Claim · model-written summary
Comparative genomics, phylogenomics and the ubcg2 gene set assigned X. translucens pathovars into three genetically and taxonomically distinct clusters.
- Taxon
- X. translucens
Xanthomonas translucense0 - Outcome
- three genetically and taxonomically distinct clusterse0
Verbatim
e0“comparative genomics, phylogenomics, and 81 up-to-date bacterial core gene set (ubcg2) were used to assign the pathovars of X. translucens into three genetically and taxonomically distinct clusters”
Title
James T. Tambong; Renlin Xu; Maria Constanza Fleitas; et al. Phytopathology 2023. doi:10.1094/phyto-01-23-0022-sa
Other claims from this paper (7)
- Observation Whole genome-based digital DNA-DNA hybridization unambiguously differentiated pathovars translucens and undulosa.
- Observation The cluster consisting of graminis, poae, arrhenatheri, phlei and phleipratensis is suggested to be very divergent.
- Method / resource A first pathovar-specific TaqMan real-time PCR tool was developed from whole-genome data for detection of pv. translucens on barley.
- Method / resource Specificity of the TaqMan assay was validated with 62 Xanthomonas and non-Xanthomonas strains and with inoculated and naturally infected barley leaves.
- Method / resource The assay sensitivity of 0.1 pg purified DNA compared favorably with other previously reported real-time PCR assays.
- Method / resource The assay sensitivity of 23 CFUs per reaction (direct culture) compared favorably with other previously reported real-time PCR assays.
- Observation The phylogenomics data suggest that the clusters could constitute novel taxonomic units or new species.