Paper · 2022 · BIO Web of Conferences

Evaluation of sensitivity of apple scab pathogen to difenoconazole using the discriminatory dose technique

Andrey Nasonov; Galina Yakuba; Nikita Marchenko; et al.

6
claims
16
verbatim quotes
6
citations
3
research areas
✓
Open access · cc-by

Abstract

The most serious disease of the apple tree in all areas of its growth is scab. In the integrated apple tree protection system, the main method is chemical. However, the use of chemical fungicides is characterized by the risk of developing resistance to them by pathogen. The sensitivity of 118 monospore isolates of Venturia inaequalis was studied from three orchards of Jeromine, Reinette Simirenko and Gala cultivars, differing in the frequency of application of difenoconazole. Sensitivity was determined using the discriminatory dose technique (0.01 mg/l of active substance) in terms of RG, the relative growth of the mycelium. RG was expressed as the degree of change in mycelium growth in a nutrient medium with fungicide relative to the control variant in percent. All pathogen populations studied differed significantly in mean RG values. Populations treated three times per season with difenoconazole had higher RG values compared to populations treated two times. From Gala orchard, for some isolates, a stimulating effect of a discriminatory dose of difenoconazole on their growth was observed, that is, a hormesis effect was manifested. The proportion of isolates with RG values above the cutoff value, which was 84 and 100 % for the Reinette Simirenko and Gala orchards, may indicate that the pathogen populations studied are resistant, and in these orchards, there may be a decrease in the effectiveness of protection against apple scab. The discriminatory dose technique allowed us to objectively and promptly assess the sensitivity of V. inaequalis populations from orchards with varying intensity use of difenoconazole.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 6

Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Venturia inaequalis populations from the three apple orchards differed significantly in mean RG values

Interactor
Venturia inaequalisVenturia inaequalise0pathogen
Outcome
mean RG valuese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Venturia inaequalis
Venturia inaequalis · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

t0

“apple scab pathogen”

✓ located verbatim in the source · title, characters 29–48 · show in text ↑
e0

“The sensitivity of 118 monospore isolates of Venturia inaequalis was studied from three orchards of Jeromine, Reinette Simirenko and Gala cultivars, differing in the frequency of application of difenoconazole”

✓ located verbatim in the source · abstract, characters 271–479 · show in text ↑
e1

“All pathogen populations studied differed significantly in mean RG values”

✓ located verbatim in the source · abstract, characters 773–846 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Apple scab populations treated three times per season with difenoconazole had higher RG values than populations treated two times

Treatment
treated three times per season with difenoconazolee2
Interactor
Venturia inaequalisVenturia inaequalise0pathogen
Outcome
RG valuese2
Direction
highere2
Comparator
populations treated two timese2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Venturia inaequalis
Venturia inaequalis · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

t0

“apple scab pathogen”

✓ located verbatim in the source · title, characters 29–48 · show in text ↑
e0

“The sensitivity of 118 monospore isolates of Venturia inaequalis was studied from three orchards of Jeromine, Reinette Simirenko and Gala cultivars, differing in the frequency of application of difenoconazole”

✓ located verbatim in the source · abstract, characters 271–479 · show in text ↑
e2

“Populations treated three times per season with difenoconazole had higher RG values compared to populations treated two times”

✓ located verbatim in the source · abstract, characters 848–973 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

A discriminatory dose of difenoconazole stimulated the growth of some Venturia inaequalis isolates from Gala orchard

Treatment
discriminatory dose of difenoconazolee3
Interactor
Venturia inaequalisVenturia inaequalise0pathogen
Outcome
their growthe3
Direction
stimulating effecte3
Setting
Gala orcharde3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Venturia inaequalis
Venturia inaequalis · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

t0

“apple scab pathogen”

✓ located verbatim in the source · title, characters 29–48 · show in text ↑
e0

“The sensitivity of 118 monospore isolates of Venturia inaequalis was studied from three orchards of Jeromine, Reinette Simirenko and Gala cultivars, differing in the frequency of application of difenoconazole”

✓ located verbatim in the source · abstract, characters 271–479 · show in text ↑
e3

“From Gala orchard, for some isolates, a stimulating effect of a discriminatory dose of difenoconazole on their growth was observed”

✓ located verbatim in the source · abstract, characters 975–1105 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Proportion of isolates with RG above the cutoff value was 84 and 100 % in the Reinette Simirenko and Gala orchards

Interactor
Venturia inaequalisVenturia inaequalise0pathogen
Outcome
proportion of isolates with RG values above the cutoff valuee4
Value
84 and 100 %e4
Setting
Reinette Simirenko and Gala orchardse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Venturia inaequalis
Venturia inaequalis · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

t0

“apple scab pathogen”

✓ located verbatim in the source · title, characters 29–48 · show in text ↑
e0

“The sensitivity of 118 monospore isolates of Venturia inaequalis was studied from three orchards of Jeromine, Reinette Simirenko and Gala cultivars, differing in the frequency of application of difenoconazole”

✓ located verbatim in the source · abstract, characters 271–479 · show in text ↑
e4

“The proportion of isolates with RG values above the cutoff value, which was 84 and 100 % for the Reinette Simirenko and Gala orchards, may indicate that the pathogen populations studied are resistant, and in these orchards, there may be a decrease in the effectiveness of protection against apple scab”

✓ located verbatim in the source · abstract, characters 1150–1451 · show in text ↑
Associationhypothesisoriginal resultnegativemeasured

Claim · model-written summary

Effectiveness of apple scab protection may decrease in the Reinette Simirenko and Gala orchards

Outcome
effectiveness of protection against apple scabe4
Direction
decreasee4
Setting
Reinette Simirenko and Gala orchardse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

t0

“apple scab pathogen”

✓ located verbatim in the source · title, characters 29–48 · show in text ↑
e4

“The proportion of isolates with RG values above the cutoff value, which was 84 and 100 % for the Reinette Simirenko and Gala orchards, may indicate that the pathogen populations studied are resistant, and in these orchards, there may be a decrease in the effectiveness of protection against apple scab”

✓ located verbatim in the source · abstract, characters 1150–1451 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The discriminatory dose technique objectively and promptly assesses sensitivity of Venturia inaequalis populations

Interactor
V. inaequalisVenturia inaequalise5pathogen
Outcome
sensitivity of V. inaequalis populationse5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Venturia inaequalis
Venturia inaequalis · InteractorPublic domain · Wikimedia Commons ↗

Verbatim

t0

“apple scab pathogen”

✓ located verbatim in the source · title, characters 29–48 · show in text ↑
e5

“The discriminatory dose technique allowed us to objectively and promptly assess the sensitivity of V. inaequalis populations from orchards with varying intensity use of difenoconazole”

✓ located verbatim in the source · abstract, characters 1453–1636 · show in text ↑

difenoconazole · Venturia inaequalis · Malus domestica (apple)Positive and negative results

6 papers: 3 positive, 3 negative or no effect

2 papers in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

climate site · Venturia inaequalis · Malus domestica (apple)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. no effect
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.