Abstract
Nine fructo-oligosaccharides, synthesized in vitro from sucrose by an enzyme preparation from asparagus roots, were isolated and their structures were elucidated to be 1F (1-β-fructofuranosyl)n sucrose [n = 1 (1-kestose), 2 (nystose) and 3], 6G (1-β-fructofuranosyl)n sucrose [n=1 (neokestose), 2 and 3] and 1F (1-β-fructofuranosyl)m-6G (1-β-fructofuranosyl)n sucrose [m=1, n=1; m=2, n =1; and m =1, n=2]. These saccharides are all known to occur naturally in asparagus roots, but 6G (1-β-fructofuranosyl)3 sucrose and 1F (1-β-fructofuranosyl)m-6G-(1-β-fructofuranosyl)n sucrose (m=1, n =1; and m=1, n=2) were the first saccharides enzymatically synthesized in vitro. Also three types of fructosyltransferases were presumed to be involved in the biosynthesis of these oligosaccharides in asparagus roots.
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Claims 3
Claim · model-written summary
Nine fructo-oligosaccharides synthesized by an asparagus-root enzyme preparation were isolated and structurally elucidated.
AsparagusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nine fructo-oligosaccharides, synthesized in vitro from sucrose by an enzyme preparation from asparagus roots, were isolated and their structures were elucidated to be 1F (1-β-fructofuranosyl)n sucrose [n = 1 (1-kestose), 2 (nystose) and 3], 6G (1-β-fructofuranosyl)n sucrose [n=1 (neokestose), 2 and 3] and 1F (1-β-fructofuranosyl)m-6G (1-β-fructofuranosyl)n sucrose [m=1, n=1; m=2, n =1; and m =1, n=2].”
Claim · model-written summary
Three specified saccharides were reported as the first enzymatically synthesized in vitro.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These saccharides are all known to occur naturally in asparagus roots, but 6G (1-β-fructofuranosyl)3 sucrose and 1F (1-β-fructofuranosyl)m-6G-(1-β-fructofuranosyl)n sucrose (m=1, n =1; and m=1, n=2) were the first saccharides enzymatically synthesized in vitro.”
Claim · model-written summary
Three types of fructosyltransferases were presumed to participate in oligosaccharide biosynthesis in asparagus roots.
- Organ / stage
- rootse0
- Gene
- fructosyltransferasese0
- Outcome
- biosynthesis of these oligosaccharidese0
- Value
- three typese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Also three types of fructosyltransferases were presumed to be involved in the biosynthesis of these oligosaccharides in asparagus roots.”