Paper · 2021 · Asia-Pacific Journal of Molecular Biology and Biotechnology

Isolation screening and molecular characterization of zinc solubilizing bacteria and their effect on the growth of wheat (Triticum aestivum)

Vandana Nandal; Manu Solanki

4
claims
6
verbatim quotes
4
citations
2
research areas
✓
Open access

Abstract

Wheat (Triticum aestivum) is a major cereal crop grown worldwide. Most of the world population depends on wheat for their nutrient requirement. Zinc (Zn) is one of the most crucial elements required for the development of wheat plant. It is one of the micronutrients required in many biochemical cycles. It has been found that the concentration of Zn is below the required level in the soil and hence it remains deficient in the crops. To ameliorate the deficit, chemical fertilizers are added in the soil, where as biofertilizers are preferred over chemicals in sustainable agriculture. The paper describes the isolation, screening and molecular characterization of the zinc solubilizing bacteria (ZSB) to improve plant growth. A total of 100 soil samples were collected from the rhizospheric soil of wheat plants. ZSB were isolated by dilution plating on Bunt and Rovira media. The 50 isolates were selected and screened for their Zn solubilization. The zinc tolerance of all the isolates varied from 0.5% to 2% of insoluble Zn. Based on the Zn tolerance ability, 15 bacterial isolates were screened for Phosphate solubilization and further analyzed for the synthesis of IAA, NH3, siderophore production and chitinase activity. The three isolates were selected on the basis of the plant growth promoting characteristics for molecular characterization and were found to be homologous to Bacillus cereus, Pseudomonas aeruginosa and Bacillus tropicus. This study documented the establishment and survival of ZSB in the wheat rhizosphere and enhanced plant productivity, thus indicating the potential of isolates as commercial biofertilizers.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

The bacterial isolates tolerated 0.5% to 2% insoluble zinc.

Outcome
zinc tolerance of all the isolatese0
Value
0.5% to 2% of insoluble Zne0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The zinc tolerance of all the isolates varied from 0.5% to 2% of insoluble Zn.”

✓ located verbatim in the source · abstract, characters 952–1030 · show in text ↑
t0

“Wheat (Triticum aestivum)”

✓ located after whitespace/quote normalisation · title, characters 115–140 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Three selected isolates were homologous to Bacillus cereus, Pseudomonas aeruginosa and Bacillus tropicus.

Outcome
homologous to Bacillus cereus, Pseudomonas aeruginosa and Bacillus tropicuse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The three isolates were selected on the basis of the plant growth promoting characteristics for molecular characterization and were found to be homologous to Bacillus cereus, Pseudomonas aeruginosa and Bacillus tropicus.”

✓ located verbatim in the source · abstract, characters 1230–1450 · show in text ↑
t0

“Wheat (Triticum aestivum)”

✓ located after whitespace/quote normalisation · title, characters 115–140 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Zinc-solubilizing bacteria established and survived in the wheat rhizosphere.

Outcome
establishment and survival of ZSB in the wheat rhizospheree0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This study documented the establishment and survival of ZSB in the wheat rhizosphere and enhanced plant productivity”

✓ located verbatim in the source · abstract, characters 1451–1567 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Zinc-solubilizing bacteria enhanced plant productivity.

Treatment
ZSBe0
Outcome
plant productivitye0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This study documented the establishment and survival of ZSB in the wheat rhizosphere and enhanced plant productivity”

✓ located verbatim in the source · abstract, characters 1451–1567 · show in text ↑

zinc atom · yield · Triticum aestivum (wheat)Results differ

511 papers: 456 positive, 55 negative or no effect

455 papers in the same direction as this paper · 49 in the opposite direction · 6 reporting no difference from an alternative

  1. no effect
  2. negative
  3. no effect

507 papers more in this group

See all 511 papers →

inoculation · yield · Triticum aestivum (wheat)Positive and negative results

614 papers: 549 positive, 65 negative or no effect

548 papers in the same direction as this paper · 60 in the opposite direction · 5 reporting no difference from an alternative

  1. positive
  2. negative
  3. negative

610 papers more in this group

See all 614 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.