Abstract
Date palm (Phoenix dactylifera L.) is one of the most economically important fruit crops in many Arab countries. Recently, date palm cv. KL1 was developed and subsequently cultivated in many parts of Thailand. However, male and female trees could not be clearly distinguished until trees were flowering, and it should be propagated by tissue culture to obtain true-to-type plants. This study investigated the propagation of cv. KL1 female plants using a tissue culture technique. Offshoots aged 2 yr from mother plants were selected and surface sterilized in 0.1% mercuric chloride and 1% sodium hypochlorite. The dissected shoots were then cultured on modified Murashige and Skoog (MS) medium supplemented with 100 mg/L 2,4 dichlorophenoxyacetic acid (2,4-D) and 3 mg/L kinetin and 1 mg/L 6-benzylaminopurine (BAP), then cultured under dark conditions. Initiated callus formation was observed after 7 mth of culture. The embryogenic callus cultured in MS liquid medium supplemented with 6 mg/L 2,4-D produced the most somatic (2.59 g) growth at 1 mth. The somatic embryos were proliferated to new plantlets on MS medium, until 5 cm tall. In the final stage, root induction and elongation on MS medium supplemented with 0.9 mg/L 1-naphthaleneacetic acid induced the maximum number of roots (5.2) at 2 mth.
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Claims 4
Claim · model-written summary
Callus formation was observed after 7 months of culture of date palm cv. KL1.
- Taxon
- date palm
Phoenix dactyliferae0 - Material
- cv. KL1e0
- Organ / stage
- calluse1
- Outcome
- callus formatione1
- Value
- 7 mthe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recently, date palm cv. KL1 was developed and subsequently cultivated in many parts of Thailand”
e1“Initiated callus formation was observed after 7 mth of culture”
Claim · model-written summary
Embryogenic callus in MS liquid medium with 6 mg/L 2,4-D produced the most somatic growth at 1 month.
- Taxon
- date palmPhoenix dactyliferae0
- Material
- cv. KL1e0
- Organ / stage
- embryogenic calluse2
- Treatment
- MS liquid medium supplemented with 6 mg/L 2,4-De2
- Outcome
- growthe2
- Direction
- the moste2
- Value
- 2.59 ge2
- Qualifier
- at 1 mthe2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recently, date palm cv. KL1 was developed and subsequently cultivated in many parts of Thailand”
e2“The embryogenic callus cultured in MS liquid medium supplemented with 6 mg/L 2,4-D produced the most somatic (2.59 g) growth at 1 mth”
Claim · model-written summary
Somatic embryos proliferated to new plantlets on MS medium until 5 cm tall.
- Taxon
- date palmPhoenix dactyliferae0
- Material
- cv. KL1e0
- Organ / stage
- somatic embryose3
- Treatment
- MS mediume3
- Outcome
- proliferated to new plantletse3
- Value
- 5 cm talle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recently, date palm cv. KL1 was developed and subsequently cultivated in many parts of Thailand”
e3“The somatic embryos were proliferated to new plantlets on MS medium, until 5 cm tall”
Claim · model-written summary
Root induction on MS medium with 0.9 mg/L 1-naphthaleneacetic acid gave the maximum number of roots (5.2) at 2 months.
- Taxon
- date palmPhoenix dactyliferae0
- Material
- cv. KL1e0
- Treatment
- 0.9 mg/L 1-naphthaleneacetic acide4
- Outcome
- number of rootse4
- Direction
- maximume4
- Value
- 5.2e4
- Qualifier
- at 2 mthe4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recently, date palm cv. KL1 was developed and subsequently cultivated in many parts of Thailand”
e4“root induction and elongation on MS medium supplemented with 0.9 mg/L 1-naphthaleneacetic acid induced the maximum number of roots (5.2) at 2 mth”
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