Abstract
Summary Protein cysteine thiols are post‐translationally modified under oxidative stress conditions. Illuminated chloroplasts are one of the important sources of hydrogen peroxide (H 2 O 2 ) and are highly sensitive to environmental stimuli, yet a comprehensive view of the oxidation‐sensitive chloroplast proteome is still missing. By targeting the sulfenic acid YAP 1C‐trapping technology to the plastids of light‐grown Arabidopsis cells, we identified 132 putatively sulfenylated plastid proteins upon H 2 O 2 pulse treatment. Almost half of the sulfenylated proteins are enzymes of the amino acid metabolism. Using metabolomics, we observed a reversible decrease in the levels of the amino acids Ala, Asn, Cys, Gln, Glu, His, Ile, Leu, Lys, Phe, Ser, Thr and Val after H 2 O 2 treatment, which is in line with an anticipated decrease in the levels of the glycolysis and tricarboxylic acid metabolites. Through the identification of an organelle‐tailored proteome, we demonstrated that the subcellular targeting of the YAP 1C probe enables us to study in vivo cysteine sulfenylation at the organellar level. All in all, the identification of these oxidation events in plastids revealed that several enzymes of the amino acid metabolism rapidly undergo cysteine oxidation upon oxidative stress.
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Claims 4
Claim · model-written summary
H2O2 pulse treatment identified 132 putatively sulfenylated plastid proteins in Arabidopsis cells.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- H 2 O 2 pulse treatmente0
- Outcome
- putatively sulfenylated plastid proteinse0
- Direction
- identifiede0
- Value
- 132e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis cells”
e0“we identified 132 putatively sulfenylated plastid proteins upon H 2 O 2 pulse treatment”
Claim · model-written summary
Almost half of the sulfenylated proteins are enzymes of amino acid metabolism.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- enzymes of the amino acid metabolisme2
- Value
- Almost halfe2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis cells”
e2“Almost half of the sulfenylated proteins are enzymes of the amino acid metabolism”
Claim · model-written summary
H2O2 treatment reversibly decreased the levels of 13 amino acids in Arabidopsis cells.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- H 2 O 2 treatmente3
- Outcome
- levels of the amino acids Ala, Asn, Cys, Gln, Glu, His, Ile, Leu, Lys, Phe, Ser, Thr and Vale3
- Direction
- decreasee3
- Qualifier
- reversiblee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis cells”
e3“we observed a reversible decrease in the levels of the amino acids Ala, Asn, Cys, Gln, Glu, His, Ile, Leu, Lys, Phe, Ser, Thr and Val after H 2 O 2 treatment”
Claim · model-written summary
The YAP1C probe targeted to plastids enables in vivo study of cysteine sulfenylation at the organellar level.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- in vivo cysteine sulfenylation at the organellar levele4
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Arabidopsis cells”
e4“the subcellular targeting of the YAP 1C probe enables us to study in vivo cysteine sulfenylation at the organellar level”
The same questions in other papers
hydrogen peroxide · molecular: metabolites · ArabidopsisPositive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.