Abstract
Tomato is one of the major economically domesticated crops, and it is extensively used in different ways and purposes worldwide. Cell metabolism is the central core of all the biological processes to sustain life including cell growth, differentiation, maintenance, and response to environmental stress. To evaluate how genetic engineering can improve tomato fruit metabolome, the transcriptomic and metabolomic datasets of two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type. The combined results demonstrated that the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids as has been stated in several studies, but has also up- and down-regulated the expression of multiple genes related to cell growth (cell wall turnover), primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolism, cell signaling, and stress responses. These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality, biosynthesis of health-promoting phytochemicals (including phenolic acids, flavonoids, and anthocyanins), and finally, activate resistance to Botrytis cinerea and repress the expression of over-ripening-related genes, thus extending the fruit shelf-life. In conclusion, the traits improvement achieved by SlMX1 overexpression can be harnessed in molecular breeding programs to engineer fruit size and yield, induce health-promoting secondary metabolites, promote fungal resistance, and finally extend the fruit shelf-life.
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Claims 10
Claim · model-written summary
Constitutive SlMX1 expression increased trichome formation, carotenoids and terpenoids in tomato relative to wild-type.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- constitutive expression of SlMX1e2
- Outcome
- trichome formation, carotenoids, and terpenoidse2
- Direction
- increasede2
- Comparator
- wild-typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e1“two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type”
e2“the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids”
Claim · model-written summary
Constitutive SlMX1 expression up- and down-regulated multiple genes related to cell growth (cell wall turnover) in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- constitutive expression of SlMX1e2
- Outcome
- expression of multiple genes related to cell growth (cell wall turnover)e3
- Direction
- up- and down-regulatede3
- Comparator
- wild-typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e1“two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type”
e2“the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids”
e3“has also up- and down-regulated the expression of multiple genes related to cell growth (cell wall turnover), primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolism, cell signaling, and stress responses”
Claim · model-written summary
Constitutive SlMX1 expression up- and down-regulated genes of primary and secondary metabolism in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- constitutive expression of SlMX1e2
- Outcome
- primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolisme3
- Direction
- up- and down-regulatede3
- Comparator
- wild-typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e1“two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type”
e2“the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids”
e3“has also up- and down-regulated the expression of multiple genes related to cell growth (cell wall turnover), primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolism, cell signaling, and stress responses”
Claim · model-written summary
Constitutive SlMX1 expression up- and down-regulated genes for cell signalling and stress responses in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- constitutive expression of SlMX1e2
- Outcome
- cell signaling, and stress responsese3
- Direction
- up- and down-regulatede3
- Comparator
- wild-typee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e1“two transgenic tomatoes (SlMX1 overexpression and RNAi lines) have been compared with wild-type”
e2“the constitutive expression of SlMX1 not only increased trichome formation, carotenoids, and terpenoids”
e3“has also up- and down-regulated the expression of multiple genes related to cell growth (cell wall turnover), primary (carbohydrates, vitamins, and phytohormones), and secondary (phenylpropanoids, carotenoids, and terpenoids) metabolism, cell signaling, and stress responses”
Claim · model-written summary
Constitutive SlMX1 expression promotes fruit yield and quality in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- constitutive expression of SlMX1e4
- Outcome
- fruit yield and qualitye4
- Direction
- promotee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e4“These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality”
Claim · model-written summary
Constitutive SlMX1 expression promotes biosynthesis of health-promoting phytochemicals in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- constitutive expression of SlMX1e4
- Outcome
- biosynthesis of health-promoting phytochemicals (including phenolic acids, flavonoids, and anthocyanins)e5
- Direction
- promotee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e4“These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality”
e5“biosynthesis of health-promoting phytochemicals (including phenolic acids, flavonoids, and anthocyanins)”
Claim · model-written summary
Constitutive SlMX1 expression activates resistance to Botrytis cinerea in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- constitutive expression of SlMX1e4
- Interactor
- Botrytis cinerea
Botrytis cinereae6pathogen - Outcome
- resistancee6
- Direction
- activatee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e4“These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality”
e6“activate resistance to Botrytis cinerea”
Claim · model-written summary
Constitutive SlMX1 expression represses over-ripening-related gene expression in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- constitutive expression of SlMX1e4
- Outcome
- expression of over-ripening-related genese7
- Direction
- represse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e4“These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality”
e7“repress the expression of over-ripening-related genes, thus extending the fruit shelf-life”
Claim · model-written summary
Constitutive SlMX1 expression extends tomato fruit shelf-life.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- constitutive expression of SlMX1e4
- Outcome
- fruit shelf-lifee7
- Direction
- extendinge7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e4“These changes in gene expression due to the constitutive expression of SlMX1 promote the most important agroeconomic traits such as fruit yield and quality”
e7“repress the expression of over-ripening-related genes, thus extending the fruit shelf-life”
Claim · model-written summary
SlMX1 traits can be harnessed in molecular breeding programmes to engineer tomato fruit size and yield.
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- engineer fruit size and yielde1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato is one of the major economically domesticated crops”
e1“can be harnessed in molecular breeding programs to engineer fruit size and yield”
The same questions in other papers
overexpression · quality: phytochemicals · Solanum lycopersicum (tomato)Positive and negative results
2 in the opposite direction
- negative
- negative
overexpression · quality: postharvest · Solanum lycopersicum (tomato)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
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See all 6 papers →overexpression · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- negative
6 papers more in this group
See all 10 papers →overexpression · Botrytis cinerea · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.