Abstract
Muscadine grape ( Vitis rotundifolia ) is highly resistant to many diseases and insects that attack european grape ( Vitis vinifera ). However, distant hybridization incompatibility between V . rotundifolia (female) and V . vinifera (male) impedes the utilization of V . rotundifolia in grape breeding. This study used fourth-dimension label-free protein quantitation to detect the key genes and pathways in the V . rotundifolia stigma after self-pollination ( V . rotundifolia × V . rotundifolia ) and cross-pollination ( V . rotundifolia × V . vinifera ). A histological analysis showed that pollen tube growth in the stigma of V . rotundifolia was arrested 8 hours after cross-pollination, but not after self-pollination. A proteomic analysis identified 32 differentially expressed proteins (DEPs) in the stigma of V . rotundifolia between self-pollination and cross-pollination. A heatmap analysis grouped these DEPs into four clusters. The top gene ontology terms were ATPase-coupled transmembrane transporter activity, extracellular region, DNA replication, and cellular carbohydrate biosynthetic process. A Kyoto Encyclopedia of Genes and Genomes analysis revealed that these DEPs participated in DNA replication and starch and sucrose metabolism pathways. The downregulated A5AY88, D7TJ35, D7SU26, F6HJI1, and F6GUE7 may have a role in cross incompatibility. This study revealed the cross incompatibility of grapes at histological and proteomic levels.
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Claims 6
Claim · model-written summary
Pollen tube growth in the V. rotundifolia stigma was arrested 8 hours after cross-pollination but not after self-pollination.
- Taxon
- V . rotundifolia
Vitis rotundifoliae0 - Organ / stage
- stigmae0
- Treatment
- cross-pollinatione0
- Outcome
- pollen tube growthe0
- Direction
- arrestede0
- Qualifier
- 8 hourse0
- Comparator
- self-pollinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pollen tube growth in the stigma of V . rotundifolia was arrested 8 hours after cross-pollination, but not after self-pollination”
Claim · model-written summary
A proteomic analysis identified 32 differentially expressed proteins in the V. rotundifolia stigma between self-pollination and cross-pollination.
- Taxon
- V . rotundifoliaVitis rotundifoliae0
- Organ / stage
- stigmae0
- Treatment
- cross-pollinatione0
- Outcome
- differentially expressed proteinse0
- Value
- 32e0
- Comparator
- self-pollinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A proteomic analysis identified 32 differentially expressed proteins (DEPs) in the stigma of V . rotundifolia between self-pollination and cross-pollination”
Claim · model-written summary
The top gene ontology terms among the DEPs included ATPase-coupled transmembrane transporter activity and extracellular region.
- Taxon
- Vitis rotundifoliaVitis rotundifoliat0
- Outcome
- top gene ontology termse0
- Qualifier
- ATPase-coupled transmembrane transporter activity, extracellular regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The top gene ontology terms were ATPase-coupled transmembrane transporter activity, extracellular region”
t0“Vitis rotundifolia”
Claim · model-written summary
The top gene ontology terms among the DEPs also included DNA replication and cellular carbohydrate biosynthetic process.
- Taxon
- Vitis rotundifoliaVitis rotundifoliat0
- Outcome
- cellular carbohydrate biosynthetic processe0
- Qualifier
- DNA replicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA replication, and cellular carbohydrate biosynthetic process”
t0“Vitis rotundifolia”
Claim · model-written summary
A KEGG analysis showed the DEPs participated in DNA replication and starch and sucrose metabolism pathways.
- Taxon
- Vitis rotundifoliaVitis rotundifoliat0
- Outcome
- DNA replication and starch and sucrose metabolism pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these DEPs participated in DNA replication and starch and sucrose metabolism pathways”
t0“Vitis rotundifolia”
Claim · model-written summary
The downregulated proteins A5AY88, D7TJ35, D7SU26, F6HJI1 and F6GUE7 may have a role in cross incompatibility.
- Taxon
- Vitis rotundifoliaVitis rotundifoliat0
- Gene
- A5AY88, D7TJ35, D7SU26, F6HJI1, and F6GUE7e0
- Outcome
- cross incompatibilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The downregulated A5AY88, D7TJ35, D7SU26, F6HJI1, and F6GUE7 may have a role in cross incompatibility”
t0“Vitis rotundifolia”