Abstract
The role of ethylene and hydrogen peroxide (H₂O₂) in the induction of the alternative respiratory pathway (AP) in calluses from wild-type (WT) Arabidopsis and ethylene-insensitive mutant etr1-3 under salt stress was investigated. The capacity and the contribution of the AP to the total respiration were significantly induced by 100 mM sodium chloride (NaCl) in WT calluses but only slightly induced in etr1-3 calluses. Ethylene emission was enhanced in WT calluses under salt stress. Application of 1-aminocyclopropane-1-carboxylic acid (an ethylene precursor) further increased the AP capacity in WT calluses but not in etr1-3 calluses under salt stress. Reduction of ethylene production by aminooxyacetic acid (AOA, an ethylene biosynthesis inhibitor) in WT calluses eliminated the NaCl-induced increase of ethylene emission and inhibited AP induction under salt stress, suggesting that ethylene is required for AP induction. H₂O₂ enhanced ethylene production while ethylene reduced H₂O₂ generation in WT calluses under salt stress. In addition, ethylene and H₂O₂ modulated NaCl-induced alternative oxidase gene (AOX1a) expression and the increase in pyruvate content in WT calluses. Inhibition of the AP by salicylhydroxamic acid in WT calluses under salt stress resulted in severe cellular damage as indicated by the high content of H₂O₂, malondialdehyde and more electrolyte leakage. Taken together, ethylene and H₂O₂ are involved in the salt-induced increase of the AP, which plays an important role in salt tolerance in WT calluses, and ethylene may be acting downstream of H₂O₂.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 15
Claim · model-written summary
NaCl significantly induced AP capacity and contribution in wild-type calluses.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- WTe0
- Treatment
- 100 mM sodium chloride (NaCl)e0
- Outcome
- capacity and the contribution of the AP to the total respiratione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The capacity and the contribution of the AP to the total respiration were significantly induced by 100 mM sodium chloride (NaCl) in WT calluses but only slightly induced in etr1-3 calluses.”
t0“Arabidopsis Calluses”
Claim · model-written summary
NaCl only slightly induced AP measures in etr1-3 calluses.
- Taxon
- ArabidopsisArabidopsist0
- Material
- etr1-3e0
- Treatment
- 100 mM sodium chloride (NaCl)e0
- Outcome
- capacity and the contribution of the AP to the total respiratione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The capacity and the contribution of the AP to the total respiration were significantly induced by 100 mM sodium chloride (NaCl) in WT calluses but only slightly induced in etr1-3 calluses.”
t0“Arabidopsis Calluses”
Claim · model-written summary
Salt stress enhanced ethylene emission in WT calluses.
- Taxon
- ArabidopsisArabidopsist0
- Material
- WTe0
- Treatment
- salt stresse0
- Outcome
- Ethylene emissione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ethylene emission was enhanced in WT calluses under salt stress.”
t0“Arabidopsis Calluses”
Claim · model-written summary
Ethylene precursor increased AP capacity in WT calluses.
- Taxon
- ArabidopsisArabidopsist0
- Material
- WTe0
- Treatment
- 1-aminocyclopropane-1-carboxylic acide0
- Outcome
- AP capacitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of 1-aminocyclopropane-1-carboxylic acid (an ethylene precursor) further increased the AP capacity in WT calluses but not in etr1-3 calluses under salt stress.”
t0“Arabidopsis Calluses”
Claim · model-written summary
Ethylene precursor did not increase AP capacity in etr1-3 calluses.
- Taxon
- ArabidopsisArabidopsist0
- Material
- etr1-3e0
- Treatment
- 1-aminocyclopropane-1-carboxylic acide0
- Outcome
- AP capacitye0
- Direction
- not ine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of 1-aminocyclopropane-1-carboxylic acid (an ethylene precursor) further increased the AP capacity in WT calluses but not in etr1-3 calluses under salt stress.”
t0“Arabidopsis Calluses”
Claim · model-written summary
AOA eliminated NaCl-induced ethylene emission.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- aminooxyacetic acide0
- Outcome
- ethylene emissione0
- Direction
- eliminatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduction of ethylene production by aminooxyacetic acid (AOA, an ethylene biosynthesis inhibitor) in WT calluses eliminated the NaCl-induced increase of ethylene emission and inhibited AP induction under salt stress, suggesting that ethylene is required for AP induction.”
