Abstract
Nicotine biosynthesis in tobacco callus tissues was investigated by comparing high nicotine producing strain OMT-53 with scarcely producing strain NT–5. NT–5 strain which had been subcultured for about 5 years with a medium containing 1.0 ppm 2,4-D, produced very little amount of nicotine even after more than 47 subcultures on OT–23 medium containing 0.15 ppm α-NAA. On the other hand, OMT–53 strain maintained a constant high level nicotine producing (ca. 2% or more on D.W. basis) capacity over 50 successive cultures on the same medium. The growth response of NT–5 callus tissues to α-NAA concentration was quite different from that of OMT–53. Nicotine production assumed more distinctive patterns with these two strains. NT–5 strain never produced nicotine at any concentrations of α-NAA examined, whilst OMT–53 strain produced abundant amount of nicotine within a narrow range of α-NAA concentration having the optimal value of 0.15 ppm. Nicotine supplemented to culture medium could not be decomposed either with NT–5 or with OMT–53 callus tissues. The nicotine production by OMT–53 was not affected at moderate level (1 mM) of supplemented nicotine, but at a very high level (5 mm) of the supplemented nicotine, the nicotine production seemed to have been affected considerably. Some precursors supplemented to NT–5 callus cultures did not stimulate the nicotine biosynthesis at all, even though slightly positive effect was recorded with OMT–53 callus cultures. NT–5 callus tissues had much smaller pool size of total free amino acids than OMT–53. The ratio of each amino acid in total free amino acids was not so different between both callus tissues. On the basis of these results, some discussions were made on nicotine biosynthesis in tobacco tissue cultures.
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Claims 13
Claim · model-written summary
NT–5 produced very little nicotine after more than 47 subcultures on OT–23 medium.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Material
- NT–5e0
- Outcome
- nicotinee0
- Direction
- very littlee0
- Qualifier
- more than 47 subculturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NT–5 strain which had been subcultured for about 5 years with a medium containing 1.0 ppm 2,4-D, produced very little amount of nicotine even after more than 47 subcultures on OT–23 medium containing 0.15 ppm α-NAA”
t0“Tobacco Callus Tissues”
Claim · model-written summary
OMT–53 retained high nicotine production across 50 cultures.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- OMT–53e0
- Outcome
- nicotine producinge0
- Direction
- high levele0
- Value
- ca. 2% or more on D.W. basise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OMT–53 strain maintained a constant high level nicotine producing (ca. 2% or more on D.W. basis) capacity over 50 successive cultures on the same medium”
t0“Tobacco Callus Tissues”
Claim · model-written summary
NT–5 and OMT–53 differed in growth response to α-NAA concentration.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- NT–5e0
- Treatment
- α-NAA concentratione0
- Outcome
- growth responsee0
- Comparator
- OMT–53e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth response of NT–5 callus tissues to α-NAA concentration was quite different from that of OMT–53”
t0“Tobacco Callus Tissues”
Claim · model-written summary
NT–5 produced no nicotine at any tested α-NAA concentration.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- NT–5e0
- Treatment
- α-NAAe0
- Outcome
- nicotinee0
- Direction
- never producede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NT–5 strain never produced nicotine at any concentrations of α-NAA examined”
t0“Tobacco Callus Tissues”
Claim · model-written summary
OMT–53 produced abundant nicotine within a narrow α-NAA range, optimal at 0.15 ppm.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- OMT–53e0
- Treatment
- α-NAA concentratione0
- Outcome
- nicotinee0
- Direction
- abundante0
- Value
- 0.15 ppme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OMT–53 strain produced abundant amount of nicotine within a narrow range of α-NAA concentration having the optimal value of 0.15 ppm”
t0“Tobacco Callus Tissues”
Claim · model-written summary
NT–5 callus did not decompose added nicotine.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- NT–5e0
- Treatment
- Nicotine supplemented to culture mediume0
- Outcome
- Nicotinee0
- Direction
- could not be decomposede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotine supplemented to culture medium could not be decomposed either with NT–5 or with OMT–53 callus tissues”
t0“Tobacco Callus Tissues”
Claim · model-written summary
OMT–53 callus did not decompose added nicotine.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- OMT–53e0
- Treatment
- Nicotine supplemented to culture mediume0
- Outcome
- Nicotinee0
- Direction
- could not be decomposede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotine supplemented to culture medium could not be decomposed either with NT–5 or with OMT–53 callus tissues”
t0“Tobacco Callus Tissues”
Claim · model-written summary
Moderate added nicotine did not affect OMT–53 nicotine production.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- OMT–53e0
- Treatment
- 1 mM) of supplemented nicotinee0
- Outcome
- nicotine productione0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nicotine production by OMT–53 was not affected at moderate level (1 mM) of supplemented nicotine”
t0“Tobacco Callus Tissues”
Claim · model-written summary
Very high added nicotine appeared to considerably affect OMT–53 nicotine production.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- OMT–53e1
- Treatment
- 5 mm) of the supplemented nicotinee0
- Outcome
- nicotine productione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at a very high level (5 mm) of the supplemented nicotine, the nicotine production seemed to have been affected considerably”
e1“OMT–53 was not affected”
t0“Tobacco Callus Tissues”
Claim · model-written summary
Added precursors did not stimulate NT–5 nicotine biosynthesis.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- NT–5e0
- Treatment
- Some precursorse0
- Outcome
- nicotine biosynthesise0
- Direction
- did not stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some precursors supplemented to NT–5 callus cultures did not stimulate the nicotine biosynthesis at all”
t0“Tobacco Callus Tissues”
Claim · model-written summary
Added precursors had a slight positive effect in OMT–53 callus.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- OMT–53e0
- Treatment
- Some precursorse1
- Outcome
- positive effecte0
- Direction
- slightly positivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“slightly positive effect was recorded with OMT–53 callus cultures”
e1“Some precursors supplemented to NT–5 callus cultures”
t0“Tobacco Callus Tissues”
Claim · model-written summary
NT–5 had a much smaller total free amino acid pool than OMT–53.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- NT–5e0
- Outcome
- pool size of total free amino acidse0
- Direction
- much smallere0
- Comparator
- OMT–53e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NT–5 callus tissues had much smaller pool size of total free amino acids than OMT–53”
t0“Tobacco Callus Tissues”
Claim · model-written summary
Individual amino acid proportions were similar between NT–5 and OMT–53 callus.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- NT–5e1
- Outcome
- ratio of each amino acid in total free amino acidse0
- Direction
- not so differente0
- Comparator
- OMT–53e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ratio of each amino acid in total free amino acids was not so different between both callus tissues”
e1“NT–5 callus tissues had much smaller pool size of total free amino acids than OMT–53”
t0“Tobacco Callus Tissues”
The same questions in other papers
1-naphthaleneacetic acid · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
4 papers more in this group
See all 8 papers →nicotine · yield · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.