Abstract
Due to the impaired antiviral RNAi, the dcl2dcl4 (dcl2/4) mutant is highly susceptible to viruses deficient of the viral suppressor of the RNA silencing (VSR) contrast to wild-type Arabidopsis. It was found that more severe disease symptoms were induced in dcl2/4 infected with VSR-deficient CMV (CMV-Δ2b or CMV-2aTΔ2b) compared to wild-type Arabidopsis infected with intact CMV. In order to investigate the underlying mechanism, comparative transcriptome analysis was performed with Col-0 and dcl2/4 that were infected by CMV, CMV-Δ2b and CMV-2aTΔ2b, respectively. Our analysis showed that the systematic infection of CMV, CMV-Δ2b and CMV-2aTΔ2b could cause hypoxia response and reduce photosynthesis. Asymptomatic infections of CMV-Δ2b or CMV-2aTΔ2b in Columbia (Col-0) promoted the expression of cell division-related genes and suppressed the transcription of metabolism and acquired resistance genes. On the other hand, immunity and resistance genes were highly induced, but photosynthesis and polysaccharide metabolism-related genes were suppressed in diseased plants. More interestingly, cell wall reorganization was specifically caused in modestly diseased Col-0 infected by CMV and a strong activation of SA signaling were correspondingly induced in severely diseased dcl2/4 by CMV or CMV mutants. Thus, our research revealed the nature of the Arabidopsis–CMV interaction at the transcriptome level and could provide new clues in symptom development and antiviral defense in plants.
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Claims 9
Claim · model-written summary
VSR-deficient CMV induced more severe disease symptoms in dcl2/4 than intact CMV in wild-type Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Material
- dcl2/4e0
- Treatment
- VSR-deficient CMVe0
- Interactor
- CMV
cucumber mosaic cucumoviruse0pathogen - Outcome
- disease symptomse0
- Direction
- more severee0
- Comparator
- wild-type Arabidopsis infected with intact CMVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“It was found that more severe disease symptoms were induced in dcl2/4 infected with VSR-deficient CMV (CMV-Δ2b or CMV-2aTΔ2b) compared to wild-type Arabidopsis infected with intact CMV”
Claim · model-written summary
Asymptomatic CMV-Δ2b or CMV-2aTΔ2b infection in Col-0 promoted expression of cell division-related genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Columbia (Col-0)e1
- Treatment
- Asymptomatic infections of CMV-Δ2b or CMV-2aTΔ2be1
- Interactor
- CMV-Δ2be1pathogen
- Outcome
- expression of cell division-related genese1
- Direction
- promotede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“Asymptomatic infections of CMV-Δ2b or CMV-2aTΔ2b in Columbia (Col-0) promoted the expression of cell division-related genes and suppressed the transcription of metabolism and acquired resistance genes”
Claim · model-written summary
Asymptomatic CMV-Δ2b or CMV-2aTΔ2b infection in Col-0 suppressed transcription of metabolism and acquired resistance genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Columbia (Col-0)e1
- Treatment
- Asymptomatic infections of CMV-Δ2b or CMV-2aTΔ2be1
- Interactor
- CMV-Δ2be1pathogen
- Outcome
- transcription of metabolism and acquired resistance genese1
- Direction
- suppressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“Asymptomatic infections of CMV-Δ2b or CMV-2aTΔ2b in Columbia (Col-0) promoted the expression of cell division-related genes and suppressed the transcription of metabolism and acquired resistance genes”
Claim · model-written summary
In diseased plants, immunity and resistance genes were highly induced.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- diseased plantse2
- Outcome
- immunity and resistance genese2
- Direction
- highly inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“On the other hand, immunity and resistance genes were highly induced, but photosynthesis and polysaccharide metabolism-related genes were suppressed in diseased plants”
Claim · model-written summary
In diseased plants, photosynthesis and polysaccharide metabolism-related genes were suppressed.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- diseased plantse2
- Outcome
- photosynthesis and polysaccharide metabolism-related genese2
- Direction
- suppressede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“On the other hand, immunity and resistance genes were highly induced, but photosynthesis and polysaccharide metabolism-related genes were suppressed in diseased plants”
Claim · model-written summary
Systematic CMV infection could cause a hypoxia response in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- systematic infection of CMV, CMV-Δ2b and CMV-2aTΔ2be3
- Interactor
- CMVcucumber mosaic cucumoviruse3pathogen
- Outcome
- hypoxia responsee3
- Direction
- causee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e3“Our analysis showed that the systematic infection of CMV, CMV-Δ2b and CMV-2aTΔ2b could cause hypoxia response and reduce photosynthesis”
Claim · model-written summary
Systematic CMV infection reduced photosynthesis in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- systematic infection of CMV, CMV-Δ2b and CMV-2aTΔ2be3
- Interactor
- CMVcucumber mosaic cucumoviruse3pathogen
- Outcome
- photosynthesise3
- Direction
- reducee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e3“Our analysis showed that the systematic infection of CMV, CMV-Δ2b and CMV-2aTΔ2b could cause hypoxia response and reduce photosynthesis”
Claim · model-written summary
Cell wall reorganization was specifically caused in modestly diseased Col-0 infected by CMV.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Col-0e4
- Treatment
- infected by CMVe4
- Outcome
- cell wall reorganizatione4
- Direction
- causede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e4“More interestingly, cell wall reorganization was specifically caused in modestly diseased Col-0 infected by CMV and a strong activation of SA signaling were correspondingly induced in severely diseased dcl2/4 by CMV or CMV mutants”
Claim · model-written summary
SA signaling was strongly activated in severely diseased dcl2/4 infected by CMV or CMV mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- dcl2/4e4
- Treatment
- CMV or CMV mutantse4
- Outcome
- SA signalinge4
- Direction
- inducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e4“More interestingly, cell wall reorganization was specifically caused in modestly diseased Col-0 infected by CMV and a strong activation of SA signaling were correspondingly induced in severely diseased dcl2/4 by CMV or CMV mutants”
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