Abstract
Phosphorus (P) is a nonrenewable resource, which is one of the major challenges for sustainable agriculture. Although phosphite (Phi) can be absorbed by the plant cells through the Pi transporters, it cannot be metabolized by plant and unable to use as P fertilizers for crops. However, transgenic plants that overexpressed phosphite dehydrogenase (PtxD) from bacteria can utilize phosphite as the sole P source. In this study, we aimed to improve the catalytic efficiency of PtxD from Ralstonia sp.4506 (PtxD R4506 ), by directed evolution. Five mutations were generated by saturation mutagenesis at the 139th site of PtxD R4506 and showed higher catalytic efficiency than native PtxD R4506 . The PtxD Q showed the highest catalytic efficiency (5.83-fold as compared to PtxD R4506 ) contributed by the 41.1% decrease in the K m and 2.5-fold increase in the k cat values. Overexpression of PtxD Q in Arabidopsis and rice showed increased efficiency of phosphite utilization and excellent development when phosphite was used as the primary source of P. High-efficiency PtxD transgenic plant is an essential prerequisite for future agricultural production using phosphite as P fertilizers.
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Claims 8
Claim · model-written summary
Five PtxD R4506 mutations had higher catalytic efficiency than the native enzyme.
Verbatim
e0“Five mutations were generated by saturation mutagenesis at the 139th site of PtxD R4506 and showed higher catalytic efficiency than native PtxD R4506”
Claim · model-written summary
PtxD Q had 5.83-fold catalytic efficiency relative to PtxD R4506.
Not reportedTaxon
Verbatim
e0“The PtxD Q showed the highest catalytic efficiency (5.83-fold as compared to PtxD R4506 )”
Claim · model-written summary
PtxD Q had a 41.1% decrease in Km.
Not reportedTaxon
Verbatim
e0“41.1% decrease in the K m”
Claim · model-written summary
PtxD Q had a 2.5-fold increase in kcat.
Not reportedTaxon
Verbatim
e0“2.5-fold increase in the k cat values”
Claim · model-written summary
PtxD Q overexpression increased phosphite utilization in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Overexpression of PtxD Qe0
- Gene
- PtxD Qe0
- Outcome
- efficiency of phosphite utilizatione0
- Direction
- increasede0
- Qualifier
- phosphite was used as the primary source of Pe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PtxD Q in Arabidopsis and rice showed increased efficiency of phosphite utilization and excellent development when phosphite was used as the primary source of P”
Claim · model-written summary
PtxD Q overexpression increased phosphite utilization in rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- Overexpression of PtxD Qe0
- Gene
- PtxD Qe0
- Outcome
- efficiency of phosphite utilizatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PtxD Q in Arabidopsis and rice showed increased efficiency of phosphite utilization and excellent development when phosphite was used as the primary source of P”
Claim · model-written summary
Arabidopsis with PtxD Q showed excellent development using phosphite as the main phosphorus source.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- PtxD Qe0
- Outcome
- excellent developmente0
- Qualifier
- phosphite was used as the primary source of Pe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PtxD Q in Arabidopsis and rice showed increased efficiency of phosphite utilization and excellent development when phosphite was used as the primary source of P”
Claim · model-written summary
Rice with PtxD Q showed excellent development using phosphite as the main phosphorus source.
- Taxon
- riceOryza sativae0
- Gene
- PtxD Qe0
- Outcome
- excellent developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PtxD Q in Arabidopsis and rice showed increased efficiency of phosphite utilization and excellent development when phosphite was used as the primary source of P”