Abstract
Cotton architecture is partly determined by shoot branching and flowering patterns. GhBRC1 was previously identified by RNA-seq analysis of nulliplex-branching and normal-branching cotton. However, the roles of GhBRC1 in cotton remain unclear. In the present study, investigations of nuclear localization and transcriptional activity indicated that GhBRC1 has characteristics typical of transcription factors. Gene expression analysis showed that GhBRC1 was highly expressed in axillary buds but displayed different expression patterns between the two branching types. Overexpression of GhBRC1 in Arabidopsis significantly inhibited the number of branches and promoted flowering. In contrast, silencing GhBRC1 in cotton significantly promoted seedling growth. GhBRC1 was induced by multiple hormones, including strigolactones, which promoted seedling growth and seed germination of Arabidopsis plants overexpressing GhBRC1. Consistent with these findings, RNA-seq analysis of virus-induced gene silencing treated cotton revealed that a large number of genes were differentially expressed between GhBRC1-silenced and control plants, and these genes were significantly enriched in plant hormone signalling pathways. Together, our data indicates that GhBRC1 regulates plant branching and flowering through multiple regulatory pathways, especially those regulating plant hormones, with functions partly differing from those of Arabidopsis BRC1. These results provide insights into the molecular mechanisms controlling plant architecture, which is important for breeding cotton with ideal plant architecture and high yield.
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Claims 10
Claim · model-written summary
GhBRC1 has transcription factor characteristics.
- Taxon
- cotton
Gossypium hirsutume0t0 - Gene
- GhBRC1e0
- Outcome
- characteristics typical of transcription factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhBRC1 has characteristics typical of transcription factors”
t0“Cotton”
Claim · model-written summary
GhBRC1 was highly expressed in cotton axillary buds, with expression differing between branching types.
- Taxon
- cottonGossypium hirsutume0t0
- Organ / stage
- axillary budse0
- Gene
- GhBRC1e0
- Outcome
- different expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhBRC1 was highly expressed in axillary buds but displayed different expression patterns between the two branching types”
t0“Cotton”
Claim · model-written summary
GhBRC1 overexpression reduced Arabidopsis branch number.
- Taxon
- Arabidopsis
Arabidopsise0t0 - Treatment
- Overexpression of GhBRC1e0
- Gene
- GhBRC1e0
- Outcome
- number of branchese0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GhBRC1 in Arabidopsis significantly inhibited the number of branches”
t0“Cotton”
Claim · model-written summary
GhBRC1 overexpression promoted flowering in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0t0
- Treatment
- Overexpression of GhBRC1e0
- Gene
- GhBRC1e0
- Outcome
- floweringe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GhBRC1 in Arabidopsis significantly inhibited the number of branches and promoted flowering”
t0“Cotton”
Claim · model-written summary
Silencing GhBRC1 promoted cotton seedling growth.
- Taxon
- cottonGossypium hirsutume0t0
- Treatment
- silencing GhBRC1e0
- Gene
- GhBRC1e0
- Outcome
- seedling growthe0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing GhBRC1 in cotton significantly promoted seedling growth”
t0“Cotton”
Claim · model-written summary
Multiple hormones including strigolactones induced GhBRC1.
- Taxon
- cottonGossypium hirsutume0t0
- Gene
- GhBRC1e0
- Compound
- strigolactonese0
- Outcome
- induced by multiple hormonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhBRC1 was induced by multiple hormones, including strigolactones”
t0“Cotton”
Claim · model-written summary
Strigolactones promoted seedling growth and germination in Arabidopsis overexpressing GhBRC1.
- Taxon
- ArabidopsisArabidopsise0t0
- Treatment
- strigolactonese0
- Gene
- GhBRC1e0
- Outcome
- seedling growth and seed germinatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strigolactones, which promoted seedling growth and seed germination of Arabidopsis plants overexpressing GhBRC1”
t0“Cotton”
Claim · model-written summary
Many genes differed in expression between GhBRC1-silenced and control cotton.
- Taxon
- cottonGossypium hirsutume0t0
- Gene
- GhBRC1e0
- Outcome
- genes were differentially expressede0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a large number of genes were differentially expressed between GhBRC1-silenced and control plants”
t0“Cotton”
Claim · model-written summary
Differentially expressed genes were enriched in plant hormone signaling pathways.
- Taxon
- cottonGossypium hirsutume0t0
- Outcome
- enriched in plant hormone signalling pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these genes were significantly enriched in plant hormone signalling pathways”
t0“Cotton”
Claim · model-written summary
The authors infer that GhBRC1 regulates branching and flowering through hormone pathways.
Verbatim
e0“GhBRC1 regulates plant branching and flowering through multiple regulatory pathways, especially those regulating plant hormones”
t0“Cotton”
The same questions in other papers
loss of function · growth · Gossypium hirsutum (cotton)Positive and negative results
8 papers in the same direction as this paper · 25 in the opposite direction
- positive
- no effect
- negative
30 papers more in this group
See all 34 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.