Abstract
In vitro germplasm conservation is one of the main axes in the date palm (Phoinex dactylifera) micropropagation protocol. In the present study, a set of slow growth storage treatments planned by using the manipulation of the MS salts strength, besides adding the abscisic acid (ABA) growth retardant, to the storage culture media of tow regenerative in vitro germplasm materials, i.e., the somatic embryo and the shoot cultures, of the date palm Gondelah cultivar. The investigated study conducted by observing the growth ability under the conservation conditions, the survival and the recovery growth, after the conservation period for 18 months at 15 C of the minimal growth conditions, the results revealed that half-MS strength medium, supplemented with ABA growth retardant at 8 mg/l, was the best to sustain the slow growth storage and the recovery of the date palm somatic embryo clusters explants. On the other hand, the full-MS medium supplemented with ABA growth retardant at 8 mg/l was most suitable for the in vitro preservation and the recovery of the shoot clusters of the date palm, Gondelah cultivar. Where, all recovered explants could regenerate as full intact plantlets, and successfully transferred to the acclimatization stage, to be available for the commercial or research purposes.
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Claims 3
Claim · model-written summary
Half-strength MS medium with 8 mg/l ABA was best for slow storage and recovery of date palm somatic embryo clusters.
- Taxon
- date palm
Phoenix dactyliferae0 - Organ / stage
- somatic embryo clusters explantse0
- Treatment
- half-MS strength medium, supplemented with ABA growth retardant at 8 mg/le0
- Outcome
- slow growth storage and the recoverye0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“half-MS strength medium, supplemented with ABA growth retardant at 8 mg/l, was the best to sustain the slow growth storage and the recovery of the date palm somatic embryo clusters explants”
Claim · model-written summary
Full-strength MS medium with 8 mg/l ABA was most suitable for preservation and recovery of Gondelah date palm shoot clusters.
- Taxon
- date palmPhoenix dactyliferae0
- Material
- Gondelah cultivare0
- Organ / stage
- shoot clusterse0
- Treatment
- full-MS medium supplemented with ABA growth retardant at 8 mg/le0
- Outcome
- in vitro preservation and the recoverye0
- Direction
- most suitablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“full-MS medium supplemented with ABA growth retardant at 8 mg/l was most suitable for the in vitro preservation and the recovery of the shoot clusters of the date palm, Gondelah cultivar”
Claim · model-written summary
All recovered date palm explants regenerated intact plantlets and transferred successfully to acclimatization.
- Organ / stage
- recovered explantse0
- Outcome
- regenerate as full intact plantletse0
- Qualifier
- successfully transferred to the acclimatization stagee0
Not reportedTaxon
Verbatim
e0“all recovered explants could regenerate as full intact plantlets, and successfully transferred to the acclimatization stage”
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