Abstract
Arsenic is one of the most hazardous metalloids in nature, and due to its high water solubility, it is one of the most important causes of pollution. However, silicon reduces the uptake and transport of arsenic in rice. This study investigates the interaction of different arsenic and silicon levels on dry weight, protein content, and concentrations of arsenic and silicon in two different rice shoots and roots of Dular wild-type (DU-WT) and Dular Lsi1-overexpressed (DU-OE) rice. It should be noted that all seedlings were subjected to four different treatments. For RNA-seq and qPCR, the DU-WT genotype was selected as the control and DU-OE as the treatment. With the addition of silicone treatment, dry weight and protein content in the shoots and roots of both rice lines were increased, while the concentration of arsenic in these two organs was decreased. When seedlings were exposed to arsenic treatments, protein content, silicon concentration, and dry weight were decreased in both roots and shoots, while arsenic concentration was increased in both rice genotypes. The RNA-seq in DU-OE showed 5823 differentially expressed genes (DEGs), of which 2604 were up-regulated and 3219 down-regulated. Treatment of rice by arsenic and silicon has changed the expression of genes encoding cytokinin-responsive GATA transcription factor 1, protein IN2-1 homolog B, calcium-binding EGF domain-containing protein, Os01g0369700 protein, probable glutathione S-transferase GSTU1, glutathione S-transferase protein, Os09g0367700 protein, isocitrate dehydrogenase (NADP), and Os08g0522400 protein in the root of DU-OE. The present study's findings showed that in the presence of silicon, the transgenic genotype is much more resistant to arsenic than the wild genotype of Dular rice.
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Claims 8
Claim · model-written summary
Silicon increased shoot and root dry weight in both Dular rice lines.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- shoots and rootse0
- Treatment
- silicone treatmente0
- Outcome
- dry weighte0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With the addition of silicone treatment, dry weight and protein content in the shoots and roots of both rice lines were increased”
Claim · model-written summary
Silicon increased shoot and root protein content in both rice lines.
- Taxon
- riceOryza sativae0
- Treatment
- silicone treatmente0
- Outcome
- protein contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With the addition of silicone treatment, dry weight and protein content in the shoots and roots of both rice lines were increased”
Claim · model-written summary
Silicon decreased arsenic concentration in shoots and roots of both rice lines.
- Taxon
- RiceOryza sativat0
- Treatment
- Silicont0
- Outcome
- concentration of arsenice0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while the concentration of arsenic in these two organs was decreased”
t0“Silicon-Evoked Responses on Arsenic Stress between Different Dular Rice Genotypes”
Claim · model-written summary
Arsenic treatment decreased protein content, silicon concentration and dry weight in roots and shoots of both rice genotypes.
- Taxon
- RiceOryza sativat0
- Organ / stage
- roots and shootse0
- Treatment
- arsenic treatmentse0
- Outcome
- protein content, silicon concentration, and dry weighte0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When seedlings were exposed to arsenic treatments, protein content, silicon concentration, and dry weight were decreased in both roots and shoots”
t0“Rice Genotypes”
Claim · model-written summary
Arsenic treatment increased arsenic concentration in both rice genotypes.
- Taxon
- riceOryza sativae0
- Treatment
- arsenict0
- Outcome
- arsenic concentratione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while arsenic concentration was increased in both rice genotypes”
t0“Arsenic Stress”
Claim · model-written summary
RNA sequencing found 5823 differentially expressed genes in DU-OE, 2604 up-regulated and 3219 down-regulated.
- Taxon
- riceOryza sativat0
- Material
- DU-OEe0
- Outcome
- differentially expressed genese0
- Value
- 5823e0
- Qualifier
- 2604 were up-regulated and 3219 down-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RNA-seq in DU-OE showed 5823 differentially expressed genes (DEGs), of which 2604 were up-regulated and 3219 down-regulated”
t0“Rice”
Claim · model-written summary
Arsenic and silicon treatment altered expression of the listed genes in DU-OE rice roots.
- Taxon
- riceOryza sativae0
- Material
- DU-OEe0
- Organ / stage
- roote0
- Treatment
- arsenic and silicone0
- Outcome
- expression of genese0
- Direction
- changede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of rice by arsenic and silicon has changed the expression of genes encoding cytokinin-responsive GATA transcription factor 1, protein IN2-1 homolog B, calcium-binding EGF domain-containing protein, Os01g0369700 protein, probable glutathione S-transferase GSTU1, glutathione S-transferase protein, Os09g0367700 protein, isocitrate dehydrogenase (NADP), and Os08g0522400 protein in the root of DU-OE”
Claim · model-written summary
With silicon, the transgenic Dular genotype was more resistant to arsenic than the wild genotype.
- Taxon
- riceOryza sativae0
- Outcome
- resistant to arsenice0
- Direction
- much more resistante0
- Qualifier
- in the presence of silicone0
- Comparator
- wild genotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the presence of silicon, the transgenic genotype is much more resistant to arsenic than the wild genotype of Dular rice”
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See all 77 papers →arsenic atom · quality: protein · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.