Abstract
The Arabidopsis CPD gene encodes a cytochrome P450 steroid side-chain hydroxylase (CYP90) that plays an essential role in the biosynthesis of the plant hormone brassinolide. Expression of the CPD gene is confined to cotyledons and leaf primordia in etiolated seedlings and detectable in the adaxial parenchyma of expanding leaves in light-grown plants. Transcription of the CPD gene is not affected by the plant growth factors auxin, ethylene, gibberellin, cytokinin, jasmonic acid and salicylic acid, but is specifically down-regulated by brassinolide in both dark and light. Steady-state mRNA levels of a CPD promoter-driven uidA reporter gene correlate with the expression of resident CPD gene in transgenic plants. Intermediates of the early and late C-6 oxidation pathways of brassinolide, carrying C-22 and C-23 side-chain hydroxyls, efficiently inhibit the activity of the CPD promoter. Repression of CPD transcription by brassinosteroids is sensitive to the protein synthesis inhibitor cycloheximide, indicating a requirement for de novo synthesis of a regulatory factor.
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Claims 6
Claim · model-written summary
CPD expression is confined to cotyledons and leaf primordia of etiolated seedlings.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- cotyledons and leaf primordiae1
- Gene
- CPDe1
- Outcome
- Expression of the CPD genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CPD gene”
e1“Expression of the CPD gene is confined to cotyledons and leaf primordia in etiolated seedlings”
Claim · model-written summary
Auxin, ethylene, gibberellin, cytokinin, jasmonic acid and salicylic acid do not affect CPD transcription.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- auxin, ethylene, gibberellin, cytokinin, jasmonic acid and salicylic acide2
- Gene
- CPDe2
- Outcome
- Transcription of the CPD genee2
- Direction
- no_changeinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CPD gene”
e2“Transcription of the CPD gene is not affected by the plant growth factors auxin, ethylene, gibberellin, cytokinin, jasmonic acid and salicylic acid”
Claim · model-written summary
Brassinolide specifically down-regulates CPD transcription in both dark and light.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- brassinolidee3
- Gene
- CPDe3
- Outcome
- Transcription of the CPD genee3
- Direction
- down-regulatede3
- Qualifier
- in both dark and lighte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CPD gene”
e3“Transcription of the CPD gene is not affected by the plant growth factors auxin, ethylene, gibberellin, cytokinin, jasmonic acid and salicylic acid, but is specifically down-regulated by brassinolide in both dark and light”
Claim · model-written summary
Steady-state mRNA of the CPD promoter-driven uidA reporter correlates with CPD expression in transgenic plants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- transgenic plantse4
- Gene
- CPDe4
- Outcome
- Steady-state mRNA levels of a CPD promoter-driven uidA reporter genee4
- Direction
- correlatee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CPD gene”
e4“Steady-state mRNA levels of a CPD promoter-driven uidA reporter gene correlate with the expression of resident CPD gene in transgenic plants”
Claim · model-written summary
Early and late C-6 oxidation intermediates of brassinolide efficiently inhibit CPD promoter activity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Intermediates of the early and late C-6 oxidation pathways of brassinolidee5
- Gene
- CPDe5
- Outcome
- activity of the CPD promotere5
- Direction
- inhibite5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CPD gene”
e5“Intermediates of the early and late C-6 oxidation pathways of brassinolide, carrying C-22 and C-23 side-chain hydroxyls, efficiently inhibit the activity of the CPD promoter”
Claim · model-written summary
Brassinosteroid repression of CPD transcription is sensitive to cycloheximide, suggesting a requirement for de novo synthesis of a regulatory factor.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- Repression of CPD transcription by brassinosteroidse6
- Qualifier
- sensitive to the protein synthesis inhibitor cycloheximidee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CPD gene”
e6“Repression of CPD transcription by brassinosteroids is sensitive to the protein synthesis inhibitor cycloheximide, indicating a requirement for de novo synthesis of a regulatory factor”
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