Abstract
Topping and salicylic acid (SA) treatment are thought to change nicotine content in Nicotiana tabacum L. (tobacco). This study aimed to investigate the potential underlying molecular mechanisms. Tobacco variety K326 plants were divided into four groups, each receiving only the topping treatment (TW), the topping and 0.6 mM SA treatment (TS), the 0.6 mM SA treatment only (NS), and no treatment (control group, NW). The content of alkaloids and expression of genes related to nicotine synthesis were determined through gas chromatography-hydrogen flame ionization and liquid chromatography mass spectrometry. Differentially expressed and differentially expressed genes (DEGs) were screened and analyzed between different groups. Finally, we constructed a DEG-pathway-differential metabolite network using Cytoscape. While SA treatment decreased the expression of genes related to nicotine synthesis and alkaloid content, topping showed the opposite results: transcriptome and metabolome analyses showed that both changed a variety of biological pathways and related gene expression in tobacco, including arginine and proline metabolism, tropane, piperidine and pyridine alkaloid biosynthesis, nicotinate and nicotinamide metabolism, glutathione metabolism, biotin metabolism, phenylpropanoid biosynthesis, and plant hormone signal transduction. This study provides new insights for understanding how topping and SA treatment affect nicotine content in tobacco and provides a direction for future breeding.
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Claims 5
Claim · model-written summary
Salicylic acid decreased expression of nicotine synthesis genes.
- Taxon
- Nicotiana tabacum L.
Nicotiana tabacumt0 - Treatment
- SA treatmente0
- Outcome
- expression of genes related to nicotine synthesise0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA treatment decreased the expression of genes related to nicotine synthesis and alkaloid content”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Salicylic acid decreased alkaloid content.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Treatment
- SA treatmente0
- Outcome
- alkaloid contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA treatment decreased the expression of genes related to nicotine synthesis and alkaloid content”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Topping increased expression of genes related to nicotine synthesis.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Treatment
- toppinge0
- Outcome
- expression of genes related to nicotine synthesise0
- Direction
- increaseinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“While SA treatment decreased the expression of genes related to nicotine synthesis and alkaloid content, topping showed the opposite results”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Topping increased alkaloid content.
- Taxon
- Nicotiana tabacum L.Nicotiana tabacumt0
- Treatment
- toppinge0
- Outcome
- alkaloid contente0
- Direction
- increaseinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“While SA treatment decreased the expression of genes related to nicotine synthesis and alkaloid content, topping showed the opposite results”
t0“Nicotiana tabacum L.”
Claim · model-written summary
Both treatments changed multiple metabolic and signaling pathways and associated tobacco gene expression.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- bothe0
- Outcome
- biological pathways and related gene expressione0
- Direction
- changede0
- Qualifier
- arginine and proline metabolism, tropane, piperidine and pyridine alkaloid biosynthesis, nicotinate and nicotinamide metabolism, glutathione metabolism, biotin metabolism, phenylpropanoid biosynthesis, and plant hormone signal transductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptome and metabolome analyses showed that both changed a variety of biological pathways and related gene expression in tobacco, including arginine and proline metabolism, tropane, piperidine and pyridine alkaloid biosynthesis, nicotinate and nicotinamide metabolism, glutathione metabolism, biotin metabolism, phenylpropanoid biosynthesis, and plant hormone signal transduction”
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