Abstract
Juice sac granulation (a physiological disorder) leads to large postharvest losses of pomelo (Citrus maxima). Previous studies have shown that juice sac granulation is closely related to lignin accumulation, while the molecular mechanisms underlying this disorder remain elusive in pomelo. Our results showed that the lignin content in NC (near the core) and FC (far away from the core) juice sacs overall increased from 157 DPA (days post anthesis) to 212 DPA and reached a maximum at 212 DPA. Additionally, the lignin content of NC juice sacs was higher than that of FC juice sacs. In this study, we used transcriptome-based weighted gene co-expression network analysis (WGCNA) to address how lignin formation in NC and FC juice sacs is generated during the development of pomelo. After data assembly and bioinformatic analysis, we found a most correlated module (black module) to the lignin content, then we used the 11 DEGs in this module as hub genes for lignin biosynthesis. Among these DEGs, PAL (phenylalanine ammonia lyase), HCT (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase), 4CL2 (4-coumarate: CoA ligase), C4H (cinnamate 4-hydroxylase), C3′H (p-coumarate 3-hydroxylase), and CCoAOMT1 (caffeoyl CoA 3-Omethyltransferase) were the most distinct DEGs in granulated juice sacs. Co-expression analysis revealed that the expression patterns of several transcription factors such as MYB, NAC, OFP6, and bHLH130 are highly correlated with lignin formation. In addition, the expression patterns of the DEGs related to lignin biosynthesis and transcription factors were validated by qRT-PCR, and the results were highly concordant with the RNA-seq results. These results would be beneficial for further studies on the molecular mechanism of lignin accumulation in pomelo juice sacs and would help with citrus breeding.
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Claims 9
Claim · model-written summary
Lignin content in both juice sac positions increased from 157 to 212 DPA and peaked at 212 DPA.
- Taxon
- pomelo
Citrus maximat0 - Organ / stage
- juice sacse0
- Outcome
- lignin contente0
- Direction
- increasede0
- Value
- 212 DPAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lignin content in NC (near the core) and FC (far away from the core) juice sacs overall increased from 157 DPA (days post anthesis) to 212 DPA and reached a maximum at 212 DPA”
t0“Pomelo (Citrus maxima)”
Claim · model-written summary
Near-core juice sacs had higher lignin content than far-core juice sacs.
- Taxon
- pomeloCitrus maximat0
- Organ / stage
- NC juice sacse0
- Outcome
- lignin contente0
- Direction
- highere0
- Comparator
- FC juice sacse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lignin content of NC juice sacs was higher than that of FC juice sacs”
t0“Pomelo (Citrus maxima)”
Claim · model-written summary
The black co-expression module was most correlated with lignin content.
- Taxon
- pomeloCitrus maximat0
- Material
- black modulee0
- Outcome
- lignin contente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we found a most correlated module (black module) to the lignin content”
t0“Pomelo (Citrus maxima)”
Claim · model-written summary
Eleven DEGs in the module were identified as hub genes for lignin biosynthesis.
- Taxon
- pomeloCitrus maximat0
- Gene
- DEGse0
- Outcome
- hub genes for lignin biosynthesise0
- Value
- 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 11 DEGs in this module as hub genes for lignin biosynthesis”
t0“Pomelo (Citrus maxima)”
Claim · model-written summary
PAL, HCT and 4CL2 were among the most distinct DEGs in granulated juice sacs.
- Taxon
- pomeloCitrus maximat0
- Organ / stage
- granulated juice sacse0
- Gene
- PALe0
- Outcome
- most distinct DEGse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PAL (phenylalanine ammonia lyase), HCT (hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase), 4CL2 (4-coumarate: CoA ligase), C4H (cinnamate 4-hydroxylase), C3′H (p-coumarate 3-hydroxylase), and CCoAOMT1 (caffeoyl CoA 3-Omethyltransferase) were the most distinct DEGs in granulated juice sacs”
t0“Pomelo (Citrus maxima)”
Claim · model-written summary
C4H, C3′H and CCoAOMT1 were among the most distinct DEGs in granulated juice sacs.
- Taxon
- pomeloCitrus maximat0
- Organ / stage
- granulated juice sacse0
- Gene
- C4He0
- Outcome
- most distinct DEGse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C4H (cinnamate 4-hydroxylase), C3′H (p-coumarate 3-hydroxylase), and CCoAOMT1 (caffeoyl CoA 3-Omethyltransferase) were the most distinct DEGs in granulated juice sacs”
t0“Pomelo (Citrus maxima)”
Claim · model-written summary
MYB, NAC and OFP6 expression patterns were highly correlated with lignin formation.
- Taxon
- pomeloCitrus maximat0
- Gene
- MYBe0
- Outcome
- lignin formatione0
- Direction
- highly correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression patterns of several transcription factors such as MYB, NAC, OFP6, and bHLH130 are highly correlated with lignin formation”
t0“Pomelo (Citrus maxima)”
Claim · model-written summary
bHLH130 expression pattern was highly correlated with lignin formation.
- Taxon
- pomeloCitrus maximat0
- Gene
- bHLH130e0
- Outcome
- lignin formatione0
- Direction
- highly correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bHLH130 are highly correlated with lignin formation”
t0“Pomelo (Citrus maxima)”
Claim · model-written summary
qRT-PCR expression patterns were highly concordant with RNA-seq results.
- Taxon
- pomeloCitrus maximat0
- Outcome
- results were highly concordante0
- Direction
- highly concordante0
- Comparator
- RNA-seq resultse0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression patterns of the DEGs related to lignin biosynthesis and transcription factors were validated by qRT-PCR, and the results were highly concordant with the RNA-seq results”
t0“Pomelo (Citrus maxima)”