Abstract
Increasing drought tolerance of sugarcane can be done through mutation induction using gamma ray irradiation and continued by in vitro selection. This research aimed to evaluate the response of gamma-irradiated calli and drought tolerance of putative mutants through in vitro selection using selection agent polyethylene glycol (PEG). PSJT 941 variety was used as mother plant in this study. Embryogenic callus was induced on MS media supplemented with 3 mg/l 2.4-D. Six levels of gamma ray irradiation were used (0, 10, 20, 30, 40, and 50 Gy), while two levels of PEG were used for in vitro selection (0 and 10%). The observed variables were percentage of surviving calli, percentage of regenerated calli, number of shoots, shoot height, and relative decrease index. The results showed that the ability of callus to regenerate decreased with increasing doses of gamma ray irradiation. Relative decrease index of percentage of surviving calli and number of shoots emerged in irradiated calli with gamma ray doses of 10, 30, and 40 Gy in 10% PEG selection media were lower than the control (0 Gy). It was concluded that gamma ray irradiation doses of 10, 30, and 40 Gy and 10% PEG suitable to produce drought tolerance of sugarcane.
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Claims 3
Claim · model-written summary
Callus regeneration ability in sugarcane decreased with increasing gamma ray irradiation doses.
- Taxon
- sugarcanet0
- Material
- PSJT 941e1
- Treatment
- increasing doses of gamma ray irradiatione2
- Outcome
- ability of callus to regeneratee2
- Direction
- decreasede2
Not reportedComparator
Verbatim
t0“drought tolerance of sugarcane”
e1“PSJT 941 variety was used as mother plant in this study”
e2“the ability of callus to regenerate decreased with increasing doses of gamma ray irradiation”
Claim · model-written summary
Relative decrease index of surviving calli and shoot number in irradiated sugarcane calli at 10, 30 and 40 Gy under 10% PEG was lower than the 0 Gy control.
Verbatim
t0“drought tolerance of sugarcane”
e3“Relative decrease index of percentage of surviving calli and number of shoots emerged in irradiated calli with gamma ray doses of 10, 30, and 40 Gy in 10% PEG selection media were lower than the control (0 Gy)”
Claim · model-written summary
Gamma ray doses of 10, 30 and 40 Gy with 10% PEG are suitable for producing drought tolerance in sugarcane.
Verbatim
t0“drought tolerance of sugarcane”
e4“It was concluded that gamma ray irradiation doses of 10, 30, and 40 Gy and 10% PEG suitable to produce drought tolerance of sugarcane”
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