese0
- Direction
- fewere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants with genotype OsSPL10 WD‐17993 / HL6 generated by crossing WD‐17993 with NIL‐ HL6 also had fewer trichomes in the glumes.”
t0“Rice”
Claim · model-written summary
HL6 encodes a transcription factor regulating trichome initiation and elongation.
- Taxon
- RiceOryza sativat0
- Gene
- HL6e0
- Outcome
- trichome initiation and elongatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HAIRY LEAF6 ( HL6 ) encodes another transcription factor that regulates trichome initiation and elongation”
t0“Rice”
Claim · model-written summary
OsSPL10 directly binds the HL6 promoter and regulates its expression.
- Taxon
- RiceOryza sativat0
- Gene
- OsSPL10e0
- Outcome
- HL6 promotere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsSPL10 directly binds to the HL6 promoter to regulate its expression.”
t0“Rice”
Claim · model-written summary
Auxin-related transcript levels changed in OsSPL10 overexpression and RNAi lines.
- Taxon
- RiceOryza sativat0
- Treatment
- OsSPL10 overexpression and RNAie0
- Gene
- OsYUCCA5 and OsPIN‐FORMED1be0
- Outcome
- transcript levels of auxin‐related genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcript levels of auxin‐related genes, such as OsYUCCA5 and OsPIN‐FORMED1b , were altered in OsSPL10 overexpression and RNAi transgenic lines.”
t0“Rice”
Claim · model-written summary
Feeding assays showed that nonglandular trichomes affect locust feeding.
- Taxon
- RiceOryza sativat0
- Organ / stage
- non‐glandular trichomese0
- Interactor
- Locusta migratoria
Locusta migratoriae0herbivore - Outcome
- feeding by this herbivoree0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Feeding tests using locusts ( Locusta migratoria ) demonstrated that non‐glandular trichomes affect feeding by this herbivore.”
t0“Rice”