Abstract
Abstract The vascular wilting caused by Fusarium oxysporum f. sp. dianthi (Fod) is the most relevant disease for carnation cultivation. Understanding the biochemical mechanisms involved in resistance to Fod will allow the development of new disease control strategies. In this research, the levels of some phytohormones such as salicylic acid (SA), methyl salicylate (MeSA), and methyl jasmonate (MeJA) were evaluated in symplast of carnation roots infected with this pathogen. The accumulation of these hormones was then correlated with the expression levels of symplastic peroxidases, enzymes involved in the plant resistance against pathogen during interaction. Our results suggested that pathogen infection causes a differential accumulation of SA, MeSA, and MeJA in a resistant cultivar (i.e. ‘Golem’), being earlier and higher than that observed in a susceptible one (i.e. ‘Solex’). Simultaneously, an increase of guaiacol peroxidase enzymatic activity (GPX) and transcriptional levels of a gene coding for a symplastic peroxidase were presented as part of the resistance response. The positive statistical correlation between the accumulation of SA and MeJA and the expression of peroxidases (GPX activity and mRNA levels) indicates the possible cellular relationship of these phenomena during the activation of the resistance to Fod. Our findings suggested some hormonal signaling mechanisms acting at the roots during the regulation of the biochemical response associated with resistance against Fod.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Infection caused earlier and higher salicylic acid accumulation in resistant Golem than susceptible Solex.
- Taxon
- carnation
Dianthus caryophyllust0 - Material
- ‘Golem’e0
- Treatment
- pathogen infectione0
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporumt0pathogen - Outcome
- SAe0
- Direction
- earlier and highere0
- Comparator
- ‘Solex’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“pathogen infection causes a differential accumulation of SA, MeSA, and MeJA in a resistant cultivar (i.e. ‘Golem’), being earlier and higher than that observed in a susceptible one (i.e. ‘Solex’)”
t0“carnation-Fusarium oxysporum”
Claim · model-written summary
Infection caused earlier and higher methyl salicylate accumulation in resistant Golem than susceptible Solex.
- Taxon
- carnationDianthus caryophyllust0
- Material
- ‘Golem’e0
- Treatment
- pathogen infectione0
- Interactor
- Fusarium oxysporumFusarium oxysporumt0pathogen
- Outcome
- MeSAe0
- Direction
- earlier and highere0
- Comparator
- ‘Solex’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pathogen infection causes a differential accumulation of SA, MeSA, and MeJA in a resistant cultivar (i.e. ‘Golem’), being earlier and higher than that observed in a susceptible one (i.e. ‘Solex’)”
t0“carnation-Fusarium oxysporum”
Claim · model-written summary
Infection caused earlier and higher methyl jasmonate accumulation in resistant Golem than susceptible Solex.
- Taxon
- carnationDianthus caryophyllust0
- Material
- ‘Golem’e0
- Treatment
- pathogen infectione0
- Interactor
- Fusarium oxysporumFusarium oxysporumt0pathogen
- Outcome
- MeJAe0
- Direction
- earlier and highere0
- Comparator
- ‘Solex’e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pathogen infection causes a differential accumulation of SA, MeSA, and MeJA in a resistant cultivar (i.e. ‘Golem’), being earlier and higher than that observed in a susceptible one (i.e. ‘Solex’)”
t0“carnation-Fusarium oxysporum”
Claim · model-written summary
Guaiacol peroxidase activity increased during the resistance response.
- Taxon
- carnationDianthus caryophyllust0
- Gene
- guaiacol peroxidasee0
- Outcome
- guaiacol peroxidase enzymatic activitye0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase of guaiacol peroxidase enzymatic activity (GPX)”
t0“carnation”
Claim · model-written summary
Transcription of a symplastic peroxidase gene increased during the resistance response.
- Taxon
- carnationDianthus caryophyllust0
- Gene
- a gene coding for a symplastic peroxidasee0
- Outcome
- transcriptional levelse0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase of guaiacol peroxidase enzymatic activity (GPX) and transcriptional levels of a gene coding for a symplastic peroxidase”
t0“carnation”
Claim · model-written summary
Salicylic acid accumulation was positively correlated with GPX activity and peroxidase mRNA levels.
- Taxon
- carnationDianthus caryophyllust0
- Gene
- peroxidasese0
- Compound
- SAe0
- Outcome
- GPX activity and mRNA levelse0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The positive statistical correlation between the accumulation of SA and MeJA and the expression of peroxidases (GPX activity and mRNA levels)”
t0“carnation”
Claim · model-written summary
Methyl jasmonate accumulation was positively correlated with GPX activity and peroxidase mRNA levels.
- Taxon
- carnationDianthus caryophyllust0
- Gene
- peroxidasese0
- Compound
- MeJAe0
- Outcome
- GPX activity and mRNA levelse0
- Direction
- positivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The positive statistical correlation between the accumulation of SA and MeJA and the expression of peroxidases (GPX activity and mRNA levels)”
t0“carnation”