Paper · 2022 · FORESTRY BULLETIN

Clonal micropropagation improvement technique of Lonicera Caerulea L. promising cultivars

N.D. Orlova; E.N. Raeva-Bogoslovskaya; O.I. Molkanova

6
claims
12
verbatim quotes
2
citations
3
research areas
✓
Open access

Abstract

The work is devoted to the optimization of clonal micropropagation method of the Lonicera caerulea L. cultivars. The influence of various components of the nutrient medium at all stages of cultivation in vitro has been established. The highest multiplication factor in varieties Diana and Yugana was noted with the addition of glucose at a concentration of 40 g/l and the addition of sucrose at a concentration of 20 g/l. When studying the effect of auxin type on the root formation of honeysuckle varieties, the highest percentage of rooting in Yugana varieties was observed on a nutrient medium with indolylbutyric acid (99 %), and in Gzhelka variety with indoleacetic acid (96 %). It was found that 200 mg/l of iron chelate (Fe(III)-EDDHA) in the composition of the nutrient medium had a positive effect on the percentage of root formation of the variety Diana 81 %. For the Yugana variety (rooting rate 76 %), it is better to use a nutrient medium with the addition of iron chelate Fe(III)-EDTA at a concentration of 73,4 mg/l.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Glucose at 40 g/l gave the highest multiplication factor in Diana.

Taxon
Lonicera caerulea L.Lonicera caeruleat0
Material
Dianae0
Treatment
glucose at a concentration of 40 g/le0
Outcome
multiplication factore0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea
Lonicera caerulea · TaxonOpioła Jerzy (Poland) · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The highest multiplication factor in varieties Diana and Yugana was noted with the addition of glucose at a concentration of 40 g/l and the addition of sucrose at a concentration of 20 g/l”

✓ located verbatim in the source · abstract, characters 232–420 · show in text ↑
t0

“Lonicera Caerulea L.”

✓ located verbatim in the source · title, characters 49–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Sucrose at 20 g/l gave the highest multiplication factor in Yugana.

Taxon
Lonicera caerulea L.Lonicera caeruleat0
Material
Yuganae0
Treatment
sucrose at a concentration of 20 g/le0
Outcome
multiplication factore0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea
Lonicera caerulea · TaxonOpioła Jerzy (Poland) · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The highest multiplication factor in varieties Diana and Yugana was noted with the addition of glucose at a concentration of 40 g/l and the addition of sucrose at a concentration of 20 g/l”

✓ located verbatim in the source · abstract, characters 232–420 · show in text ↑
t0

“Lonicera Caerulea L.”

✓ located verbatim in the source · title, characters 49–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Indolylbutyric acid produced the highest rooting percentage, 99%, in Yugana.

Taxon
Lonicera caerulea L.Lonicera caeruleat0
Material
Yuganae0
Treatment
indolylbutyric acide0
Outcome
percentage of rootinge0
Direction
higheste0
Value
99 %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea
Lonicera caerulea · TaxonOpioła Jerzy (Poland) · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“the highest percentage of rooting in Yugana varieties was observed on a nutrient medium with indolylbutyric acid (99 %)”

✓ located verbatim in the source · abstract, characters 509–628 · show in text ↑
t0

“Lonicera Caerulea L.”

✓ located verbatim in the source · title, characters 49–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Indoleacetic acid produced 96% rooting in Gzhelka.

Taxon
Lonicera caerulea L.Lonicera caeruleat0
Material
Gzhelkae0
Treatment
indoleacetic acide0
Outcome
rootinge0
Value
96 %e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea
Lonicera caerulea · TaxonOpioła Jerzy (Poland) · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“the highest percentage of rooting in Yugana varieties was observed on a nutrient medium with indolylbutyric acid (99 %), and in Gzhelka variety with indoleacetic acid (96 %)”

✓ located verbatim in the source · abstract, characters 509–682 · show in text ↑
t0

“Lonicera Caerulea L.”

✓ located verbatim in the source · title, characters 49–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Iron chelate Fe(III)-EDDHA at 200 mg/l positively affected root formation in Diana, reaching 81%.

Taxon
Lonicera caerulea L.Lonicera caeruleat0
Material
Dianae0
Treatment
200 mg/l of iron chelate (Fe(III)-EDDHA)e0
Outcome
percentage of root formatione0
Direction
positive effecte0
Value
81 %e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea
Lonicera caerulea · TaxonOpioła Jerzy (Poland) · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“200 mg/l of iron chelate (Fe(III)-EDDHA) in the composition of the nutrient medium had a positive effect on the percentage of root formation of the variety Diana 81 %”

✓ located verbatim in the source · abstract, characters 702–868 · show in text ↑
t0

“Lonicera Caerulea L.”

✓ located verbatim in the source · title, characters 49–69 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Fe(III)-EDTA at 73,4 mg/l gave a 76% rooting rate in Yugana and was recommended for its medium.

Taxon
Lonicera caerulea L.Lonicera caeruleat0
Material
Yuganae0
Treatment
iron chelate Fe(III)-EDTA at a concentration of 73,4 mg/le0
Outcome
rooting ratee0
Value
76 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea
Lonicera caerulea · TaxonOpioła Jerzy (Poland) · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“For the Yugana variety (rooting rate 76 %), it is better to use a nutrient medium with the addition of iron chelate Fe(III)-EDTA at a concentration of 73,4 mg/l”

✓ located verbatim in the source · abstract, characters 870–1030 · show in text ↑
t0

“Lonicera Caerulea L.”

✓ located verbatim in the source · title, characters 49–69 · show in text ↑

sucrose · pest · Lonicera caerulea (blue honeysuckle)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

glucose · pest · Lonicera caerulea (blue honeysuckle)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

iron atom · growth · Lonicera caerulea (blue honeysuckle)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

indole-3-acetic acid · growth · Lonicera caerulea (blue honeysuckle)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.