Paper · 2022 · PLoS ONE

Seed nano-priming with Zinc Oxide nanoparticles in rice mitigates drought and enhances agronomic profile

Muhammad Waqas Mazhar; Muhammad Ishtiaq; Iqbal Hussain; et al.

17
claims
34
verbatim quotes
150
citations
2
research areas
✓
Open access · cc-by

Abstract

All cereal crops, particularly rice are perpetually affected due to drastic climatic changes which triggers different stressors resulting in food shortage scenarios across the globe. In modern era, application of nanotechnology holds the pledge in combating the climate change mediated environmental stressors through nanomaterials such as pesticides, nano-biosensors, nano-clays and nano-seed priming technologies. Current study is a part of experiment conducted to comprehend the behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment. The seed priming treatment concentrations included 0, 5, 10, 15, 25 and 50 ppm. In the experimental results an increase in plant height, total chlorophyll contents, plant fresh and dry weights was obtained by use of seed priming with ZnONPs. The study results proved that seed priming with 25ppm of ZnONPs increased seed and straw yield with value of 85.333 and 123.333, respectively under water deficit environment. The analysis depicted that 25 ppm has been found more suitable for increasing the 1000 paddy weight of rice plants under both well irrigated and water shortage conditions. Seed priming with ZnONPs results in 53% reduction in MDA contents of water stressed rice plants Drought stress leads to reduction in plant height by 31%, plant fresh weight by 22% and plant dry weight by 28%. Seed priming treatments imparted in current study show significance increase in plant biomass. Priming with ZnONPs further enhances the levels of proline amino acid facilitating the plant to combat water shortage stress. A further elevation in activities of SOD, CAT and POD takes place in rice plants raised from ZnONPs primed seeds by 11%, 13% and 38%, respectively. An elevation in activities of antioxidant enzymes was found and the levels of oxidative stress indicators decreased upon seed priming with ZnONPs. Furthermore the yield characteristics such as panicle length, number of tillers, paddy yield and straw yield of the rice plants raised through ZnONPs primed seeds enhanced. The ZnONPs at concentration of 25 ppm proved optimum in alleviating drought induced damages. It can be inferred that seed pre conditioning with ZnONPs is helpful in increasing yield attributes under the water shortage environment.

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Claims 17

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed priming with ZnO nanoparticles increased plant height and total chlorophyll content in rice.

Treatment
seed priming with ZnONPse1
Outcome
plant height, total chlorophyll contentse1
Direction
increasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e1

“an increase in plant height, total chlorophyll contents, plant fresh and dry weights was obtained by use of seed priming with ZnONPs”

✓ located verbatim in the source · abstract, characters 712–844 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed priming with ZnO nanoparticles increased plant fresh and dry weights in rice.

Treatment
seed priming with ZnONPse1
Outcome
plant fresh and dry weightse1
Direction
increasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e1

“an increase in plant height, total chlorophyll contents, plant fresh and dry weights was obtained by use of seed priming with ZnONPs”

✓ located verbatim in the source · abstract, characters 712–844 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed priming with 25 ppm ZnO nanoparticles increased seed yield in rice under water deficit (value 85.333).

Treatment
seed priming with 25ppm of ZnONPse2
Outcome
seed and straw yielde2
Direction
increasede2
Value
85.333e2
Qualifier
respectivelye2
Setting
water deficit environmente2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e2

“seed priming with 25ppm of ZnONPs increased seed and straw yield with value of 85.333 and 123.333, respectively under water deficit environment”

✓ located verbatim in the source · abstract, characters 876–1019 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed priming with 25 ppm ZnO nanoparticles increased straw yield in rice under water deficit (value 123.333).

Treatment
seed priming with 25ppm of ZnONPse2
Outcome
seed and straw yielde2
Direction
increasede2
Value
123.333e2
Qualifier
respectivelye2
Setting
water deficit environmente2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e2

“seed priming with 25ppm of ZnONPs increased seed and straw yield with value of 85.333 and 123.333, respectively under water deficit environment”

✓ located verbatim in the source · abstract, characters 876–1019 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

25 ppm ZnO nanoparticle seed priming is more suitable for increasing the 1000 paddy weight of rice under both well irrigated and water shortage conditions.

Treatment
25 ppme3
Outcome
1000 paddy weighte3
Direction
increasinge3
Setting
well irrigated and water shortage conditionse3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e3

“25 ppm has been found more suitable for increasing the 1000 paddy weight of rice plants under both well irrigated and water shortage conditions”

✓ located verbatim in the source · abstract, characters 1048–1191 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed priming with ZnO nanoparticles reduced MDA content by 53% in water-stressed rice.

Treatment
Seed priming with ZnONPse4
Outcome
MDA contentse4
Direction
reductione4
Value
53%e4
Qualifier
water stressede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e4

“Seed priming with ZnONPs results in 53% reduction in MDA contents of water stressed rice plants”

✓ located verbatim in the source · abstract, characters 1193–1288 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Drought stress reduced rice plant height by 31%.

Treatment
Drought stresse5
Outcome
plant heighte5
Direction
reductione5
Value
31%e5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e5

“Drought stress leads to reduction in plant height by 31%, plant fresh weight by 22% and plant dry weight by 28%”

✓ located verbatim in the source · abstract, characters 1289–1400 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Drought stress reduced rice plant fresh weight by 22%.

