Abstract
Common in Fungal Extracellular Membrane (CFEM) domain proteins are considered to be unique to fungi and closely related to pathogenicity. However, the Puccinia striiformis f. sp. tritici (Pst) effector containing the CFEM domain has not been reported. Here, we obtained an effector, PstCFEM1, containing a functional N-terminal signal peptide sequence and the CFEM domain from Pst race CYR31. qRT-PCR assay indicated that the transcript levels of PstCFEM1 were highly induced during the early stages of infection. Overexpression of PstCFEM1 suppressed Pst322 (an elicitor-like protein of Pst)-trigged cell death, reactive oxygen species (ROS) accumulation and callose deposition. Host-induced gene silencing (HIGS) experiments showed that knockdown of PstCFEM1 decreased the virulence of Pst, while ROS accumulation in silenced plants increased near the infection site. In addition, wheat containing the PstCFEM1-silenced construct increased resistance to multiple races of Pst. Our data suggest that PstCFEM1 suppresses wheat defense by inhibiting ROS accumulation and contributes to increased virulence of Pst.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
PstCFEM1 has a functional N-terminal signal peptide and CFEM domain.
- Taxon
- wheat
Triticum aestivumt0 - Interactor
- Pst race CYR31e0pathogen
- Gene
- PstCFEM1e0
- Outcome
- containing a functional N-terminal signal peptide sequence and the CFEM domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an effector, PstCFEM1, containing a functional N-terminal signal peptide sequence and the CFEM domain from Pst race CYR31”
t0“wheat”
Claim · model-written summary
PstCFEM1 transcripts were highly induced early in infection.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie1pathogen - Gene
- PstCFEM1e0
- Outcome
- transcript levels of PstCFEM1e0
- Direction
- highly inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript levels of PstCFEM1 were highly induced during the early stages of infection”
t0“wheat”
e1“Puccinia striiformis f. sp. tritici (Pst)”
Claim · model-written summary
PstCFEM1 overexpression suppressed Pst322-triggered cell death.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Overexpression of PstCFEM1e0
- Interactor
- Pste0pathogen
- Gene
- PstCFEM1e0
- Outcome
- Pst322 (an elicitor-like protein of Pst)-trigged cell deathe0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PstCFEM1 suppressed Pst322 (an elicitor-like protein of Pst)-trigged cell death, reactive oxygen species (ROS) accumulation and callose deposition”
t0“wheat”
Claim · model-written summary
PstCFEM1 overexpression suppressed ROS accumulation and callose deposition.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Overexpression of PstCFEM1e0
- Interactor
- Pste0pathogen
- Gene
- PstCFEM1e0
- Outcome
- reactive oxygen species (ROS) accumulation and callose depositione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PstCFEM1 suppressed Pst322 (an elicitor-like protein of Pst)-trigged cell death, reactive oxygen species (ROS) accumulation and callose deposition”
t0“wheat”
Claim · model-written summary
Silencing PstCFEM1 decreased Pst virulence.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- knockdown of PstCFEM1e0
- Interactor
- Pste0pathogen
- Gene
- PstCFEM1e0
- Outcome
- virulence of Pste0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockdown of PstCFEM1 decreased the virulence of Pst”
t0“wheat”
Claim · model-written summary
ROS accumulation increased near infection sites in silenced wheat plants.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- knockdown of PstCFEM1e1
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie2pathogen
- Gene
- PstCFEM1e1
- Outcome
- ROS accumulatione0
- Direction
- increasede0
- Setting
- near the infection sitee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS accumulation in silenced plants increased near the infection site”
e1“knockdown of PstCFEM1”
t0“wheat”
e2“Puccinia striiformis f. sp. tritici (Pst)”
Claim · model-written summary
Wheat containing the PstCFEM1-silenced construct showed increased resistance to multiple Pst races.
- Taxon
- wheatTriticum aestivume0
- Treatment
- PstCFEM1-silenced constructe0
- Interactor
- Pste0pathogen
- Gene
- PstCFEM1e0
- Outcome
- resistance to multiple races of Pste0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wheat containing the PstCFEM1-silenced construct increased resistance to multiple races of Pst”
Claim · model-written summary
The authors suggest that PstCFEM1 suppresses wheat defense by inhibiting ROS accumulation.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Puccinia striiformis f. sp. triticiPuccinia striiformis f. sp. triticie1pathogen
- Gene
- PstCFEM1e0
- Outcome
- wheat defensee0
- Qualifier
- inhibiting ROS accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PstCFEM1 suppresses wheat defense by inhibiting ROS accumulation”
e1“Puccinia striiformis f. sp. tritici (Pst)”
Claim · model-written summary
The authors suggest that PstCFEM1 increases Pst virulence.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Pste0pathogen
- Gene
- PstCFEM1e1
- Outcome
- virulence of Pste0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contributes to increased virulence of Pst”
e1“PstCFEM1 suppresses wheat defense”
t0“wheat”
The same questions in other papers
loss of function · disease · Triticum aestivum (wheat)Positive and negative results
80 papers in the same direction as this paper · 96 in the opposite direction
- negative
- negative
- positive
173 papers more in this group
See all 177 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.