Paper · 2026 · Agriculture

Integrated Assessment of Vermiculite Enriched with Humic Substances or Chlorella vulgaris: Soil Fertility, Maize Nutrition, and Microbial Indicators

Galymzhan Saparov; Kanat Kulymbet; Bakhytbek Amirov; et al.

7
claims
14
verbatim quotes
0
citations
3
research areas
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Open access · cc-by

Abstract

Maize production in semi–arid irrigated systems depends on soil fertility and an active rhizosphere. We hypothesized that vermiculite enriched with humic substances (HS) or Chlorella vulgaris (CV) would outperform vermiculite alone by improving soil fertility, maize nutrition, and rhizosphere-associated microbial indicators. A field experiment was conducted in southern Kazakhstan under medium–loam sierozem using a randomized block design with three replicates and seven treatments: control, vermiculite at 1 and 2 t ha−1, vermiculite + HS at 1 and 2 t ha−1, and vermiculite + CV at 1 and 2 t ha−1. Amendments were incorporated before sowing, and soil, plant, and microbial measurements were taken before sowing, at V6–V8, and after harvest. Compared with the control, all amendments improved early maize growth, leaf area development, biomass accumulation, and nutrient status, and increased grain yield. The strongest response was obtained with vermiculite + HS at 2 t ha−1, which increased grain yield from 6.48 to 10.24 t ha−1 (+58%). Microbial indicators differed between bulk soil and the rhizosphere, while Pearson correlation and PCA revealed coordinated soil–plant–microbe responses and productivity–linked variables across treatments. Taken together, these results indicate that enriched vermiculite, especially HS–enriched vermiculite at 2 t ha−1, is a promising amendment for improving maize productivity and rhizosphere functioning in semi–arid irrigated systems.

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

All amendments improved early maize growth, leaf area, and biomass relative to the control.

Taxon
maizeZea mayst0
Treatment
all amendmentse0
Outcome
early maize growth, leaf area development, biomass accumulatione0
Direction
improvede0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Compared with the control, all amendments improved early maize growth, leaf area development, biomass accumulation”

✓ located verbatim in the source · abstract, characters 745–859 · show in text ↑
t0

“Integrated Assessment of Vermiculite Enriched with Humic Substances or Chlorella vulgaris: Soil Fertility, Maize Nutrition, and Microbial Indicators”

✓ located verbatim in the source · title, characters 0–148 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

All amendments improved maize nutrient status relative to the control.

Taxon
maizeZea mayst0
Treatment
all amendmentse0
Outcome
nutrient statuse0
Direction
improvede0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Compared with the control, all amendments improved early maize growth, leaf area development, biomass accumulation, and nutrient status”

✓ located verbatim in the source · abstract, characters 745–880 · show in text ↑
t0

“Integrated Assessment of Vermiculite Enriched with Humic Substances or Chlorella vulgaris: Soil Fertility, Maize Nutrition, and Microbial Indicators”

✓ located verbatim in the source · title, characters 0–148 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

All amendments increased maize grain yield relative to the control.

Taxon
maizeZea mayst0
Treatment
all amendmentse0
Outcome
grain yielde0
Direction
increasede0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Compared with the control, all amendments improved early maize growth, leaf area development, biomass accumulation, and nutrient status, and increased grain yield”

✓ located verbatim in the source · abstract, characters 745–907 · show in text ↑
t0

“Integrated Assessment of Vermiculite Enriched with Humic Substances or Chlorella vulgaris: Soil Fertility, Maize Nutrition, and Microbial Indicators”

✓ located verbatim in the source · title, characters 0–148 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Vermiculite plus humic substances at 2 t ha−1 increased maize grain yield from 6.48 to 10.24 t ha−1, or 58%.

Taxon
maizeZea mayst0
Treatment
vermiculite + HS at 2 t ha−1e0
Outcome
grain yielde0
Direction
increasede0
Value
10.24 t ha−1e0
Qualifier
+58%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“vermiculite + HS at 2 t ha−1, which increased grain yield from 6.48 to 10.24 t ha−1 (+58%)”

✓ located verbatim in the source · abstract, characters 950–1040 · show in text ↑
t0

“Integrated Assessment of Vermiculite Enriched with Humic Substances or Chlorella vulgaris: Soil Fertility, Maize Nutrition, and Microbial Indicators”

✓ located verbatim in the source · title, characters 0–148 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Microbial indicators differed between bulk soil and maize rhizosphere.

Taxon
maizeZea mayst0
Organ / stage
rhizospheree0
Outcome
Microbial indicatorse0
Direction
differede0
Comparator
bulk soile0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Microbial indicators differed between bulk soil and the rhizosphere”

✓ located verbatim in the source · abstract, characters 1042–1109 · show in text ↑
t0

“Integrated Assessment of Vermiculite Enriched with Humic Substances or Chlorella vulgaris: Soil Fertility, Maize Nutrition, and Microbial Indicators”

✓ located verbatim in the source · title, characters 0–148 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Pearson correlation and PCA found coordinated soil, maize, and microbe responses across treatments.

Taxon
maizeZea mayst0
Outcome
coordinated soil–plant–microbe responses and productivity–linked variablese0
Qualifier
across treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Pearson correlation and PCA revealed coordinated soil–plant–microbe responses and productivity–linked variables across treatments”

✓ located verbatim in the source · abstract, characters 1117–1246 · show in text ↑
t0

“Integrated Assessment of Vermiculite Enriched with Humic Substances or Chlorella vulgaris: Soil Fertility, Maize Nutrition, and Microbial Indicators”

✓ located verbatim in the source · title, characters 0–148 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors identify enriched vermiculite, especially humic-substance enriched vermiculite at 2 t ha−1, as promising for maize productivity and rhizosphere functioning.

Taxon
maizeZea mayst0
Treatment
HS–enriched vermiculite at 2 t ha−1e0
Outcome
improving maize productivity and rhizosphere functioninge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“enriched vermiculite, especially HS–enriched vermiculite at 2 t ha−1, is a promising amendment for improving maize productivity and rhizosphere functioning”

✓ located verbatim in the source · abstract, characters 1292–1447 · show in text ↑
t0

“Integrated Assessment of Vermiculite Enriched with Humic Substances or Chlorella vulgaris: Soil Fertility, Maize Nutrition, and Microbial Indicators”

✓ located verbatim in the source · title, characters 0–148 · show in text ↑

growing medium · yield · Zea mays (maize)Positive and negative results

36 papers: 32 positive, 4 negative or no effect

31 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
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other · nutrient: content · Zea mays (maize)Positive and negative results

119 papers: 107 positive, 12 negative or no effect

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other · growth · Zea mays (maize)Positive and negative results

184 papers: 164 positive, 20 negative or no effect

163 papers in the same direction as this paper · 20 in the opposite direction

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other · yield · Zea mays (maize)Positive and negative results

253 papers: 221 positive, 32 negative or no effect

220 papers in the same direction as this paper · 26 in the opposite direction · 6 reporting no difference from an alternative

  1. no effect
  2. no effect
  3. no effect

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.