Abstract
Somatic embryogenesis was used as a tool for micropropagation of wild Narcissus tazetta plants exposing to overcollection and rapidly changed environmental conditions. In the growth regulators experiment, somatic embryogenesis was successfully induced in all treatments except for explants grown in the control treatment (Murashig and Skoog (MS) hormone-free medium). Meanwhile, the highest value for the number of somatic embryos/ callus segment (441) was obtained into MS media supplemented with 0.2 mg l -1 6-(gamma, gamma-Dimethylallylamino) purine (2iP) under dark conditions. Moreover, sucrose at 30g L -1 was the best sugar source resulting in higher number of somatic embryos compared to the other sugar treatments. The highest shoot development rate from somatic embryos was (191.99/ non-embryonic calli segment) recorded in cultures grown on MS media plus 0.5 mg l -1 2iP. Maximum bulbet size (1.6 cm diameter) was recorded in plantlets kept onto hormone-free MS hormone free media for 6 weeks before acclimatization, while less durations resulted in smaller bulbet size. Well developed in vitro plantlets were acclimatized successfully with high survival percentage of (95%). The acclimatized plants were normal and did not show any morphological abnormalities.
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Claims 7
Claim · model-written summary
Embryogenesis occurred under all growth-regulator treatments except the hormone-free control.
- Taxon
- Narcissus tazetta
Narcissus tazettat0 - Treatment
- all treatmentse0
- Outcome
- somatic embryogenesise0
- Comparator
- control treatmente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“somatic embryogenesis was successfully induced in all treatments except for explants grown in the control treatment (Murashig and Skoog (MS) hormone-free medium)”
t0“Narcissus tazetta”
Claim · model-written summary
The 0.2 mg l -1 2iP dark treatment produced 441 somatic embryos per callus segment.
- Taxon
- Narcissus tazettaNarcissus tazettat0
- Treatment
- 0.2 mg l -1 6-(gamma, gamma-Dimethylallylamino) purine (2iP)e0
- Outcome
- number of somatic embryos/ callus segmente0
- Direction
- higheste0
- Value
- 441e0
- Setting
- dark conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest value for the number of somatic embryos/ callus segment (441) was obtained into MS media supplemented with 0.2 mg l -1 6-(gamma, gamma-Dimethylallylamino) purine (2iP) under dark conditions”
t0“Narcissus tazetta”
Claim · model-written summary
Sucrose at 30g L -1 produced more somatic embryos than other sugars.
- Taxon
- Narcissus tazettaNarcissus tazettat0
- Treatment
- sucrose at 30g L -1e0
- Outcome
- number of somatic embryose0
- Direction
- highere0
- Comparator
- other sugar treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose at 30g L -1 was the best sugar source resulting in higher number of somatic embryos compared to the other sugar treatments”
t0“Narcissus tazetta”
Claim · model-written summary
MS medium with 0.5 mg l -1 2iP gave the highest shoot development rate, 191.99 per non-embryonic calli segment.
- Taxon
- Narcissus tazettaNarcissus tazettat0
- Treatment
- MS media plus 0.5 mg l -1 2iPe0
- Outcome
- shoot development rate from somatic embryose0
- Direction
- higheste0
- Value
- 191.99/ non-embryonic calli segmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest shoot development rate from somatic embryos was (191.99/ non-embryonic calli segment) recorded in cultures grown on MS media plus 0.5 mg l -1 2iP”
t0“Narcissus tazetta”
Claim · model-written summary
Six weeks on hormone-free medium yielded the maximum bulbet diameter of 1.6 cm.
- Taxon
- Narcissus tazettaNarcissus tazettat0
- Treatment
- hormone-free MS hormone free media for 6 weekse0
- Outcome
- bulbet sizee0
- Direction
- Maximume0
- Value
- 1.6 cm diametere0
- Comparator
- less durationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum bulbet size (1.6 cm diameter) was recorded in plantlets kept onto hormone-free MS hormone free media for 6 weeks before acclimatization, while less durations resulted in smaller bulbet size”
t0“Narcissus tazetta”
Claim · model-written summary
Acclimatized plantlets survived at 95 per cent.
- Taxon
- Narcissus tazettaNarcissus tazettat0
- Outcome
- survival percentagee0
- Direction
- highe0
- Value
- 95%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Well developed in vitro plantlets were acclimatized successfully with high survival percentage of (95%)”
t0“Narcissus tazetta”
Claim · model-written summary
Acclimatized plants showed normal morphology without abnormalities.
- Taxon
- Narcissus tazettaNarcissus tazettat0
- Outcome
- normal and did not show any morphological abnormalitiese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The acclimatized plants were normal and did not show any morphological abnormalities”
t0“Narcissus tazetta”
The same questions in other papers
sucrose · growth · Narcissus tazettaResults agree
1 paper in the same direction as this paper
- positive
radiation · growth · Narcissus tazettaPositive and negative results
1 in the opposite direction
- no effect
class · growth · Narcissus tazettaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.