Abstract
The Arabidopsis AtCRK5 protein kinase is involved in the establishment of the proper auxin gradient in many developmental processes. Among others, the Atcrk5-1 mutant was reported to exhibit a delayed gravitropic response via compromised PIN2-mediated auxin transport at the root tip. Here, we report that this phenotype correlates with lower superoxide anion (O2•−) and hydrogen peroxide (H2O2) levels but a higher nitric oxide (NO) content in the mutant root tips in comparison to the wild type (AtCol-0). The oxidative stress inducer paraquat (PQ) triggering formation of O2•− (and consequently, H2O2) was able to rescue the gravitropic response of Atcrk5-1 roots. The direct application of H2O2 had the same effect. Under gravistimulation, correct auxin distribution was restored (at least partially) by PQ or H2O2 treatment in the mutant root tips. In agreement, the redistribution of the PIN2 auxin efflux carrier was similar in the gravistimulated PQ-treated mutant and untreated wild type roots. It was also found that PQ-treatment decreased the endogenous NO level at the root tip to normal levels. Furthermore, the mutant phenotype could be reverted by direct manipulation of the endogenous NO level using an NO scavenger (cPTIO). The potential involvement of AtCRK5 protein kinase in the control of auxin-ROS-NO-PIN2-auxin regulatory loop is discussed.
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Claims 10
Claim · model-written summary
The delayed mutant phenotype correlated with lower superoxide in root tips than wild type.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- root tipse0
- Outcome
- superoxide anion (O2•−)e0
- Direction
- lowere0
- Comparator
- AtCol-0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this phenotype correlates with lower superoxide anion (O2•−) and hydrogen peroxide (H2O2) levels but a higher nitric oxide (NO) content in the mutant root tips in comparison to the wild type (AtCol-0)”
t0“Arabidopsis”
Claim · model-written summary
The mutant phenotype correlated with lower hydrogen peroxide in root tips.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- root tipse0
- Outcome
- hydrogen peroxide (H2O2) levelse0
- Direction
- lowere0
- Comparator
- AtCol-0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this phenotype correlates with lower superoxide anion (O2•−) and hydrogen peroxide (H2O2) levels but a higher nitric oxide (NO) content in the mutant root tips in comparison to the wild type (AtCol-0)”
t0“Arabidopsis”
Claim · model-written summary
The mutant phenotype correlated with higher nitric oxide content in root tips.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- root tipse0
- Outcome
- nitric oxide (NO) contente0
- Direction
- highere0
- Comparator
- AtCol-0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this phenotype correlates with lower superoxide anion (O2•−) and hydrogen peroxide (H2O2) levels but a higher nitric oxide (NO) content in the mutant root tips in comparison to the wild type (AtCol-0)”
t0“Arabidopsis”
Claim · model-written summary
Paraquat rescued mutant root gravitropism.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Atcrk5-1e0
- Organ / stage
- rootse0
- Treatment
- paraquat (PQ)e0
- Outcome
- gravitropic responsee0
- Direction
- rescuee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The oxidative stress inducer paraquat (PQ) triggering formation of O2•− (and consequently, H2O2) was able to rescue the gravitropic response of Atcrk5-1 roots.”
t0“Arabidopsis”
Claim · model-written summary
Direct hydrogen peroxide application also rescued root gravitropism.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Atcrk5-1e0
- Treatment
- direct application of H2O2e0
- Outcome
- gravitropic responsee0
- Direction
- rescuee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rescue the gravitropic response of Atcrk5-1 roots. The direct application of H2O2 had the same effect.”
t0“Arabidopsis”
Claim · model-written summary
Paraquat partially restored correct auxin distribution under gravistimulation.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- root tipse0
- Treatment
- PQe0
- Outcome
- correct auxin distributione0
- Direction
- restorede0
- Qualifier
- at least partiallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under gravistimulation, correct auxin distribution was restored (at least partially) by PQ or H2O2 treatment in the mutant root tips.”
t0“Arabidopsis”
Claim · model-written summary
Hydrogen peroxide partially restored correct auxin distribution under gravistimulation.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- root tipse0
- Treatment
- H2O2 treatmente0
- Outcome
- correct auxin distributione0
- Direction
- restorede0
- Qualifier
- at least partiallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under gravistimulation, correct auxin distribution was restored (at least partially) by PQ or H2O2 treatment in the mutant root tips.”
t0“Arabidopsis”
Claim · model-written summary
PIN2 redistribution was similar in paraquat treated mutant and untreated wild type roots.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- PQ-treatede0
- Gene
- PIN2e0
- Outcome
- redistributione0
- Direction
- similare0
- Qualifier
- gravistimulatede0
- Comparator
- untreated wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the redistribution of the PIN2 auxin efflux carrier was similar in the gravistimulated PQ-treated mutant and untreated wild type roots”
t0“Arabidopsis”
Claim · model-written summary
Paraquat decreased endogenous nitric oxide at the root tip to normal levels.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- root tipe0
- Treatment
- PQ-treatmente0
- Outcome
- endogenous NO levele0
- Direction
- decreasede0
- Qualifier
- to normal levelse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PQ-treatment decreased the endogenous NO level at the root tip to normal levels”
t0“Arabidopsis”
Claim · model-written summary
The nitric oxide scavenger cPTIO reverted the mutant phenotype.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- NO scavenger (cPTIO)e0
- Outcome
- mutant phenotypee0
- Direction
- revertede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutant phenotype could be reverted by direct manipulation of the endogenous NO level using an NO scavenger (cPTIO)”
t0“Arabidopsis”
The same questions in other papers
hydrogen peroxide · physiology: hormones · ArabidopsisResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.