Abstract
Cyclotides are macrocycle peptides produced by plants from several families, including Violaceae. These compounds have the potential for applications in medicine, bioengineering and crop protection thanks to their multiple biological activities. In most cases, cyclotides are extracted from plant material. Plant cell culture provides a viable and sustainable form of plant biomass production Cyclotides are host defense peptides. The aim of the current study was to test whether different plant stress hormones and biological elicitors have effects on cyclotide production in Viola uliginosa suspension cultures. Different concentrations of jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA) and neutralized pathogens were tested. The cyclotide production was assessed using MALDI-MS. Five major peptides produced by V. uliginosa cultures were chosen for analysis, of which one was sequenced de novo. The treatments had little influence on the suspension’s growth, with the exception of 100 μM SA, which enhanced the biomass increase, and 100 μM ABA, which was toxic. Significant increases in the production of three cyclotides (viul M, cyO13 and cyO3) were observed in suspensions primed with JA (50 μM, 100 μM, 200 μM) after 14 days of culturing. Biotic elicitors had no observable effect on cyclotide production. The current study indicates that some cyclotides in V. uliginosa are triggered in response to JA. The stress plant hormones can be used to enhance plant cell culture-based production systems.
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Claims 8
Claim · model-written summary
One of five major peptides from V. uliginosa cultures was sequenced de novo.
- Taxon
- V. uliginosa
Viola uliginosae0 - Outcome
- one was sequenced de novoe0
- Value
- onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Five major peptides produced by V. uliginosa cultures were chosen for analysis, of which one was sequenced de novo.”
Claim · model-written summary
Treatments generally had little influence on suspension growth.
- Taxon
- Viola uliginosaViola uliginosat0
- Treatment
- treatmentse0
- Outcome
- suspension’s growthe0
- Direction
- little influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The treatments had little influence on the suspension’s growth, with the exception of 100 μM SA, which enhanced the biomass increase, and 100 μM ABA, which was toxic.”
t0“Viola uliginosa”
Claim · model-written summary
100 μM SA enhanced culture biomass increase.
- Taxon
- Viola uliginosaViola uliginosat0
- Treatment
- 100 μM SAe0
- Outcome
- biomass increasee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The treatments had little influence on the suspension’s growth, with the exception of 100 μM SA, which enhanced the biomass increase, and 100 μM ABA, which was toxic.”
t0“Viola uliginosa”
Claim · model-written summary
100 μM ABA was toxic to the cultures.
- Taxon
- Viola uliginosaViola uliginosat0
- Treatment
- 100 μM ABAe0
- Outcome
- toxice0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The treatments had little influence on the suspension’s growth, with the exception of 100 μM SA, which enhanced the biomass increase, and 100 μM ABA, which was toxic.”
t0“Viola uliginosa”
Claim · model-written summary
JA increased viul M production after 14 days.
- Taxon
- Viola uliginosaViola uliginosat0
- Treatment
- JA (50 μM, 100 μM, 200 μM)e0
- Outcome
- production of three cyclotides (viul M, cyO13 and cyO3)e0
- Direction
- increasese0
- Qualifier
- after 14 days of culturinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significant increases in the production of three cyclotides (viul M, cyO13 and cyO3) were observed in suspensions primed with JA (50 μM, 100 μM, 200 μM) after 14 days of culturing.”
t0“Viola uliginosa”
Claim · model-written summary
Biotic elicitors had no observable effect on cyclotide production.
- Taxon
- Viola uliginosaViola uliginosat0
- Treatment
- Biotic elicitorse0
- Outcome
- cyclotide productione0
- Direction
- no observable effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Biotic elicitors had no observable effect on cyclotide production.”
t0“Viola uliginosa”
Claim · model-written summary
Some V. uliginosa cyclotides are triggered in response to JA.
- Taxon
- V. uliginosaViola uliginosae0
- Treatment
- JAe0
- Outcome
- some cyclotidese0
- Qualifier
- triggered in response to JAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The current study indicates that some cyclotides in V. uliginosa are triggered in response to JA.”
Claim · model-written summary
Stress plant hormones can enhance plant cell culture production systems.
- Taxon
- Viola uliginosaViola uliginosat0
- Outcome
- enhance plant cell culture-based production systemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The stress plant hormones can be used to enhance plant cell culture-based production systems.”
t0“Viola uliginosa”