Abstract
Humic acid-enhanced phosphate fertilizer (HAP) is widely applied in Chinese agriculture due to its high efficiency. Although the structural composition and physicochemical properties of humic acid (HA) are significantly altered during HAP production, a clear understanding of the mechanisms underlying the biological effects of HA extracted from HAP fertilizer (PHA) on plant growth is still lacking. In the current study, we extracted PHA from HAP and assessed its effects on the dry biomass, phosphorus (P) and nitrogen (N) uptake, and P absorption rate of maize seedlings when supplied at different concentrations (2.5, 5, 10, and 25 mg C L −1 ) in the hydroponic culture. The root vigor, root plasma membrane H + -ATPase activity, and root nitrate reductase activity were also determined as the representative indicators of the root capacity for nutrient absorption, and used to clarify the mechanism by which PHA affects the maize growth and nutrient absorption. The results showed that the dry biomass, phosphorus uptake, nitrogen uptake, and average phosphorus absorption rates were significantly higher by 14.7–27.9%, 9.6–35.1%, 17.9–22.4%, and 22.1–31.0%, respectively, in plants treated with 2.5–5 mg C L −1 PHA compared to untreated controls. Application of 10–25 mg C L −1 raw HA resulted in similar stimulatory effects on plant growth and nutrient absorption. However, higher levels of PHA (10–25 mg C L −1 ) negatively impacted these indicators of plant growth. Furthermore, low PHA or high raw HA concentrations similarly improved root vigor and root plasma membrane H + -ATPase and nitrate reductase (NR) activities. These results indicate that lower concentrations of PHA can stimulate maize seedling growth and nutrient absorption to an extent that is comparable to the effect of higher concentrations of raw HA. Thus, the proportion of HA incorporated into HAP could be lower than the theoretical amount estimated through assays evaluating the biological effects of raw HA.
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Claims 9
Claim · model-written summary
Low PHA increased maize dry biomass by 14.7–27.9%.
- Treatment
- 2.5–5 mg C L −1 PHAe0
- Outcome
- dry biomasse0
- Direction
- highere0
- Value
- 14.7–27.9%e0
- Comparator
- untreated controlse0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dry biomass, phosphorus uptake, nitrogen uptake, and average phosphorus absorption rates were significantly higher by 14.7–27.9%, 9.6–35.1%, 17.9–22.4%, and 22.1–31.0%, respectively, in plants treated with 2.5–5 mg C L −1 PHA compared to untreated controls.”
t0“Maize”
Claim · model-written summary
Low PHA increased phosphorus uptake by 9.6–35.1%.
- Treatment
- 2.5–5 mg C L −1 PHAe0
- Outcome
- phosphorus uptakee0
- Direction
- highere0
- Value
- 9.6–35.1%e0
- Comparator
- untreated controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dry biomass, phosphorus uptake, nitrogen uptake, and average phosphorus absorption rates were significantly higher by 14.7–27.9%, 9.6–35.1%, 17.9–22.4%, and 22.1–31.0%, respectively, in plants treated with 2.5–5 mg C L −1 PHA compared to untreated controls.”
t0“Maize”
Claim · model-written summary
Low PHA increased nitrogen uptake by 17.9–22.4%.
- Treatment
- 2.5–5 mg C L −1 PHAe0
- Outcome
- nitrogen uptakee0
- Direction
- highere0
- Value
- 17.9–22.4%e0
- Comparator
- untreated controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dry biomass, phosphorus uptake, nitrogen uptake, and average phosphorus absorption rates were significantly higher by 14.7–27.9%, 9.6–35.1%, 17.9–22.4%, and 22.1–31.0%, respectively, in plants treated with 2.5–5 mg C L −1 PHA compared to untreated controls.”
t0“Maize”
Claim · model-written summary
Low PHA increased average phosphorus absorption by 22.1–31.0%.
- Treatment
- 2.5–5 mg C L −1 PHAe0
- Outcome
- average phosphorus absorption ratese0
- Direction
- highere0
- Value
- 22.1–31.0%e0
- Comparator
- untreated controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“dry biomass, phosphorus uptake, nitrogen uptake, and average phosphorus absorption rates were significantly higher by 14.7–27.9%, 9.6–35.1%, 17.9–22.4%, and 22.1–31.0%, respectively, in plants treated with 2.5–5 mg C L −1 PHA compared to untreated controls.”
t0“Maize”
Claim · model-written summary
High raw HA stimulated maize growth and nutrient absorption similarly.
- Treatment
- 10–25 mg C L −1 raw HAe0
- Outcome
- plant growth and nutrient absorptione0
- Direction
- stimulatorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of 10–25 mg C L −1 raw HA resulted in similar stimulatory effects on plant growth and nutrient absorption.”
t0“Maize”
Claim · model-written summary
High PHA negatively affected maize growth indicators.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher levels of PHA (10–25 mg C L −1 ) negatively impacted these indicators of plant growth.”
t0“Maize”
Claim · model-written summary
Low PHA improved root vigor and two root enzyme activities.
- Treatment
- low PHAe0
- Outcome
- root vigor and root plasma membrane H + -ATPase and nitrate reductase (NR) activitiese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low PHA or high raw HA concentrations similarly improved root vigor and root plasma membrane H + -ATPase and nitrate reductase (NR) activities.”
t0“Maize”
Claim · model-written summary
High raw HA improved root vigor and two root enzyme activities.
- Treatment
- high raw HA concentrationse0
- Outcome
- root vigor and root plasma membrane H + -ATPase and nitrate reductase (NR) activitiese0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low PHA or high raw HA concentrations similarly improved root vigor and root plasma membrane H + -ATPase and nitrate reductase (NR) activities.”
t0“Maize”
Claim · model-written summary
Authors suggest less HA may be incorporated into HAP than raw HA assays indicate.
- Outcome
- proportion of HA incorporated into HAP could be lower than the theoretical amounte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the proportion of HA incorporated into HAP could be lower than the theoretical amount estimated through assays evaluating the biological effects of raw HA.”
t0“Maize”
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