Abstract
Lipoxygenase-3, the major component of the enzyme in rice grain, was purified 2980-fold with a yield of 7% from embryos. The purified enzyme had a specific activity of 280 μmol O2 formed/min per mg protein. This enzyme was inactivated by SH compounds, such as cysteine and glutathione. The inactivation was prevented by the addition of catalase or replacement of the air by N2 gas. These two treatments were also effective for the stable storage of the purified enzyme. The molecular weights measured by sodium dodecyl sulfate gel and gradient gel electrophoresis were 93, 000 and 89, 000, respectively, indicating that the enzyme is a single polypeptide chain. The purified enzyme contained 0.73 Fe atom per molecule. The absorption spectrum suggested that the enzyme is a non-heme iron protein. Some similarities in amino-acid composition were observed between rice, soybean, and pea lipoxygenases. The purified enzyme specifically produced 9-Dhydroperoxy-10, 12(E, Z)-octadecadienoic acid when linoleic acid was used as a substrate.
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Claims 14
Claim · model-written summary
Rice embryo lipoxygenase-3 was purified 2980-fold with 7% yield.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- embryose0
- Gene
- Lipoxygenase-3e0
- Outcome
- purifiede0
- Value
- 2980-fold with a yield of 7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“Lipoxygenase-3, the major component of the enzyme in rice grain, was purified 2980-fold with a yield of 7% from embryos.”
Claim · model-written summary
Purified lipoxygenase-3 had specific activity of 280 μmol O2 formed/min per mg protein.
- Taxon
- riceOryza sativat0
- Gene
- lipoxygenase component 3t0
- Outcome
- specific activitye0
- Value
- 280 μmol O2 formed/min per mg proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The purified enzyme had a specific activity of 280 μmol O2 formed/min per mg protein.”
Claim · model-written summary
SH compounds including cysteine and glutathione inactivated the enzyme.
- Taxon
- riceOryza sativat0
- Treatment
- SH compounds, such as cysteine and glutathionee0
- Gene
- lipoxygenase component 3t0
- Outcome
- enzymee0
- Direction
- inactivatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“This enzyme was inactivated by SH compounds, such as cysteine and glutathione.”
Claim · model-written summary
Catalase addition prevented inactivation of the rice enzyme.
- Taxon
- riceOryza sativat0
- Treatment
- addition of catalasee0
- Gene
- lipoxygenase component 3t0
- Outcome
- inactivatione0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The inactivation was prevented by the addition of catalase or replacement of the air by N2 gas.”
Claim · model-written summary
Replacing air with N2 prevented inactivation of the rice enzyme.
- Taxon
- riceOryza sativat0
- Treatment
- replacement of the air by N2 gase0
- Gene
- lipoxygenase component 3t0
- Outcome
- inactivatione0
- Direction
- preventede0
- Comparator
- aire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The inactivation was prevented by the addition of catalase or replacement of the air by N2 gas.”
Claim · model-written summary
Catalase addition enabled stable storage of the purified enzyme.
- Taxon
- riceOryza sativat0
- Treatment
- addition of catalasee0
- Outcome
- stable storagee0
- Direction
- effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The inactivation was prevented by the addition of catalase or replacement of the air by N2 gas. These two treatments were also effective for the stable storage of the purified enzyme.”
Claim · model-written summary
Replacing air with N2 enabled stable storage of the purified enzyme.
- Taxon
- riceOryza sativat0
- Treatment
- replacement of the air by N2 gase0
- Outcome
- stable storagee0
- Direction
- effectivee0
- Comparator
- aire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The inactivation was prevented by the addition of catalase or replacement of the air by N2 gas. These two treatments were also effective for the stable storage of the purified enzyme.”
Claim · model-written summary
SDS gel electrophoresis measured the enzyme molecular weight as 93, 000.
- Taxon
- riceOryza sativat0
- Outcome
- molecular weightse0
- Value
- 93, 000e0
- Qualifier
- sodium dodecyl sulfate gele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The molecular weights measured by sodium dodecyl sulfate gel and gradient gel electrophoresis were 93, 000 and 89, 000, respectively, indicating that the enzyme is a single polypeptide chain.”
Claim · model-written summary
Gradient gel electrophoresis measured the enzyme molecular weight as 89, 000.
- Taxon
- riceOryza sativat0
- Outcome
- molecular weightse0
- Value
- 89, 000e0
- Qualifier
- gradient gel electrophoresise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The molecular weights measured by sodium dodecyl sulfate gel and gradient gel electrophoresis were 93, 000 and 89, 000, respectively, indicating that the enzyme is a single polypeptide chain.”
Claim · model-written summary
The enzyme was inferred to be a single polypeptide chain.
- Taxon
- riceOryza sativat0
- Outcome
- single polypeptide chaine0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“indicating that the enzyme is a single polypeptide chain.”
Claim · model-written summary
The purified enzyme contained 0.73 Fe atom per molecule.
- Taxon
- riceOryza sativat0
- Outcome
- Fe atom per moleculee0
- Value
- 0.73 Fe atom per moleculee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The purified enzyme contained 0.73 Fe atom per molecule.”
Claim · model-written summary
Absorption spectra suggested the enzyme is a non-heme iron protein.
- Taxon
- riceOryza sativat0
- Outcome
- non-heme iron proteine0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The absorption spectrum suggested that the enzyme is a non-heme iron protein.”
Claim · model-written summary
Rice, soybean and pea lipoxygenases had some similar amino-acid composition.
- Taxon
- riceOryza sativae0
- Gene
- lipoxygenasese0
- Outcome
- amino-acid compositione0
- Direction
- similaritiese0
- Comparator
- soybean, and pea lipoxygenasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“Some similarities in amino-acid composition were observed between rice, soybean, and pea lipoxygenases.”
Claim · model-written summary
With linoleic acid substrate the rice enzyme specifically produced 9-Dhydroperoxy-10, 12(E, Z)-octadecadienoic acid.
- Taxon
- riceOryza sativat0
- Outcome
- 9-Dhydroperoxy-10, 12(E, Z)-octadecadienoic acide0
- Qualifier
- linoleic acid was used as a substratee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Purification and characterization of rice lipoxygenase component 3 from embryos.”
e0“The purified enzyme specifically produced 9-Dhydroperoxy-10, 12(E, Z)-octadecadienoic acid when linoleic acid was used as a substrate.”
The same questions in other papers
glutathione · physiology: enzyme activity · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →cysteine · physiology: enzyme activity · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.