Abstract
Dwarfism is a beneficial trait in many crops. Dwarf crops hold certain advantages over taller crops in lodging resistance, fertilizer tolerance, and yield. Overexpression of CBF/DREB transcription factors can lead to dwarfing in many plant species, but the molecular mechanism of plant dwarfing caused by overexpression of CBF/DREB in upland cotton ( Gossypium hirsutum ) remains unclear. In this study, we observed that overexpression of the Ammopiptanthus mongolicus AmCBF1 transcription factor in upland cotton R15 reduced plant height, whereas virus-induced gene silencing of AmCBF1 in the derived dwarf lines L28 and L30 partially restored plant height. Five protein phosphatase (PP2C) genes ( GhPP2C1 to GhPP2C5 ) in cotton were identified by RNA-sequencing among genes differentially expressed in L28 or L30 in comparison with R15 and thus may play an important role in AmCBF1-regulated dwarfing in cotton. Gene expression analysis showed that the GhPP2C genes were down-regulated significantly in L28 and L30, and silencing of GhPP2C1 or GhPP2C2 in R15 inhibited the growth of cotton seedlings. Subcellular localization assays revealed that GhPP2C1 was localized to the cell membrane and nucleus, whereas GhPP2C2 was exclusively localized to the nucleus. Yeast one-hybrid and dual-luciferase assays showed that AmCBF1 was able to bind to the CRT/DRE elements of the upstream promoter of GhPP2C1 or GhPP2C2 and repress their expression. These findings provide insight into the mechanism of dwarfing and may contribute to the breeding of dwarf cultivars of upland cotton.
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Claims 10
Claim · model-written summary
AmCBF1 overexpression reduced cotton height.
- Taxon
- upland cottone0
- Material
- R15e0
- Treatment
- overexpressione0
- Gene
- AmCBF1e0
- Outcome
- plant heighte0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“overexpression of the Ammopiptanthus mongolicus AmCBF1 transcription factor in upland cotton R15 reduced plant height”
Claim · model-written summary
Silencing AmCBF1 partly restored height in dwarf lines.
- Taxon
- Gossypium hirsutum
Gossypium hirsutumt0 - Material
- L28 and L30e0
- Treatment
- virus-induced gene silencing of AmCBF1e0
- Gene
- AmCBF1e0
- Outcome
- plant heighte0
- Direction
- restorede0
- Qualifier
- partiallye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus-induced gene silencing of AmCBF1 in the derived dwarf lines L28 and L30 partially restored plant height”
t0“Gossypium hirsutum”
Claim · model-written summary
Five GhPP2C genes were identified as differentially expressed in dwarf lines.
- Taxon
- cottonGossypium hirsutume0
- Material
- L28 or L30e0
- Gene
- GhPP2C1 to GhPP2C5e0
- Outcome
- genes differentially expressede0
- Value
- Fivee0
- Comparator
- R15e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five protein phosphatase (PP2C) genes ( GhPP2C1 to GhPP2C5 ) in cotton were identified by RNA-sequencing among genes differentially expressed in L28 or L30 in comparison with R15”
Claim · model-written summary
GhPP2C expression was significantly lower in dwarf lines.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Material
- L28 and L30e0
- Gene
- GhPP2C genese0
- Outcome
- GhPP2C genese0
- Direction
- down-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the GhPP2C genes were down-regulated significantly in L28 and L30”
t0“Gossypium hirsutum”
Claim · model-written summary
GhPP2C1 silencing inhibited seedling growth.
- Taxon
- cottonGossypium hirsutume0
- Material
- R15e0
- Treatment
- silencing of GhPP2C1e0
- Gene
- GhPP2C1e0
- Outcome
- growth of cotton seedlingse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of GhPP2C1 or GhPP2C2 in R15 inhibited the growth of cotton seedlings”
Claim · model-written summary
GhPP2C2 silencing inhibited seedling growth.
- Taxon
- cottonGossypium hirsutume0
- Material
- R15e0
- Treatment
- GhPP2C2e0
- Gene
- GhPP2C2e0
- Outcome
- growth of cotton seedlingse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of GhPP2C1 or GhPP2C2 in R15 inhibited the growth of cotton seedlings”
Claim · model-written summary
GhPP2C1 localized to membrane and nucleus.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- GhPP2C1e0
- Outcome
- localized to the cell membrane and nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhPP2C1 was localized to the cell membrane and nucleus”
t0“Gossypium hirsutum”
Claim · model-written summary
GhPP2C2 localized only to nucleus.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- GhPP2C2e0
- Outcome
- exclusively localized to the nucleuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhPP2C2 was exclusively localized to the nucleus”
t0“Gossypium hirsutum”
Claim · model-written summary
AmCBF1 bound GhPP2C1 promoter and repressed expression.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- GhPP2C1e0
- Outcome
- repress their expressione0
- Direction
- represse0
- Qualifier
- bind to the CRT/DRE elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AmCBF1 was able to bind to the CRT/DRE elements of the upstream promoter of GhPP2C1 or GhPP2C2 and repress their expression”
t0“Gossypium hirsutum”
Claim · model-written summary
AmCBF1 bound GhPP2C2 promoter and repressed expression.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- GhPP2C2e0
- Outcome
- repress their expressione0
- Direction
- represse0
- Qualifier
- bind to the CRT/DRE elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AmCBF1 was able to bind to the CRT/DRE elements of the upstream promoter of GhPP2C1 or GhPP2C2 and repress their expression”
t0“Gossypium hirsutum”
The same questions in other papers
loss of function · growth · Gossypium hirsutum (cotton)Positive and negative results
8 papers in the same direction as this paper · 25 in the opposite direction
- positive
- no effect
- negative
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See all 34 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.