Treatment
Drought stresse5
Outcome
plant fresh weighte5
Direction
reductione5
Value
22%e5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e5

“Drought stress leads to reduction in plant height by 31%, plant fresh weight by 22% and plant dry weight by 28%”

✓ located verbatim in the source · abstract, characters 1289–1400 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Drought stress reduced rice plant dry weight by 28%.

Treatment
Drought stresse5
Outcome
plant dry weighte5
Direction
reductione5
Value
28%e5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e5

“Drought stress leads to reduction in plant height by 31%, plant fresh weight by 22% and plant dry weight by 28%”

✓ located verbatim in the source · abstract, characters 1289–1400 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

Priming with ZnO nanoparticles enhanced proline levels in rice facing water shortage.

Treatment
Priming with ZnONPse6
Outcome
levels of proline amino acide6
Direction
enhancese6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e6

“Priming with ZnONPs further enhances the levels of proline amino acid facilitating the plant to combat water shortage stress”

✓ located verbatim in the source · abstract, characters 1497–1621 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Rice raised from ZnO-primed seeds shows a 11% elevation in SOD activity.

Treatment
ZnONPs primed seedse7
Gene
SODe7
Outcome
activities of SOD, CAT and PODe7
Direction
elevatione7
Value
11%e7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e7

“A further elevation in activities of SOD, CAT and POD takes place in rice plants raised from ZnONPs primed seeds by 11%, 13% and 38%, respectively”

✓ located verbatim in the source · abstract, characters 1623–1769 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Rice raised from ZnO-primed seeds shows a 13% elevation in CAT activity.

Treatment
ZnONPs primed seedse7
Gene
CATe7
Outcome
activities of SOD, CAT and PODe7
Direction
elevatione7
Value
13%e7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e7

“A further elevation in activities of SOD, CAT and POD takes place in rice plants raised from ZnONPs primed seeds by 11%, 13% and 38%, respectively”

✓ located verbatim in the source · abstract, characters 1623–1769 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Rice raised from ZnO-primed seeds shows a 38% elevation in POD activity.

Treatment
ZnONPs primed seedse7
Gene
PODe7
Outcome
activities of SOD, CAT and PODe7
Direction
elevatione7
Value
38%e7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e7

“A further elevation in activities of SOD, CAT and POD takes place in rice plants raised from ZnONPs primed seeds by 11%, 13% and 38%, respectively”

✓ located verbatim in the source · abstract, characters 1623–1769 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed priming with ZnO nanoparticles elevated antioxidant enzyme activities in rice.

Treatment
seed priming with ZnONPse1
Outcome
activities of antioxidant enzymese1
Direction
elevatione1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e1

“An elevation in activities of antioxidant enzymes was found and the levels of oxidative stress indicators decreased upon seed priming with ZnONPs”

✓ located verbatim in the source · abstract, characters 1771–1916 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed priming with ZnO nanoparticles decreased oxidative stress indicators in rice.

Treatment
seed priming with ZnONPse1
Outcome
levels of oxidative stress indicatorse1
Direction
decreasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e1

“An elevation in activities of antioxidant enzymes was found and the levels of oxidative stress indicators decreased upon seed priming with ZnONPs”

✓ located verbatim in the source · abstract, characters 1771–1916 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed priming with ZnO nanoparticles enhanced panicle length, tiller number and paddy yield in rice.

Treatment
ZnONPs primed seedse2
Outcome
panicle length, number of tillers, paddy yielde2
Direction
enhancede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e2

“the yield characteristics such as panicle length, number of tillers, paddy yield and straw yield of the rice plants raised through ZnONPs primed seeds enhanced”

✓ located verbatim in the source · abstract, characters 1930–2089 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

ZnO nanoparticles at 25 ppm were optimal for alleviating drought-induced damage in rice.

Treatment
ZnONPs at concentration of 25 ppme3
Outcome
alleviating drought induced damagese3
Direction
optimume3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“behaviour of rice plants raised from Zinc Oxide nanoparticles (ZnONPs) primed seeds under the water shortage environment”

✓ located verbatim in the source · abstract, characters 482–602 · show in text ↑
e3

“The ZnONPs at concentration of 25 ppm proved optimum in alleviating drought induced damages”

✓ located verbatim in the source · abstract, characters 2091–2182 · show in text ↑

zinc oxide nanoparticle · physiology: pigments · Oryza sativa (rice)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

zinc oxide nanoparticle · physiology: oxidative · Oryza sativa (rice)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

zinc oxide nanoparticle · physiology: antioxidant enzymes · Oryza sativa (rice)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

zinc oxide nanoparticle · physiology: osmolytes · Oryza sativa (rice)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

zinc oxide nanoparticle · yield · Oryza sativa (rice)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

zinc oxide nanoparticle · growth · Oryza sativa (rice)Results agree

7 papers: 7 positive, 0 negative or no effect

6 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

3 papers more in this group

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seed treatment · physiology: pigments · Oryza sativa (rice)Positive and negative results

18 papers: 17 positive, 1 negative or no effect

16 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

14 papers more in this group

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seed treatment · physiology: antioxidant enzymes · Oryza sativa (rice)Positive and negative results

31 papers: 30 positive, 1 negative or no effect

29 papers in the same direction as this paper · 1 reporting no difference from an alternative

  1. no effect
  2. positive
  3. positive

27 papers more in this group

See all 31 papers →

8 of 13 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.