t0“Arabidopsis Calluses”
Claim · model-written summary
AOA inhibited AP induction under salt stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- aminooxyacetic acide0
- Outcome
- AP inductione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduction of ethylene production by aminooxyacetic acid (AOA, an ethylene biosynthesis inhibitor) in WT calluses eliminated the NaCl-induced increase of ethylene emission and inhibited AP induction under salt stress, suggesting that ethylene is required for AP induction.”
t0“Arabidopsis Calluses”
Claim · model-written summary
H₂O₂ enhanced ethylene production.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H₂O₂e0
- Outcome
- ethylene productione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H₂O₂ enhanced ethylene production while ethylene reduced H₂O₂ generation in WT calluses under salt stress.”
t0“Arabidopsis Calluses”
Claim · model-written summary
ethylene reduced H₂O₂ generation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ethylenee0
- Outcome
- H₂O₂ generatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H₂O₂ enhanced ethylene production while ethylene reduced H₂O₂ generation in WT calluses under salt stress.”
t0“Arabidopsis Calluses”
Claim · model-written summary
ethylene modulated alternative oxidase gene (AOX1a) expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ethylenee0
- Outcome
- alternative oxidase gene (AOX1a) expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition, ethylene and H₂O₂ modulated NaCl-induced alternative oxidase gene (AOX1a) expression and the increase in pyruvate content in WT calluses.”
t0“Arabidopsis Calluses”
Claim · model-written summary
H₂O₂ modulated alternative oxidase gene (AOX1a) expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H₂O₂e0
- Outcome
- alternative oxidase gene (AOX1a) expressione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition, ethylene and H₂O₂ modulated NaCl-induced alternative oxidase gene (AOX1a) expression and the increase in pyruvate content in WT calluses.”
t0“Arabidopsis Calluses”
Claim · model-written summary
ethylene modulated pyruvate content.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ethylenee0
- Outcome
- pyruvate contente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition, ethylene and H₂O₂ modulated NaCl-induced alternative oxidase gene (AOX1a) expression and the increase in pyruvate content in WT calluses.”
t0“Arabidopsis Calluses”
Claim · model-written summary
H₂O₂ modulated pyruvate content.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H₂O₂e0
- Outcome
- pyruvate contente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition, ethylene and H₂O₂ modulated NaCl-induced alternative oxidase gene (AOX1a) expression and the increase in pyruvate content in WT calluses.”
t0“Arabidopsis Calluses”
Claim · model-written summary
AP inhibition caused severe cellular damage under salt stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- salicylhydroxamic acide0
- Outcome
- severe cellular damagee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of the AP by salicylhydroxamic acid in WT calluses under salt stress resulted in severe cellular damage as indicated by the high content of H₂O₂, malondialdehyde and more electrolyte leakage.”
t0“Arabidopsis Calluses”
Claim · model-written summary
AP inhibition raised H₂O₂ and malondialdehyde content and electrolyte leakage.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- salicylhydroxamic acide0
- Outcome
- H₂O₂, malondialdehyde and more electrolyte leakagee0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of the AP by salicylhydroxamic acid in WT calluses under salt stress resulted in severe cellular damage as indicated by the high content of H₂O₂, malondialdehyde and more electrolyte leakage.”
t0“Arabidopsis Calluses”
The same questions in other papers
hydrogen peroxide · molecular: metabolites · ArabidopsisPositive and negative results
1 paper in the same direction as this paper · 6 in the opposite direction
- negative
- no effect
- no effect
4 papers more in this group
See all 8 papers →ethene · molecular: metabolites · ArabidopsisPositive and negative results
5 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- no effect
- negative
5 papers more in this group
See all 9 papers →hydrogen peroxide · molecular: expression · ArabidopsisPositive and negative results
12 papers in the same direction as this paper · 4 in the opposite direction
- positive
- no effect
- no effect
13 papers more in this group
See all 17 papers →ethene · molecular: expression · ArabidopsisPositive and negative results
20 papers in the same direction as this paper · 14 in the opposite direction
- negative
- no effect
- no effect
31 papers more in this group
See all 35 papers →ethene · physiology: oxidative · ArabidopsisResults agree
2 papers in the same direction as this paper
- positive
- positive
salicylhydroxamic acid · physiology: oxidative · ArabidopsisResults agree
2 papers in the same direction as this paper
- negative
- negative
hydrogen peroxide · physiology: hormones · ArabidopsisResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.