Abstract
Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol with species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing was applied to examine the genetic diversity of X. translucens strains. Strains were assigned to four different sequence types, three of which were previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to X. prunicola and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota. In planta pathogenicity assays carried out on a BLS-susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, whereas non-translucens Xanthomonas sp. strains elicited an atypical symptom of dry necrosis. These findings suggest that local wheat fields are affected by X. translucens pv. undulosa and by a new wheat pathogen within the Xanthomonas genus.
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Claims 9
Claim · model-written summary
Surveys of 61 wheat fields in 2017-2019 yielded 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- Xanthomonas spp.
_-_Howard_F._Schwartz%2C_Colorado_State_University.jpg?width=60)
Xanthomonase0pathogen - Outcome
- regional collection of 63 strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Howard_F._Schwartz%2C_Colorado_State_University.jpg?width=500)
Verbatim
e0“Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp.”
Claim · model-written summary
Real-time PCR with species-specific primers identified 44 of the strains as X. translucens, the causal agent of bacterial leaf streak in wheat.
- Taxon
- wheatTriticum aestivume0
- Interactor
- X. translucens
Xanthomonas translucense0pathogen - Outcome
- identification of 44 strains as X. translucense0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops”
Claim · model-written summary
Multilocus sequence analysis showed these strains were most closely related to X. translucens pv. undulosa, the pathovar most commonly associated with bacterial leaf streak of wheat.
- Taxon
- wheatTriticum aestivume0
- Interactor
- X. translucens pv. undulosaXanthomonas translucens pv. undulosae0pathogen
- Outcome
- most closely related to X. translucens pv. undulosae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat”
Claim · model-written summary
Multilocus sequence typing assigned the X. translucens strains to four sequence types, three of which were previously reported globally.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- XanthomonasXanthomonast0pathogen
- Outcome
- assigned to four different sequence typese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Strains were assigned to four different sequence types, three of which were previously reported globally”
t0“Xanthomonas Species Pathogenic in Wheat in Uruguay”
Claim · model-written summary
Seventeen Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- leavese0
- Interactor
- XanthomonasXanthomonase0pathogen
- Outcome
- 17 Xanthomonas strains not belonging to X. translucens were obtainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves”
Claim · model-written summary
Phylogenetic analysis showed the non-translucens strains are closely related to X. prunicola and cluster with uncharacterized Xanthomonas strains from wheat in Minnesota.
- Taxon
- wheatTriticum aestivume0
- Interactor
- X. prunicolaXanthomonas prunicolae0pathogen
- Outcome
- closely related to X. prunicolae0
- Setting
- Minnesotae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these strains are closely related to X. prunicola and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota”
Claim · model-written summary
In planta assays on a BLS-susceptible wheat cultivar showed X. translucens pv. undulosa strains caused brown necrosis symptoms typical of bacterial leaf streak.
- Taxon
- wheatTriticum aestivume0
- Interactor
- X. translucens pv. undulosaXanthomonas translucens pv. undulosae0pathogen
- Outcome
- brown necrosis symptoms typical of BLSe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pathogenicity assays carried out on a BLS-susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS”
Claim · model-written summary
In the same assays, non-translucens Xanthomonas strains elicited an atypical dry necrosis symptom on the wheat cultivar.
- Taxon
- wheatTriticum aestivume0
- Interactor
- non-translucens Xanthomonas sp.e1pathogen
- Outcome
- atypical symptom of dry necrosise1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“carried out on a BLS-susceptible wheat cultivar”
e1“whereas non-translucens Xanthomonas sp. strains elicited an atypical symptom of dry necrosis”
Claim · model-written summary
The authors conclude that local wheat fields are affected by X. translucens pv. undulosa and by a new wheat pathogen within the Xanthomonas genus.
- Taxon
- wheatTriticum aestivume0
- Interactor
- X. translucens pv. undulosaXanthomonas translucens pv. undulosae0pathogen
- Outcome
- affected by X. translucens pv. undulosa and by a new wheat pathogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“local wheat fields are affected by X. translucens pv. undulosa and by a new wheat pathogen within the Xanthomonas genus”